Introduction: Intraperitoneal tumor seeding is a common form of recurrence after surgery for GI tumours. Early diagnosis and treatment may influence the course of disease. Hypothesis and objectives of the work The finding of free tumor cells and / or elevation of tumor markers in peritoneal fluid predicts intraperitoneal tumor recurrence. The results of these tests can be used to indicate HIPEC. Aim of this work is to determine the sensitivity and specificity of cytology and tumor markers levels and importance of these tests for early diagnosis and prognosis of recurrence. Another objective was to confirm the applicability of RT PCR for identification of free tumor cells. Patients and methods . Materials are sourced both during initial surgery (220 patients) and by the dispensary controls (25 patients). The peritoneal fluid was examined cytologically and biochemically too. 50 samples was exemined by RT PCR. Results Elevation of tumor markers or finding of free intraperitoneal tumor cells predicts recurrence. The prognosis of these patients corresponds to stage IV TNM classification, regardless of other parameters TNM. Low sensitivity of these tests (34% for cytology, 53% for tumor markers) does not allow its use as an indicator of HIPEC. Due to degradation of RNA we did not confirm the value of RT...

The diploma thesis deals with the protection of personnel in cytoreductive surgery and hyperthermic intraperitoneal chemotherapy, focusing on non-medical healthcare workers - anesthetist nurses. The aim of the work was to analyze the knowledge of anesthesia nurses in the use of personal protective equipment, the specifics of selection for the given performance, the use of an electrocautery with integrated suction, the availability of decontamination aids In case of an accident of cytotoxic substances and if the staff is afraid of their own health. The data was obtained through a questionnaire. For the research were selected anesthesia nurses working in operating theaters in selected health care facilities in the Czech Republic. The total number of respondents who participated in the research was 35. The results of the survey revealed that the staff did not find out what PPEs are recommended for this type of operation. It's either because the staff is less linguistically equipped or does not know EBN / EBP or EBM. The staff only uses those PPEs that are available to them. As a good result, 49% of respondents use an integrated electric exhaust system. With regard to the availability of decontamination aids, I have found that most of the staff does not know whether these utilities are available at...