The subject of my diploma thesis is the processing of project documentation of the construction part for the realization of the new build private health centre Proficlinic in the outskirts of České Budějovice. It is a two-storey partially basement building. On the first floor there is a pharmacy with the preparation of cytostatics, surgery x-ray and facilities for staff. On the underground floor there are technical facilities for new buildings and storage facilities. On the second floor there are individual surgeries and day care center of oncology. The ceilings are made of prestressed panels. The roof of the building is flat single-skinned.

This diploma thesis is focused on preparation of liposome by relatively new method called nanofluidisation. This method allows the controlled preparation of small unilamellar liposomes in one step. In my thesis I was dealing with optimalization of liposomes preparation which carry hydrophobic cytostatics using this method. Cytotoxic effect of liposomes carrying hydrophobic cytostatics in vitro on cell lines A549 and MCF-7 was determined. In cytotoxicyty test I compared the effect of hydrophobic cytostatics (paclitaxel and derivates of vitamin E specifically alfa-Tos, alfa-TEA) that were incorporated into liposomes prepared via nanofluidisation method and lipid film hydration method. Moreover, a technology of lyophilisation in the presence of cryoprotectants for preparation of liposomes using the method of nanofluidisation was developed.