During 1994-1996, reproductive biology and denning ecology of black bears (<i>Ursus americanus</i>) were investigated in the George Washington and Jefferson National Forests, Virginia. The average age of captured bears was 4.88 years (SE=0.43, n=64) and 2.72 years (SE=0.19, n=127) for females and males, respectively. Timing of estrus ranged from 21 June to 7 August with most (58.8%, n=10) estrus females captured between 15 July and 31 July. Ages of primiparity were 3 or 4 years and averaged 3.14 years (SE=0.14, n=7). Average litter size was 2.0 cubs per litter (n=26). During 1995 and 1996, respectively, 81.8% and 83.3% of potentially pregnant females reproduced. Date of parturition averaged 22 January in 1995 and 1996, but ranged from 1 January to 22 February in 1995 and 11 January to 14 February in 1996. Pregnant females did not enter dens earlier (T=0.74, P=0.4598) than other bears, and did not emerge later (T=-1.69, P=0.0925) than other bears. cavities in trees and snags composed 82.4% (n=56) of all dens. Red oak (<i>Quercus rubra</i>) and chestnut oak (<i>Quercus prinus</i>) were the most commonly (n=49) used den tree species. Tree dens were on sites with steeper slopes (t=3.41, P=0.0013), lower medium tree density (t=-2.22, P=0.0313), higher large tree density (t=2.24, P=0.0296), and lower stem density (t=-3.43, P=0.0013) than random sites. Ground dens were on steeper slopes t=3. 22, P=O. 0122) than random sites. Den reuse occurred at 2 of 17 (11.8%) dens, and 84.6% (11 of 13) of bears, monitored both winters, used the same den type during both winters. Virginia's black bear harvest during 1982-1994 was used to reconstruct Virginia's bear population. During 1982-1991, Virginia I s reconstructed male black bear population ranged from 880 bears to 1,241 bears. Numbers of males increased during 1982-1986, declined during 1987-1989, and increased during 1990-1991. Virginia's reconstructed female black bear population ranged from 943 bears to 1064 bears and increased most years. / Master of Science