Thesis (M.S.)--Oregon State University, 2004. / Includes bibliographical references (leaves 37-42). Also available online (PDF file) by a subscription to the set or by purchasing the individual file.

Thesis (Ph. D.)--University of Massachusetts Amherst, 2009. / Includes bibliographical references (p. 169-191). Print copy also available.

Thesis (M. S.)--University of North Carolina at Greensboro, 2006. / Title from PDF title page screen. Advisor: Cheryl Lovelady; submitted to the School of Human Environmental Sciences. Includes bibliographical references (p. 51-55).

Thesis (M.S.)--Oregon State University, 2004. / Includes bibliographical references (leaves 37-42).

Thesis (M.S.)--Ball State University, 2005. / Includes bibliographical references (leaves 45-49). Also available online (PDF file) by a subscription to the set or by purchasing the individual file.

Thesis (M.S.)--Ball State University, 2005. / Includes bibliographical references (leaves 45-49).

Made available in DSpace on 2014-06-11T19:26:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-11-07Bitstream added on 2014-06-13T18:30:29Z : No. of bitstreams: 1 figueira_pt_me_botfmvz.pdf: 461601 bytes, checksum: 904bcd42f1e51dec1dd8f5d74349b902 (MD5) / Universidade Estadual Paulista (UNESP) / Na busca pela excelência sensorial de um produto, as características exigidas pelo consumidor são a maciez, a suculência e o odor, que estão diretamente ligadas a constituição proteica do produto, juntamente com os lipídeos e os carboidratos. Atualmente, o consumo de produtos cárneos no Brasil encontrase em constante crescimento, sendo que os de origem avícola são os principais expoentes. Para a análise da qualidade dos produtos, a avaliação da origem das proteínas musculares é fator de grande importância e algumas das técnicas que podem ser citadas para sua avaliação e quantificação são as técnicas eletroforéticas e densitométricas, muito utilizadas hoje como padrão para diversas pesquisas em alimentos. Para realização deste trabalho, partiuse de que as proteínas musculares de frangos, perus e avestruzes apresentam diferentes características estruturais devido à expressão gênica e suas estruturas primárias podem ser identificadas por técnicas eletroforéticas e, através da densitometria, quantificadas. Foram colhidos fragmentos de musculo peitoral de 10 aves de cada espécie abatidas em frigoríficos inspecionados. Realizou-se a extração proteica por maceração e centrifugação dos fragmentos, e posterior submissão a quatro técnicas eletroforéticas sendo elas a Eletroforese Nativa em Gel de Poliacrilamida, SDS-PAGE Não Denaturante, CELM-GEL® - Filme de Agarose Geral e Isoeletrofocalização em Phast-Gel. Os resultados obtidos demonstraram a obtenção de um padrão proteico para as espécies em estudo, podendo utilizá-las para diferenciação das espécies e com a densitometria demonstrou a ocorrência de variações quantitativas individuais que não comprometeram a padronização específica dos eletroferogramas / In pursuit of a sensory product excellence, the characteristics required by consumers are tenderness, juiciness and smells, and all then linked directly to protein constitution of the product, along with lipids and carbohydrates. Currently, the consumption of meat products in Brazil is constantly growing, and the poultry origins are the main exponents. For the quality analysis of product, the evaluation of the origin of the proteins muscles is a factor of great importance and some of the techniques can be cited for their evaluation and quantification are the electrophoretic and densitometric techniques, widely used today as a standard for research on all food. For this study, we started with the muscle proteins of chickens, turkeys and ostriches have different characteristics due to structural gene expression and their primary structures be identified by electrophoretic techniques and by densitometry, it is quantified. Fragments from pectoral muscle were collected front of 10 birds of each species slaughtered in abattoirs inspected. The protein extraction was carried out by the maceration and centrifugation the fragments, and subsequent submission to four electrophoretic techniques with them: the Native Gel Electrophoresis, Polyacrylamide, SDS-PAGE not Denaturant, CELM-GEL ® - Film Agarose General and IEF in Phast-gel. The results obtained demonstrate the attainment of a standard protein for the species under review and may use them for differentiation of species and densitometry demonstrated the occurrence of individual quantitative variations did not affect the specific standardization of electropherograms

X-ray densitometry was used to investigate some environmental and genetic influences on wood formation in young Douglas-fir trees growing in the Pacific Northwest. Sampling methods were determined from sectional analyses of even-aged stems and eleven growth-ring width and density variables were measured in several trials to elucidate' some of the causes of variation. Breast height increment core samples were shown to giveve a good representation of stand properties and juvenile-mature correlations for a 40-year-old crop confirmed the validity of determining intra-ring density parameters on young material. Increment core samples from the Co-operative Douglas-fir Provenance Test (5 locations; 5 provenances/location) were used for both gravimetric and densi-tometric analyses. The major components of variation were found to be stand location and the individual tree effect. Provenances consistently accounted for less than 5% of the total variation. Genotype-environment interaction was shown to be small for all properties measured with the exception of the intra-ring density range. Between sites, earlywood widths were more variable than latewood widths, but latewood density properties (mean latewood and ring maximum densities) were more variable than those for earlywood (mean earlywood and ring minimum densities). Earlywood and latewood parameters varied independently of one another. It was suggested that genetic control was weak at the provenance level but strong for individual trees. Regression analyses using growth-ring components and monthly weather data for the outer five rings at each site uncovered some highly significant effects which helped to explain the observed year-to-year variation. Density variables were less affected by weather conditions than earlywood and latewood widths. An examination of eight ramets from each of ten 13-year-old clones revealed highly significant differences in all eleven intra-ring parameters. Heritability estimates for individual growth rings showed a regular increase with tree age, and latewood properties (width, density and ring maximum density) were found to be under strong genetic control. On a clone-mean basis, density was not related significantly to growth rate, so that vigour and density properties could be selected for independently. Crown phenology (flushing and shoot growth characteristics) was not strongly correlated with growth-ring parameters, although there was an indication that early flushing may be associated with higher latewood density. A study of four parent trees and their control-pollinated progeny proved unsatisfactory due to lack of adequate replication and atypical stand conditions, but nevertheless provided a vehicle for discussion of problems involved in assessing plus-tree wood quality and narrow-sense heritabilities. The combined results were discussed in terms of the genecology of Douglas fir and the implications for forest management and utilization. / Forestry, Faculty of / Graduate

Using micro-computed tomography it is possible to detect the presence of pathologies which alter the lung's normal density. The density of the lungs can be altered depending on the amount of air, tissue, cells or fluid they contain. Using established mouse models of house dust mite (HDM) induced asthma, TGF-J31 induced pulmonary fibrosis (PF) and lipopolysaccharide (LPS) induced neutrophilic inflammation, this thesis examines if CT densitometry can distinguish between different pathophysiological processes. An airway segmentation method was applied to the CT images and data from these regions were assessed to determine: first, if pathologies can be detected compared to control animals; secondly, if pathological progression within each model can be measured; and finally, if it is possible to distinguish between the pathologies themselves. Lung histology and bronchoalveolar lavage fluid cytology, and total lung resistance (for the asthma model only) were assessed to confirm the disease models. The results showed that a healthy lung can be distinguished from a diseased lung in all three models. Pathological progression and resolution were also visible in the asthma and LPS groups. No changes were noted between the examined time points in the PF model. This corresponded to histological findings. It is also possible to distinguish between many of the pathologies based on the density profiles alone. Thus, CT densitometry affords a non-invasive method to longitudinally assess disease progression and resolution which is useful for the testing of novel therapeutics within the same subject. Regional CT density assessment, allows for the detection of localized pathologies around the airways which whole lung assessments may not be sensitive enough to detect. / Thesis / Master of Science (MSc)

published_or_final_version / abstract / Medicine / Doctoral / Doctor of Philosophy