This thesis is concerned with the development of MOS charge-coupled device (CCD) technology for future applications in X-ray astronomy. Of particular interest is increased detection efficiency of high energy X-ray photons and increased pixel readout speed for large area sensors. Chapter 2 reviews the generation of X-rays, methods for extra-terrestrial X-ray observations, detectors and provides an overview of X-ray astronomy missions. Chapter 3 discusses the CCD and introduces some of the recent technological developments that improve their overall performance for optical and X-ray photon detection. Chapter 4 presents the basic laboratory equipment and methods used to carry out the experimental work of this thesis. Chapter 5 presents the characterisation of new high resistivity devices that were manufactured by e2v technologies during the work of this thesis. Chapter 6 describes a method for estimating the depletion depth of a CCD by analysing the X-ray event patterns that are generated in CCD image data. Chapter 7 presents the equipment developed and experimental measurements taken to evaluate the high energy X-ray quantum efficiency of a high resistivity CCD. Finally, Chapter 8 describes the ongoing development and characterisation of low noise ASICs that are intended for use in future X-ray astronomy missions.