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In this thesis, the knowledge about problematics of drinking water, with a focus on microbiology of drinking water, was summarized. The aim of this thesis was to analyse indicator microorganisms in ten samples of water. Four samples came from water wells, three samples were from water mains and there were three samples from bottled water. The microbiological analyze was conducted in two periods, namely in summer of 2018 and in winter of 2019. The results were evaluated in statistics and there was detected, if there is any difference in number of microorganisms in the summer and in the winter and also if there is a statistically significant difference between the given types of water. Most microorganisms were in raw water. Only one water of the wells water fulfilled microbiological requirements for drinking water. It was detected that there is no statistically significant difference in number of microorganisms in summer and in winter. In the total amount of microorganisms, there is a difference between water of wells and bottled water, and between water coming from water main and bottled water.

No description available.

The study is focused on the evaluation of crayfish physiological reaction on hygienically treated water with chlorine dioxide (ClO2). A patented non-invasive monitoring system was used for observation of crayfish cardiac and locomotor activity. Monitoring was conducted from February to August 2017 under conditions of private commercial enterprise "Pivovar Protivín" in Czech Republic. Adult individuals of signal crayfish, Pacifastacus leniusculus, were kept separately in water-flow aquariums directly after the water treatment device producing ClO2 in concentration from 0.01 to 0.29 mg.l-1. Observed crayfish response to the disinfectant varied among individuals which could be explained by a different functional state and individual reaction on stimuli. Diurnal rhythm of some crayfish was disturbed even at a lower concentrations of chlorine dioxide (0.01-0.2 mg.l-1), while higher concentrations affected all animals. In addition to that, higher levels of chlorine dioxide ( 0.2 mg.l-1) significantly increased mortality. Maximum concentrations (0.2-0.29 mg.l-1) were observed 28 times in total during 202 days of monitoring, which resulted in 25 mortality cases occurred several days after exposure. In average, mortality of crayfish occurred three-four weeks after stocking to the experimental system. Possible lethal concentration of ClO2, which caused animal mortality, is 0.2 mg.l-1. Results suggested that crayfish exposure to ClO2, obviously, negatively affect their physiological processes; however, further studies are needed to examine specific effects of chlorine dioxide on internal organs of crayfish.

The diploma thesis compares the effectiveness of disinfectants on the content of the lactic acid. The disinfectants used for the experimental part are formalin and BetaStab®. The content of glucose and lactic acid was observed and compared after processing conventional beet and bio-beet. Based on the analysis of the measured data, it was found that the highest disinfecting effect on the lactic acid content had formalin, the avearge lactic acid value was 275 mg/l. In experiment with BetaStab® disinfection the average value of the lactic acid was 350 mg/l. When the beet was processed without the use of disinfection, this average lactic acid was 371 mg/l and in the untreated treatment of the bio-beet, the lactic acid content was 467 mg/l. The glucose content of formalin disinfection diffusion juice reached an average of 573 mg/l. When BetaStab® disinfection was applied, the average glucose content was 348 mg/l and a similar value was measured for the bio-beet treatment (328 mg/l). The highest glucose content had untreated conventional beet, where an average value of 690 mg/l was reached.

Nosocomial infections, which do not often relate to the diseases are increasing nowadays. Clostridium difficile belongs to the frequent nosocomial infections and it is known as post-antibiotic colitis. The main reason of colitis is the usage of antibiotics, especially broad-spectrum antibiotics. The thesis is divided into the theoretical part and practical, as well. Theoretical part describes the division of the nosocomial infections, infection of the intestinal tract, anatomy, physiology of the intestines and infectious diarrhoeal diseases. The thesis is subsequently aimed to the clostridial infections and precautions against the spread of the disease. Practical part is aimed to the knowledge of the nurses, skills and attitude towards this issue.The thesis uses quantitative investigation and technique of the questionnaires, hidden observation of the nurses working on the selected wards and additional interviews with head nurses. The research was conducted in hospital in Tábor, a.s. The questionnaires were distributed on the surgery, orthopaedics, surgical JIP, ARO, ONP, infective ward, rehabilitative ward, TRN, cardio JIP, internal ward-cardio, internal ward-gastro. The thesis was formed from 143 questionnaires and 171 questionnaires were distributed. Hidden observation was made by head nurses from individual wards and it was logged to the relevant observation sheets.From existing findings we can say that there exist specifics of nursing care at the patient with the clostridium difficile. Among to these specifics we can cite the barrier nursing care where we can include the isolation of the patient, disinfection and hygiene of hands,using protectors, appropriate usage of laundries and infectious waste, location of the patient according to the epidemiological perpective and individualization of the tools for the patients. From another investigation ensue that the nurses keep barrier nursing care, superficial disinfecion, decontamination of the tools. From the results is evident that the nurses do not know the methods of the transmission of the clostridial infection. On the base of another investigations we have found out that the nurses do not know principles of the barrier nursing care. In conclusion is it possible to say that the nurses do not have so extensive information, that are essential for care for the patiens with clotridium difficile. In order to care for these patients in right way is neccessary to know principles of the barrier nursing care and keep them all. Keeping the principles of the barrier nursing care is crucial step in preventing the transmission nosocomial infections. The results will be provided to the officials of the individual hospital´s wards as an option of improvement in caring for the patiens with clostridial infection. The results were partially presented at a conference in Tabor´s hospital in May 2014. We recommend to re-train the staff of the hospital, which would be specifically aimed towards the principles of the barrier nursing care and towards the disinfection and decontamination in related to the nosocomial infections. On the base of these findings was made a proposal of the nursing care standard, which would specify and unite the care for the patiens with clostride infection. Subsequently, it would be apropriate to repeat the research in 1 2 years and than both researches compare together.

The degree work is concentrated on basic characterization of nosocomial infections, on the prevention thereof and on the observance of antiepidemical measures in the sphere of stomatology. Although these infections were largely related to the mortality and morbidity in health service establishments in the past, their occurrence goes down significantly these days. The adoption and observance of fundamental hygienic principles and rules was of decisive importance in this sphare. The neglect of these principles can endanger the health and life of the patient seriously end bring about the increase of costs for further therapy of the patient. It is therefore necessary even at present time ot pay great attention to these diseases and to the prevention thereof. The research part of the work is devoted to the evaluation of preventive measures connected with nosocomial infections in stomatological practice. The literature search in the theoretical part follows therefore this orientation too. The theoretical part deals at first with the most frequent agents of nosocomial infections, that can be encountered. The attention is concentrated on brief characterization of these diseases, their sources and on the process of spreading. Then, the basic classification of these diseases and the concrete methods of prevention are mentioned. In the sphere of stomatology, the work resumes the basic historical relations and identifies particular sectors of this discipline. In conneciton with nosocomial infections, their impact upon the stomatology as well as the need of protection against these infections are discussed. It means epidemiological measures, importance of hand hygiene, necessity of sterilization, desinfection and of cleaning. The aim of the work is to point out the importance of prevention as to the origin and spreading of these infections and the necessity to observe the epidemical principles at the work in dental surgeries. The performed investigation resumes established facts about the observance of hygienic and epidemiological regimen and that not only as to the general judgement on problems, but in consideration of local differences, of differences in the duration of practice carried out by individual stomatologists and in relation to the frequency of conducted hygienic inspections as well. Problematic areas as to the nosocomial infections and as to the observance of decreed provisions by stomatologists are defined here. It refers also to the influence of the locality and to the frequency of inspections directed to the observance of these hygienic rules by physicians.

The master thesis is focused on problems surrounding defects and deficiencies of running a swimming pool site. It deals with particular defects which can occur at swimming pool's site. Different public swimming pools in Brno were visited to conduct an evaluation of real defects and deficiencies. After conducting the overview of defects, a correction solution was design for each defect. The defects are ranked according a scale measuring danger which I created

The diploma thesis deals with problems of healthcare-associated infection in selected nursing activities. The thesis is a theoretical one; it has been processed by means of review and synthesis. In this thesis, four objectives were set, namely to create a comprehensive overview of the problems of healthcare-associated infection in selected nursing activities. Other objectives were to map and analyze clinically relevant sites of transmission and possibilities of prevention of healthcare-associated infection in selected nursing activities and to propose recommendations for the prevention of these infections. The thesis is divided into several chapters that deal with the characteristics of healthcare-associated infection, focusing on the causative agents, formation, spreading and prevention. It also deals with particular nursing activities. The first chosen activity was the care of medical devices intended for repeated use, with a particular focus on the implementation of their disinfection. Secondly, hand sanitation as a basic factor affecting the transmission of healthcare-associated infection activity was selected. This chapter deals primarily with the hygienic hand disinfection, its indications, methods and implementation of disinfection products. It also deals with strategies for improving hand hygiene and glove use in providing nursing care. As the third nursing activity, aseptic techniques, which are an essential part in providing nursing care, were chosen. The thesis describes three specific clinical nursing procedures: aseptic collection of venous blood from a peripheral vein, preparation and administration of a drug into an existing peripheral venous catheter, and uncomplicated (aseptic) wounds dressing. Processing of the thesis was based on a range of relevant sources from the Czech Republic but also from abroad. It also contains various recommendations of national and international institutions. The thesis can serve as a basis for more effective prevention of healthcare-associated infection in certain areas.

The goal of the graduation theses was a comparison of the hygenic quality of raw cows milk, it was expressed by the value of total bacterial count (TBC) and somatic cells count (SCC) in four breeds of milking cows using only postdipping (3 breeds) in comparison with breed using predipping and postdipping. The breeds differed in technology of lairage and milking procedures, grazing and the size the herd. During two seasons 2006 till 2007 was discovered that the breeds using predipping and postdipping had significantly less average values of TBC (9,46.103.ml-1) in comparison with the breeds using only postdipping (15,97.103.ml-1), the difference was statistically significant (P = 0,007) on the level of significance P < 0,05. Also it was shown that SCC were below averages levels with using predipping and postdipping (293,47.103.ml-1) in comparison to breeds using only postdipping (312,06.103.ml-1), the difference was also statistically significant (P = 0,01) on the level of significance P < 0,05.