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The entire infection process was monitored over four seasons (2012-2015) by a targeted and systematic study of ring-necked pheasants and red-legged partridges from an intensive artificial breeding programme in the Czech Republic, with focus on studying the transmission dynamics throughout the breeding process, from hatching up to release of birds to open area, in connection with timing of appropriate and effective application of drugs. 1752 pooled excrement samples were examined (745 from pheasants and 1007 from partridges). Post-mortem examinations were performed on 625 dead birds (514 pheasants and 111 partridges). Main infections of A. rufa and P. colchicus comprised representatives of the protozoa genera Eimeria and Cryptosporidium; as well as nematodes Capillaria s.l., Heterakis gallinarum and Syngamus trachea. Identification of oocyst isolates of Cryptosporidium baileyi, C. meleagridis anf C. galli were supported and characterized by nested PCR of heat shock protein 70 (HSP70), actin and 60 kDa glycoprotein (GP60) genes.

Background: More than 90 % of anal cancers are caused by high-risk human papillomavirus (HR HPV) infection and a history of cervical intraepithelial neoplasia (CIN) and cervical cancer is established as possible risk factor. The aim of this study was to demonstrate relationship between anal and cervical HPV infection in women with different grades of CIN and microinvasive cervical cancer and to determinate potential risk factors for concurrent cervical-anal HPV infection. Methods: A total of 272 women were enrolled in the study. The study group included 172 women who underwent conization for high-grade CIN or microinvasive cervical cancer. The control group consisted of 100 women with non-neoplastic gynecologic diseases or biopsy- confirmed CIN 1. All participants completed a questionnaire detailing their medical history and sexual risk factors and were subjected to anal and cervical HPV genotyping using Lynear array test (Roche). Results: Cervical, anal, and concurrent cervical-anal HPV infections were detected in 82.6 %, 48.3 % and 42.4 % of women in the study group, and in 28.0 %, 26.0 % and 8.0 % of women in the control group, respectively. The prevalence of the HR HPV genotypes was higher in the study group and significantly increased with the severity of cervical lesion. Concurrent infections...

Background: More than 90 % of anal cancers are caused by high-risk human papillomavirus (HR HPV) infection and a history of cervical intraepithelial neoplasia (CIN) and cervical cancer is established as possible risk factor. The aim of this study was to demonstrate relationship between anal and cervical HPV infection in women with different grades of CIN and microinvasive cervical cancer and to determinate potential risk factors for concurrent cervical-anal HPV infection. Methods: A total of 272 women were enrolled in the study. The study group included 172 women who underwent conization for high-grade CIN or microinvasive cervical cancer. The control group consisted of 100 women with non-neoplastic gynecologic diseases or biopsy- confirmed CIN 1. All participants completed a questionnaire detailing their medical history and sexual risk factors and were subjected to anal and cervical HPV genotyping using Lynear array test (Roche). Results: Cervical, anal, and concurrent cervical-anal HPV infections were detected in 82.6 %, 48.3 % and 42.4 % of women in the study group, and in 28.0 %, 26.0 % and 8.0 % of women in the control group, respectively. The prevalence of the HR HPV genotypes was higher in the study group and significantly increased with the severity of cervical lesion. Concurrent infections...

Attitudes and beliefs on HPV infection and vaccination II Veronika Regnerová, PharmDr. Eva Zimčíková, Ph.D. Background: Cervical cancer is the second most common type of cancer in women. The majority of cervical cancer cases are caused by human papillomavirus (HPV). Three vaccines have been approved to prevent HPV infection and related diseases. Objectives: The study aimed to analyse the knowledge and attitudes regarding HPV, cervical cancer and HPV vaccination in secondary school students in the Czech Republic. Methods: The study was carried out in May 2017 at four secondary schools. A questionnaire used for data collection comprised 15 items including questions on demographic characteristics, knowledge about cervical cancer and HPV, vaccination coverage, HPV perceived susceptibility and seriousness, and beliefs on HVP vaccination. Results: A total of 667 students participated (mean age: 16.8 y ± 1.18; 63% female); 20.5% were smokers, 24.7% God believers. Most of the students (97.0%) heard about cervical cancer and penile cancer, significant proportion (68.3%) knew HPV was the causative factor. Half of the respondents (49.9%) have heard about vaccination against HPV. Among cervical cancer risk factors HPV infection was reported in 58.1% cases followed by promiscuity (14.5%), irregular...

Nozokomiální infekce jsou v současné době významnou příčinou vzrůstající morbidity a mortality ve všech zdravotnických zařízení. Neznalost či ignorance metod prevence nozokomiálních nákaz může vést k závažnému ohrožení zdraví a života pacienta a výraznému zvýšení nákladů na léčbu. Práce komplexně představuje problematiku nozokomiálních nákaz a jejich efektů na lůžkové zdravotnické zařízení. Pozornost je věnována charakteristice procesu šíření nákaz, popsání současného stavu ve sledování a prevenci nákaz a konečně popsání situace v jedné nemocnici okresního typu.

The thesis deals with localization and quantification of Sphaerospora molnari (Myxozoa) in common carp (Cyprinus carpio). Sphaerospora molnari is microscopic endoparasite which attacks various tissues and organs, preferentially parasitizing intracellularly (histozoic type) in a carp fry. In this work, we studied the course of parasite infection in the various tissues and organs of the host (blood, gills, skin, kidney, liver, and muscle) in laboratory conditions. The course of infection was detected using qPCR.

ATTITUDES AND KNOWLEDGE OF HPV INFECTION AND VACCINATION Author: Dominika Blašková Supervisor: PharmDr. Eva Zimčíková, Ph.D. INTRODUCTION Human papillomavirus (HPV) is a DNA virus. The virus causes benign and malignant diseases of men and women such as laryngeal papillomatosis, genital warts, oropharyngeal carcinoma, cervical cancer, penile cancer and anus cancer. HPV can be prevented by appropriate precautionary measures. The main preventative method is vaccination against HPV. Besides vaccination, regular screening is necessary to detect premalignant changes on time. AIMS The aim of the theoretical part of the diploma thesis was to summarize the information about HPV, the diseases caused by human papillomavirus and vaccination against HPV, the main preventive measure. The primary aim of the practical part was to find out the level of HPV awareness among girls and boys and diseases caused by HPV. The secondary aim of the practical part was to find out opinions on HPV vaccination and determine the rate of vaccination against HPV among young people in Slovakia. METHODS The method of quantitative research taken in the form of a questionnaire was used in the diploma thesis. The survey was realized at two secondary schools in Svidník, Slovakia. The age range of respondents was 15-20 years. The data...

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Samples of excrements for parasitic investigation was taken away in 16 breeding, when it was about 3 breeding - when animals were been regularly milking, 11 breeding of fatcattle, one breeding of wisents and one breeding of aurochs. It was been observing the influence of technology of breeding, when it was about ecology breeding. Utility type of fatcattle was been permanently grazing, milk animal was 6 months grazing and 6 months stabled. Hobby breeding of wisents and aurochs was been breeded whole year on the pasture. According the lokality, where the breeding is, was noticed the altitude. Statistic analysis proved that the decrease of prevalence kryptosporids infection was in the straight relationship with the increase of altitude. The most infected was herds breeded to the 500 metres altitude. Ossurrence of endoparasites was observed on the dependence of seasonal occurrence. The most low prevalence of parasites infections was found out at miking animals, so that means stabled animals and grazing. Statistic analysis proved animals breeded in system of whole year grazing are statistical to much more infected by parasites than animals breed like technology combined grazing and stabling. Pursuance of occurence and risk of infection of individual species of parasites were consensus statistical comparison found out that animal whole year grazing are 7,25× frequently infected by fluke of Paramphistomum genus (?2=16,4; d.f.=1; P<0,001). In the comparison was no found out diference beetwen occurrence of cocsids genus Eimeria, infusorian of Buxtonella genus, nematode Trichostrongylidea family and fluke Fasciola hepatica species in the dependence of cattle breeding technology. In the breeding was used anti-parasites medicamets IVOMEC SUPER and HELMIGAL. Pursuance of detailed analysis I grew up the end that application of anti-parasites medicamets had no influence on occurrence and prevalence of Fasciola hepatica fluke s pecies and pulmonary nemathods Dictyocaulus genus. On the contrary animals - which weren´t cured anti-parasites medicamets ? were 4,85× frequently inficated by gastrointestinal nemathods (GIN).