Thesis (Ph. D.)--University of Wisconsin--Madison, 1949. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 72-75).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1951. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 101-105).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1958. / Typescript. Abstracted in Dissertation abstracts, v. 18 (1958) no. 3, p. 752-753. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 93-97).

Thesis (M.S.)--Oregon State University, 1983. / Typescript (photocopy). Includes bibliographical references (leaves 29-33). Also available on the World Wide Web.

No description available.

Thesis (M.S. in zoology)--Washington State University, May 2007. / Includes bibliographical references (p. 17-23).

An investigation was designed to determine the effect of dusts and sprays of 2,4-D on growth, yield, enzyme activity, photosynthesis, respiration and photophosphorylation of bush bean plants. Beans (Phaseolus vulgaris, var. Top Crop) were grown under growth room conditions in five-inch tin cans filled with soil, and in six inch plastic pots filled with vermiculite. Vermiculite-grown beans were irrigated with a modified Shive's three-salt nutrient solution. Treatment unit of 8 to 10 plants was replicated 4 to 6 times. Dusts containing 2,4-D, NCL, NH4•2,4-D and minerals at a rate of 8 pounds per acre were applied to the foliage of plants when 13 or 14 days old. Sprays containing NH4•2,4-D were applied to the plants until run off. The soil-grown beans treated with 2,4-D in dust form were harvested when 8 weeks old. Growth (based on tops) and yield (based on pods) were then determined, and enzyme activity was measured 2, 5, 7, 11, and 14 days after treatment. The vermiculite-grown beans were treated with both dusts and sprays of 2,4-D. Growth and rates of photosynthesis and respiration were determined at one-week intervals for six consecutive weeks after treatment. Yield was determined by collecting pods at one-week intervals for three weeks starting at the fourth week after treatment. The gas exchange method was used for the determination of photosynthesis and respiration. Effects of NCL in dust form and NH4•2,4-D in spray form on chlorophyll content and photophosphorylation 7 days after treatment were studied. The results showed that dusts of 2,4-D significantly increased the number of pods, whereas sprays of NH4•2,4-D did not. NCL 0.12 and NH4•2,4-D 1.0 ppm increased seed yield by 18.52% and 12.66% over the controls. With the exception of NCL 0.12, all the 2,4-D treatments in dust form failed to show a significant effect on growth and yield. Sprays of NH^*2,4-D at 5 ppm significantly inhibited growth and yield. A higher dry weight and percentage dry weight were obtained in dust of 2,4-D treated vermiculite-grown beans. Beans dusted with NCL 0.12 showed a significant increase in fresh weight and dry weight of tops, and fresh weight of pods. The increases were 41.73%, 48.84% and 28.30% over the controls. Application of NCL 0.12 and 2,4-D 0.12 resulted in a significant stimulation of catalase activity in bean leaves but inhibited catalase activity in bean stems in 14 days. In the case of phosphatase, NCL 0.12 caused an inhibition in the enzyme activity of both leaves and stem in 5 days and a stimulation in 14 days. NCL 0.06 treated plants showed a lower catalase activity in 2 days and higher activity in 5, 7, and 14 days. Phosphorylase activity was stimulated in 2 days but was inhibited in 5 days. Minerals accelerated phosphorylase activity in 2 and 5 days. NCL dusts and NH4•2,4-D sprays caused a stimulation in the rate of photosynthesis one week after treatment. This was followed by an inhibition during the period from the second to sixth week after treatment. A higher rate of respiration was found in NH4•2,4-D treated plants whereas in the case of NCL treated plants a higher rate of respiration at the first and sixth week and a lower rate at the second to fifth, week were obtained. Photophosphorylation in plants treated with NCL dusts and NH4•2,4-D sprays was significantly higher than that in untreated plants except that NCL dust showed an uncoupling of phosphate uptake in noncyclic photophosphorylation. / Science, Faculty of / Botany, Department of / Graduate

A study was carried out to gain information, which could be used to describe the mechanism whereby 2,4-D affects the growth of intact plants. Fourteen-day-old bean plants (Phaseolus vulgaris) growing in a phosphate-free or complete nutrient solutioi were sprayed to drip with 5 or 50 ppm 2,4-D. At various times up to 70 hours after spraying, the roots were immersed in a complete nutrient solution containing P³². Following an incorporation period of 2-9 hours, the plants were harvested, and separated into roots, stems plus petioles, and leaves. Acid soluble activity, acid insoluble activity, and the distribution of activity within the soluble fraction as revealed by paper chromatography were determined for each organ. In general the treatments had relatively small effects on phosphorus uptake, translocation, or incorporation into organic compounds. Initial increases in the rate of P³² uptake were not maintained and were followed by the same or less uptake as controls. The rate of upward translocation was reduced and less affected by the age of-the plants, as compared to controls, after treatment with 5 ppm 2,4-D. The higher concentration did not alter the distribution of activity among the organs. While the lower level did not change the rate of incorporation into the total acid insoluble fraction of the plant, the growth-inhibitory level reduced the activity in this fraction by 14-22%. At 5 ppm there was a trend toward more incorporation into insoluble compounds in the roots and less in the leaves, but no consistent change in the stems. The composition of the soluble fractions showed no uniform variations from controls at either concentration. The results indicated a pattern whereby the balance in P³² distribution between the leaves and roots was upset in favor of the roots by foliar application of 5 ppm 2,4-D, but not 50 ppm. Both stimulatory and inhibitory levels of 2,4-D produced responses which appear to be related to the synthesis of acid insoluble compounds in the roots and leaves. The major portion of the insoluble activity in these tissues was also ethanol insoluble and is believed to be RNA. Actigrapb scans of picric acid chromatograms indicated more incorporation of activity into organic compounds (nucleotides and sugar phosphates) in the leaf and stem tissues than in the roots. Plants grown in complete nutrient prior to exposure to P³² , took up, translocated, and retained less phosphate than those grown in phosphate-free nutrient,. Also, incorporation into soluble organic compounds was suppressed. The only radioactive compound in xylem exudates from plants 10 hours after their initial exposure to P³² was orthophosphate. / Science, Faculty of / Botany, Department of / Graduate

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