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Vision studies and visual acuity investigations are an ongoing and progressive field in veterinary ophthalmology. These independent studies all help to contribute to a combined and collective knowledge in our understanding of this truly complex matter. Understanding retinal morphology and physiology is an integral factor in piecing together overall function of the eye. Many of these studies have been done in both medical and veterinary ophthalmology using behavioural factors, electrophysiology, special staining and scanning techniques on a histological level. In the veterinary field many species have been studied pointing out similarities or differences among them. This study hopes to contribute to the understanding of the retinal ultrastructure of the African buffalo (Syncerus caffer). Twenty-five pairs of African buffalo eyes were obtained, but only forty-eight eyes were included in this investigation. The globes of one donor appeared to have chronic intraocular disease and were phthisical. Since this is a descriptive study of normal anatomy and function, these eyes were excluded. Globe dimensions were recorded and statistically analysed, revealing an average horizontal diameter of 32.91mm and a vertical diameter of 33.04mm. The median age of the donor group was 4 years with Using scanning electron microscopy it was established that African buffalo retinas, like other domestic species, have a specialised region a few millimetres dorsolateral to the optic disc, synonymous to the well described area centralis. In this region a higher concentration of cones is found as opposed to other rod-rich regions. In a concurrent investigation, the contralateral globes were processed for immunohistochemical antibody staining. Colour specific anti-bodies were used to identify the cone population present in the African buffalo retina. The conclusion of this investigation reveals that this species like other domestic animals has dichromatic colour vision, recognising short and medium to long colour wavelengths. / Dissertation (MMedVet)--University of Pretoria, 2013. / gm2014 / Companion Animal Clinical Studies / unrestricted

This work is based on Shafer's Dichromatic Reflection Model as applied to color image formation. The color spaces RGB, XYZ, CIELAB, CIELUV, rgb, l1l2l3, and the new h1h2h3 color space are discussed from this perspective. Two color similarity measures are studied: the Euclidean distance and the vector angle. The work in this thesis is motivated from a practical point of view by several shortcomings of current methods. The first problem is the inability of all known methods to properly segment objects from the background without interference from object shadows and highlights. The second shortcoming is the non-examination of the vector angle as a distance measure that is capable of directly evaluating hue similarity without considering intensity especially in RGB. Finally, there is inadequate research on the combination of hue- and intensity-based similarity measures to improve color similarity calculations given the advantages of each color distance measure. These distance measures were used for two image understanding tasks: edge detection, and one strategy for color image segmentation, namely color clustering. Edge detection algorithms using Euclidean distance and vector angle similarity measures as well as their combinations were examined. The list of algorithms is comprised of the modified Roberts operator, the Sobel operator, the Canny operator, the vector gradient operator, and the 3x3 difference vector operator. Pratt's Figure of Merit is used for a quantitative comparison of edge detection results. Color clustering was examined using the k-means (based on the Euclidean distance) and Mixture of Principal Components (based on the vector angle) algorithms. A new quantitative image segmentation evaluation procedure is introduced to assess the performance of both algorithms. Quantitative and qualitative results on many color images (artificial, staged scenes and natural scene images) indicate good edge detection performance using a vector version of the Sobel operator on the h1h2h3 color space. The results using combined hue- and intensity-based difference measures show a slight improvement qualitatively and over using each measure independently in RGB. Quantitative and qualitative results for image segmentation on the same set of images suggest that the best image segmentation results are obtained using the Mixture of Principal Components algorithm on the RGB, XYZ and rgb color spaces. Finally, poor color clustering results in the h1h2h3 color space suggest that some assumptions in deriving a simplified version of the Dichromatic Reflectance Model might have been violated.

This work is based on Shafer's Dichromatic Reflection Model as applied to color image formation. The color spaces RGB, XYZ, CIELAB, CIELUV, rgb, l1l2l3, and the new h1h2h3 color space are discussed from this perspective. Two color similarity measures are studied: the Euclidean distance and the vector angle. The work in this thesis is motivated from a practical point of view by several shortcomings of current methods. The first problem is the inability of all known methods to properly segment objects from the background without interference from object shadows and highlights. The second shortcoming is the non-examination of the vector angle as a distance measure that is capable of directly evaluating hue similarity without considering intensity especially in RGB. Finally, there is inadequate research on the combination of hue- and intensity-based similarity measures to improve color similarity calculations given the advantages of each color distance measure. These distance measures were used for two image understanding tasks: edge detection, and one strategy for color image segmentation, namely color clustering. Edge detection algorithms using Euclidean distance and vector angle similarity measures as well as their combinations were examined. The list of algorithms is comprised of the modified Roberts operator, the Sobel operator, the Canny operator, the vector gradient operator, and the 3x3 difference vector operator. Pratt's Figure of Merit is used for a quantitative comparison of edge detection results. Color clustering was examined using the k-means (based on the Euclidean distance) and Mixture of Principal Components (based on the vector angle) algorithms. A new quantitative image segmentation evaluation procedure is introduced to assess the performance of both algorithms. Quantitative and qualitative results on many color images (artificial, staged scenes and natural scene images) indicate good edge detection performance using a vector version of the Sobel operator on the h1h2h3 color space. The results using combined hue- and intensity-based difference measures show a slight improvement qualitatively and over using each measure independently in RGB. Quantitative and qualitative results for image segmentation on the same set of images suggest that the best image segmentation results are obtained using the Mixture of Principal Components algorithm on the RGB, XYZ and rgb color spaces. Finally, poor color clustering results in the h1h2h3 color space suggest that some assumptions in deriving a simplified version of the Dichromatic Reflectance Model might have been violated.

Introduction Colour codes are used extensively in railways to convey specific information governing movement of trains and equipment on the track. One such task is the railway traffic control display that uses colour coded video display terminals (VDTs) to convey information of the signal status, train movements and track status to the railway dispatcher. Because individuals with colour vision deficiencies (colour-defectives) may have problems with these colour-related tasks, questions were raised about the suitability of colour vision defectives to work as railway dispatchers. In order to answer that, a VDT based Dispatch Colour Vision Test based on the actual railway traffic display was developed previously. Purpose The main purpose of this thesis is to establish the pass/fail scores and repeatability of the VDT based Dispatch Colour Vision Test that resulted from the previous work. Secondly, the study will also examine whether clinical colour vision tests can predict the performance on the practical task. Methods The Dispatch colour vision test was divided into three parts based on the colour sets that the dispatcher had to recognize. The testing computer system used the the same RGB colour settings, graphics card and monitor as in railway dispatch centres. Subjects viewed the display colours and entered their responses by using a mouse. One hundred colour-normals and fifty two colour-defectives participated in the initial session. The test was repeated approximately after 10 days. Ninety three colour-normals (93%) and 44 (85%) colour-defectives participated in the second session. The total number of errors and time to complete the test was recorded. Results Pass/Fail on the VDT Dispatch colour vision test was based on colour-normal errors. Ignoring orange-red errors, two errors were allowed in the first session and one error was allowed in the second session. Based on this criterion, 42% of colour vision defectives could perform as well as colour normal subjects. The kappa coefficient of agreement between the sessions for the colour-defectives was 0.85. Detailed analysis between the colour differences and the errors showed only a weak correlation between the two. However, the general trend was that colour-defectives made more errors on colours that were near or along the same lines of confusions and the colours were nearly equal in luminance. Nevertheless, the interaction between luminance and location with respect to the lines of confusion was not easy to interpret. The time to complete the task for the colour-defectives who passed the test took 14% longer than colour-normals and colour-defectives who failed took 30% longer than colour-normals. All groups showed a similar learning effect with an 18% reduction in mean times to complete the task at the second session. There was no significant correlation between the number of errors and time to complete or the clinical tests and completion times for any of the groups. Clinical colour vision tests have limited value in predicting performance of colour-defectives on the Dispatch test. Logistic analysis results showed that the Farnsworth D-15 along with the Nagel was the best predictor of the VDT Dispatch colour test pass/fail results. However, these results were similar to using the Farnsworth D-15 test alone. Ninety-five percent of the individuals who failed the Farnsworth D-15 also failed the Dispatch test. However, approximately 25% of the individuals who passed the Farnsworth D-15 failed the VDT Dispatch colour test which is an unacceptable false negative rate. These results indicate the Farnsworth D-15 can only be used to predict who is likely to fail the dispatch test. Conclusions Forty two percent of colour vision defectives could perform as well as colour-normals in identifying VDT railway display colours and time to complete the task. Clinical colour vision tests were inadequate predictors of performance in practical task, overall. However, the Farnsworth D-15 was a very good predictor of who would fail the VDT Dispatch test. Hence a practical VDT Dispatch test may be needed to test individuals who would want to work as railway dispatchers.

Introduction Colour codes are used extensively in railways to convey specific information governing movement of trains and equipment on the track. One such task is the railway traffic control display that uses colour coded video display terminals (VDTs) to convey information of the signal status, train movements and track status to the railway dispatcher. Because individuals with colour vision deficiencies (colour-defectives) may have problems with these colour-related tasks, questions were raised about the suitability of colour vision defectives to work as railway dispatchers. In order to answer that, a VDT based Dispatch Colour Vision Test based on the actual railway traffic display was developed previously. Purpose The main purpose of this thesis is to establish the pass/fail scores and repeatability of the VDT based Dispatch Colour Vision Test that resulted from the previous work. Secondly, the study will also examine whether clinical colour vision tests can predict the performance on the practical task. Methods The Dispatch colour vision test was divided into three parts based on the colour sets that the dispatcher had to recognize. The testing computer system used the the same RGB colour settings, graphics card and monitor as in railway dispatch centres. Subjects viewed the display colours and entered their responses by using a mouse. One hundred colour-normals and fifty two colour-defectives participated in the initial session. The test was repeated approximately after 10 days. Ninety three colour-normals (93%) and 44 (85%) colour-defectives participated in the second session. The total number of errors and time to complete the test was recorded. Results Pass/Fail on the VDT Dispatch colour vision test was based on colour-normal errors. Ignoring orange-red errors, two errors were allowed in the first session and one error was allowed in the second session. Based on this criterion, 42% of colour vision defectives could perform as well as colour normal subjects. The kappa coefficient of agreement between the sessions for the colour-defectives was 0.85. Detailed analysis between the colour differences and the errors showed only a weak correlation between the two. However, the general trend was that colour-defectives made more errors on colours that were near or along the same lines of confusions and the colours were nearly equal in luminance. Nevertheless, the interaction between luminance and location with respect to the lines of confusion was not easy to interpret. The time to complete the task for the colour-defectives who passed the test took 14% longer than colour-normals and colour-defectives who failed took 30% longer than colour-normals. All groups showed a similar learning effect with an 18% reduction in mean times to complete the task at the second session. There was no significant correlation between the number of errors and time to complete or the clinical tests and completion times for any of the groups. Clinical colour vision tests have limited value in predicting performance of colour-defectives on the Dispatch test. Logistic analysis results showed that the Farnsworth D-15 along with the Nagel was the best predictor of the VDT Dispatch colour test pass/fail results. However, these results were similar to using the Farnsworth D-15 test alone. Ninety-five percent of the individuals who failed the Farnsworth D-15 also failed the Dispatch test. However, approximately 25% of the individuals who passed the Farnsworth D-15 failed the VDT Dispatch colour test which is an unacceptable false negative rate. These results indicate the Farnsworth D-15 can only be used to predict who is likely to fail the dispatch test. Conclusions Forty two percent of colour vision defectives could perform as well as colour-normals in identifying VDT railway display colours and time to complete the task. Clinical colour vision tests were inadequate predictors of performance in practical task, overall. However, the Farnsworth D-15 was a very good predictor of who would fail the VDT Dispatch test. Hence a practical VDT Dispatch test may be needed to test individuals who would want to work as railway dispatchers.