CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / Empresa Brasileira de Pesquisa AgropecuÃria / O objetivo desta pesquisa foi desenvolver formulaÃÃes de sucos tropicais mistos, baseando-se em dados da Dieta MediterrÃnea (DM), utilizando ferramentas de otimizaÃÃo de processos, para avaliar e quantificar possÃveis efeitos aditivo, sinergÃstico e antagÃnico entre as variÃveis, e avaliar o perfil funcional in vitro e in vivo dos sucos. Foi utilizado um planejamento estatÃstico do tipo fracionado para seleÃÃo das variÃveis (P<0,10), seguido de um delineamento composto central rotacional (DCCR) 25 com P<0,05. As variÃveis independentes foram Ãs concentraÃÃes das polpas de frutas (%) das seis espÃcies de frutas tropicais (camu-camu, acerola, caju, cajÃ, aÃaà e manga) e como variÃveis dependentes a capacidade antioxidante total (TAC) atravÃs do mÃtodo ABTS, polifenÃis totais (TP), Ãcido ascÃrbico e aceitaÃÃo sensorial. Para os ensaios in vivo foram utilizadas duas formulaÃÃes de suco tropical misto: formulaÃÃo A (suco tropical misto de acerola, abacaxi, aÃaÃ, caju, cajà e camu-camu) e formulaÃÃo B (suco tropical misto de acerola, abacaxi, aÃaà e cajÃ), com suas diferentes porcentagens de polpa (%). Ratos machos da linhagem Wistar, recÃm-desmamados foram distribuÃdos em 7 grupos, sendo controle (Ãgua), e seis grupos de animais tratados por gavagem com a reconstituiÃÃo em Ãgua das formulaÃÃes dos sucos liofilizadas nas concentraÃÃes: 100, 200 ou 400mg/kg de peso corpÃreo, durante 30 dias. Foram avaliados os Ãndices nutricionais de consumo de raÃÃo e ganho de peso; anÃlises bioquÃmicas: glicose, triglicerÃdeos, colesterol total, HDL (High Density Lipoprotein), alanina aminotransferase (ALT) e aspartato aminotransferase (AST); peroxidaÃÃo lipÃdica do soro e fÃgado, pelo mÃtodo TBARS (substÃncias reativas ao Ãcido tiobarbitÃrico) e atividade das enzimas antioxidantes, catalase (CAT), superÃxido dismutase (SOD) e glutationa peroxidase (GSH-Px), nos eritrÃcitos, e fÃgado. A partir da anÃlise dos planejamentos estatÃsticos, o camu-camu, a acerola e o aÃaà foram os principais fatores que influenciaram o potencial antioxidante das formulaÃÃes, e o cajà mostrou um efeito positivo sobre a aceitaÃÃo sensorial dos sucos tropicais. Observou-se um efeito antagÃnico entre acerola e camu-camu para a resposta TAC. A formulaÃÃo otimizada foi composta por 20% acerola, 10% de camu-camu, 10% de cajÃ, 10% caju e 10% de aÃaÃ, que correspondeu a um resultado de 155,46 mg.100 g-1 de Ãcido ascÃrbico, 103,01 mg de GAE.100 g-1 para TP, 10,27 ÂM Trolox g-1 para TAC e aproximadamente 6,1 de aceitaÃÃo sensorial. Os grupos tratados com as formulaÃÃes de sucos mistos nÃo apresentaram diferenÃa significativa em relaÃÃo aos Ãndices nutricionais e parÃmetros bioquÃmicos, incluindo a atividade das enzimas ALT e AST, indicando que as formulaÃÃes nÃo ocasionaram danos hepÃticos aos animais. Os resultados demonstraram que a atividade das enzimas SOD e CAT no fÃgado (FA200), e GSH-Px nos eritrÃcitos (FB400), e TBARS no soro e fÃgado (FB100, FA400, FB200, FB400) foi significantemente reduzida nos grupos tratados com os sucos de frutas, quando comparados com o grupo controle, enquanto que o HDL-c aumentou (FB400). Os resultados in vitro e in vivo sugerem que o consumo dos sucos tropicais mistos desenvolvidos neste trabalho foi eficaz na defesa antioxidante endÃgena, sugerindo efetivamente que os sucos de frutas tropicais podem ter significativa relevÃncia para efeitos benÃficos a saÃde. / The aim of this research was to optimize the formulation of mixed tropical juices, based on research into the Mediterranean Dietâ (MD), using a statistical design of fractional type for variable selection (P<0.10), followed by a planning type DCCR (Delineation central composite rotational) 25 with P<0.05, and response surface methodology (RSM), which it was possible to assess. Moreover this investigation proposed to quantify possible additive effects, synergisms and antagonisms between variables, and to evaluate in vitro and in vivo profile of functional the juices. We used six species of tropical fruits (camu-camu, acerola, cashew, yellow mombin, acai and mango). The dependent variables were analyzed: total antioxidant capacity (TAC) using ABTS method, total polyphenols (TP), ascorbic acid and sensory acceptance. The independent variables were the concentrations of fruit pulp (%). For evaluate the in vivo assays were used two formulations of optimized mixed tropical fruit: The formulation (mixed tropical acerola juice, pineapple, acai, cashew, yellow mombin and camu-camu) and formulation B (mixed tropical acerola juice, pineapple, acai and yellow mombin), with different pulp proportions (%) and weaned rats that were divided in 7 groups: control (water), six groups of animals treated by gavage in water to reconstitute lyophilized juice formulations at concentrations of 100, 200 or 400mg/kg for 30 days. The followed analyzes were performed: The nutritional indices of feed intake and weight gain; biochemical analyzes of glucose, triglycerides, total cholesterol, HDL (High Density Lipoprotein), alanine aminotransferase (ALT) and aspartate aminotransferase (AST), serum lipid peroxidation and liver method TBARS (thiobarbituric acid reactive substances) and activities of antioxidant enzymes, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in erythrocytes and liver. Concerning to the statistical planning and MSR, camu-camu, acerola and acai were the main factors that influenced the antioxidant potential of the juice, and yellow mombin showed a positive effect on sensory acceptability of tropical juice. There was an antagonistic effect between acerola and camu-camu in regarding to TAC. The optimal formulation was composed of 20% acerola, 10% camu-camu, 10% yellow mombin, 10% cashew and 10% acai, which corresponding a result of 155,46 mg.100g-1 ascorbic acid, 103,01 mg GAE. 100 g-1 TP, 10,27 ÂM Trolox g-1 for TAC and sensory acceptance of approximately 6.1. The groups treated with the formulations of mixed juices showed no statistical significant difference in relation to nutritional indices and biochemical parameters, including the activity of the enzymes ALT and AST, indicating that the formulations did not cause liver damage these animals. The results showed that the SOD activity and CAT in the liver (FA200) and GSH-Px in erythrocytes (FB400) and in serum and liver TBARS (FB100, FA400, FB200, FB400) were efficiently reduced in the groups treated with the fruit juices, when compared with the control group, while HDL-c increased (FB400). In conclusion, daily consumption of 200mL of optimized formulation is responsible for approximately 50% of the recommended amount of antioxidants in the Mediterranean diet pattern, therefore, a rich source for these bioactive compounds. The results of in vitro and in vivo studies suggest that consumption of tropical juices mixed evaluated was effective in endogenous antioxidant defense, and effectively suggest that the tropical fruit juices may have significant relevance to the health beneficial effects.