The aim of the present study was to investigate how dispositional resistance to change (RTC) and neuroticism could predict shift work tolerance (SWT) over a period of six months. Electronic questionnaire were completed by 74 shift workers employed in Trondheim municipality in January 2013 (T1) and in June 2013 (T2). The results showed that age, gender, neuroticism, and RTC were related to SWT. Age at T1 predicted better SWT at T2, while male gender predicted worse SWT at T2. RTC at T1 predicted better SWT at T2, while neuroticism at T1 predicted worse SWT at T2. The findings suggest that individual differences, especially neuroticism, can predict SWT over a period of six months.