Four sibling species of the Drosophila melanogaster species subgroup (D. melanogaster, D. simulans, D. sechellia and D. mauritiana) were used to study genetic variation at the protein level by improved two-dimensional (2DE) gel electrophoresis. Three of the species, D. simulans, D. sechellia and D. mauritiana, are chromosomally homosequential, but their phylogenetic relationship to each other is a subject of controversy. Eight tissues representing adult and larval (developmental stage) and reproductive and non-reproductive tissues were analysed for protein variation. The tissues used were as follows: larval testis, brain, haemolymph, wing disc, and adult testis, accessory gland, male and female brain. Close to 400 protein spots were detected per tissue using this sensitive method. Each tissue was compared between species for protein variation. Protein variation was measured on the basis of qualitative differences (presence/ absence) of the protein spots in six pairwise species comparisons and four-way comparisons. Different levels of protein variation were detected for the same tissues in different species comparisons and each tissue showed different levels of variation in the same species. Different tissue proteins seemed to evolve independently in different species. But there was no evidence of any trend or pattern to show that either larval or adult patterns are more diverged than the other. The variation between tissues rather than developmental stages appears to be the major determinat of the level of divergence. The reproductive tract (testis and accessory gland) proteins showed more variation among species comparison than the non-reproductive tissue proteins. Among the four species, D. melanogaster testis proteins, both larval and adult, showed the maximum divergence. From the results it seems that there is a correlation between the level of reproductive tract protein divergence and the degree of reproductive isolation observed among these species. Among the other three closely related species, the levels of divergence of the reproductive tract proteins are similar, but lower than that of D. melanogaster. The phylogenetic relationship based on 2DE protein divergence showed D. simulans to be closer to D. mauritiana than to D. sechellia. / Thesis / Master of Science (MS)

Microscopic simulation traffic models are widely used by transportation researchers and practitioners to evaluate and plan for transportation facilities. The intent of these models is to estimate the second-by-second vehicle movements and interactions on such facilities. Due to constraints related to time, budget, and availability of data, these models are typically designed in such a way where the microscopic output is viewed on the macroscopic level. Inherently, this can leave uncertainty to how the model estimates the individual interactions between vehicles on the microscopic level. This thesis utilizes three microsimulation models, INTEGRATION, VISSIM, and CORSIM, to investigate the lane changing behavior as vehicles approach a freeway diverge area. The count of lane changes, lane use distribution, and visual inspection of the simulated lane changing behavior was compared to video data collected at two freeway diverge areas on U.S. 460 in the vicinity of Blacksburg, Virginia during both off-peak and peak periods. It was observed that all three models generally overestimated the number of lane changes near the diverge areas compared to field observations. By modifying the models' lane changing logic, the models were able to closely match field observations in one of the four scenarios. It was found that microsimulation models accurately estimated the lane use distribution. In addition, the INTEGRATION lane use distribution results were found to be more consistent when compared to observed lane use distribution than either VISSIM or CORSIM. / Master of Science

The real phenomena of sea surface are interacting by much kind of different waves.In these phenomena, the gravity standing wave is most important. The gravity standing wave is formed by two progressive waves that possessing same properties but opposite directions. Gravity standing wave can also form by the interaction of a progressive wave with it¡¦s totally reflection wave. Because of the nonlinear interaction of two waves, there must result a double-crest phenomenon of wave pressure. It is dangerous for the navigation of ship when the double-crest appears, and it¡¦s certainly to take the phenomenon into consideration when we are going to design a jetty. In this paper, it bases on the reference of Chen (1989, 1990) who obtained a third-order approximation of two-wave trains interactions in a uniform depth wave field. Further, in this paper, it checks the result that the double-crest phenomenon is formed by which one of these nonlinear terms. Furthermore, research the influences of wave steepness, wave periods, and water depths these factors will cause the crest diverges is also the purpose.

A riparian ecosystem is the area between the aquatic and terrestrial setting of a stream, and serves as a corridor and habitat for birds. Several riparian ecosystems are located in urban environments, and three main riparian corridors are located in Potchefstroom. They are the Mooi River, Wasgoed Spruit, and Spitskop Spruit, which encompass a wide range of different vegetation types and anthropogenic factors. Therefore, different habitat types for birds occur along the riparian corridors of Potchefstroom. Factors such as food and water availability, nesting sites, competition, predation, learning, presence of other species, and those species that are able to adapt to environmental changes influence the avian diversity and communities along riparian corridors. The hypothesis is that bird variables along the riparian corridors in Potchefstroom are affected by vegetation, anthropogenic, and seasonal influences. To investigate these affects, two secondary objectives were formulated. The first was to characterise riparian avian habitats (CAHs) according to vegetation and anthropogenic factors, and the second was to identify temporal and spatial changes in avian variables. The three streams were divided into 79 consecutive transects, each 300 m long. The study area consisted of: 17 transects along Spitskop Spruit, 12 along Wasgoed Spruit and 50 along the Mooi River. Bird observations were conducted monthly from June 2006 to June 2007. Birds that were observed with a perpendicular distance ≤ 30 meters towards the streams were included in the results. The bird species that were observed were also classified into different nesting and feeding guilds. Environmental data recorded included: vegetation structure (estimated cover percentages and height classes of trees, shrubs, grasses, herbs, sedges, and reeds), anthropogenic structures (estimated cover percentages of roads, footpaths, bridges, electrical pylons, houses, and drainage pipes), and the presence of informal settlers along each transect (the mean number of people and the space they occupy). Vegetation was monitored in summer– (February 2007 until April 2007) and winter months (June 2007 until August 2007). The anthropogenic structures and the presence of informal settlers (anthropogenic factors) were monitored simultaneously with the bird counts. Transect-time profiles were drawn for the four parameters, which differed on spatial and time scales. Multivariate analyses included non-metric multidimensional scaling (NMS), cluster analysis, and indicator species analysis. Cluster analyses and NMS bi-plots were used to define characterised avian habitats (CAHs). Two types of CAHs were characterised: Summer CAHs (summer vegetation and anthropogenic factors) and Anthropogenically CAHs (Anthropogenic factors alone). Bird species were then ordinated with the summer and anthropogenically CAHs on NMS successional vector graphs. The successional vectors illustrate the avian community trajectories of the different CAHs. Indicator species analyses were performed to describe associations between the bird species and the summer and anthropogenically CAHs. The summer and anthropogenic CAHs that were characterised had different avian community trajectories and different species were associated with these CAHs. Different levels in avian diversity appeared among these CAHs, and convergence and divergence in communities appeared among these CAHs. Birds also selected their habitats according to feeding and nesting behaviours. Consequently, it can be deduced that environmental factors such as vegetation structures and anthropogenic factors, as well as seasonality, had an effect on the distribution of birds along the riparian corridors of Potchefstroom. / Thesis (Master of Environmental Sciences)--North-West University, Potchefstroom Campus, 2013

A riparian ecosystem is the area between the aquatic and terrestrial setting of a stream, and serves as a corridor and habitat for birds. Several riparian ecosystems are located in urban environments, and three main riparian corridors are located in Potchefstroom. They are the Mooi River, Wasgoed Spruit, and Spitskop Spruit, which encompass a wide range of different vegetation types and anthropogenic factors. Therefore, different habitat types for birds occur along the riparian corridors of Potchefstroom. Factors such as food and water availability, nesting sites, competition, predation, learning, presence of other species, and those species that are able to adapt to environmental changes influence the avian diversity and communities along riparian corridors. The hypothesis is that bird variables along the riparian corridors in Potchefstroom are affected by vegetation, anthropogenic, and seasonal influences. To investigate these affects, two secondary objectives were formulated. The first was to characterise riparian avian habitats (CAHs) according to vegetation and anthropogenic factors, and the second was to identify temporal and spatial changes in avian variables. The three streams were divided into 79 consecutive transects, each 300 m long. The study area consisted of: 17 transects along Spitskop Spruit, 12 along Wasgoed Spruit and 50 along the Mooi River. Bird observations were conducted monthly from June 2006 to June 2007. Birds that were observed with a perpendicular distance ≤ 30 meters towards the streams were included in the results. The bird species that were observed were also classified into different nesting and feeding guilds. Environmental data recorded included: vegetation structure (estimated cover percentages and height classes of trees, shrubs, grasses, herbs, sedges, and reeds), anthropogenic structures (estimated cover percentages of roads, footpaths, bridges, electrical pylons, houses, and drainage pipes), and the presence of informal settlers along each transect (the mean number of people and the space they occupy). Vegetation was monitored in summer– (February 2007 until April 2007) and winter months (June 2007 until August 2007). The anthropogenic structures and the presence of informal settlers (anthropogenic factors) were monitored simultaneously with the bird counts. Transect-time profiles were drawn for the four parameters, which differed on spatial and time scales. Multivariate analyses included non-metric multidimensional scaling (NMS), cluster analysis, and indicator species analysis. Cluster analyses and NMS bi-plots were used to define characterised avian habitats (CAHs). Two types of CAHs were characterised: Summer CAHs (summer vegetation and anthropogenic factors) and Anthropogenically CAHs (Anthropogenic factors alone). Bird species were then ordinated with the summer and anthropogenically CAHs on NMS successional vector graphs. The successional vectors illustrate the avian community trajectories of the different CAHs. Indicator species analyses were performed to describe associations between the bird species and the summer and anthropogenically CAHs. The summer and anthropogenic CAHs that were characterised had different avian community trajectories and different species were associated with these CAHs. Different levels in avian diversity appeared among these CAHs, and convergence and divergence in communities appeared among these CAHs. Birds also selected their habitats according to feeding and nesting behaviours. Consequently, it can be deduced that environmental factors such as vegetation structures and anthropogenic factors, as well as seasonality, had an effect on the distribution of birds along the riparian corridors of Potchefstroom. / Thesis (Master of Environmental Sciences)--North-West University, Potchefstroom Campus, 2013