The thesis deals with the diversity of weed vegetation and a fixation the presence of expansive species on chosen part of railway between Brno-main station and Řečkovice station. The observing of vegetation was made on railway embankment, around the railway embankment and between rails. Locations have been observing in July and August 2011 and 2014. The observation results were statistically evaluated by DCA and CCA analysis. On the chosen part was found 50 species of plants in total. The most coverage had Urtica dioica L., Solidago canadensis L., Artemisia vulgaris L. The most common species between the rails were: Euphorbia cyparissias L., Artemisia vulgaris L, Achillea millefolium L. On the railway embankment were the most common these species: Lolium perenne L., Plantago major L., Artemisia vulgaris L. Around the railway embankment were the most commons species: Urtica dioica L., Solidago canadensis L., Artemisia vulgaris L. Key words: