Background: Every year approximately 5 000-10 000 persons in Sweden are diagnosed for Lyme disease. In Europe the incidence is about 65 000 persons per year. Lyme disease is a tick-borne zoonosis, the causative agents – Borrelia burgdorferi-genospecies - are transmitted by Ixodes-ticks (in Sweden primary I.ricinus). Lyme disease is treated with antibiotic-therapy. Though, in 5-15 percent of the cases, post-treatment symptoms can appear and persist for six months, or longer. Today we don´t know the origin of the phenomena. Some patients seek help outside the Swedish borders, and turn to so called Lyme disease-clinics – which, in some cases, institutes long-term antibiotic-treatment. The pharmacist may encounter this particular matter, while carrying out EES-antibiotic-prescriptions, prescribed non-analogous to Swedish guidelines.  Objective: The aim of this study was to investigate the efficacy of doxycycline, with respect to short-, middle-, and long-term Lyme disease-treatment. The intention was also to investigate the pharmacist role in executing EES-antibiotic-prescriptions.  Methods: The study was divided into two parts. The first part was investigating doxycycline, and was based on five scientific articles collected from PubMed. The second part was a review of the pharmacist role in processing EES-antibiotic-prescriptions; information was collected by email correspondence with pharmacy chain stores, authorities and federation of labor unions.  Results: Awareness and protective measures regarding ticks and Lyme disease seems dire. Improved diagnostic methods, uniform interpretation of outcome, standardized laboratory-analysis is of paramount importance. The pharmacist concerns with EES-antibiotic-prescriptions is carrying out the medical prescription and giving medical advice. Doxycycline in 10-14 days Lyme disease-treatment was seen as an alternative, supported by Swedish guidelines. The post-exposure prophylaxis was not a recommended alternative. Regarding doxycycline and long-term Lyme-disease-treatment, it was postulated that additional scientific studies was needed. Conclusions: The tick, vector of Borrelia burgdorferi, will be favoured of recent and upcoming climate changes. In the future to come, we can expect an expansion of the tick-habitats and with it follows the probability of more frequent encounters with the human race. This will most likely contribute to a higher incidence of Lyme disease. Prevention and subject-enlightenment is of need. The pharmacist will in the professional role be exposed to daily moral dilemmas; one of these dilemmas can be the execution of EES-antibiotic-prescriptions, not prescribed accordingly to Swedish guidelines, with respect to Lyme disease. As things stand today, the primary commitment will be medical advice and carrying out the prescription. The post-exposure prophylaxis regarding doxycycline and Lyme disease is not recommended. The 10-14 days doxycycline-cure, regarding some Lyme disease-manifestations, is mostly a preferable alternative today. Prolonged antibiotic-treatment with doxycycline, in respect to post-treatment symptoms, needs to be further evaluated with better diagnostic methods, scientific studies, standardized analysis and more uniform interpretation of results.

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Veronika Ackermannová Supervisor: prof. Radomír Hrdina, MD, CSc. Title of diploma thesis: Topic and systemic treatment of acne vulgaris Acne vulgaris is a skin disease affecting the hair follicles and sebaceous glands. The disease manifests itself by increased sebum production, non-inflammatory (comedones) and inflammatory lesions (papules, pustules, nodules, cysts). It occurs predominantly in adolescents, but may persist into adulthood. It is a multifactorial disease, which is caused by several factors (internal and external stimuli). The major pathogenetic factors include increased sebum production, hyperkeratosis, P. acnes colonization and inflammation present. First, it is necessary to diagnose the type of acne in order to choose the right and effective therapy, because there is not only one type of acne. There are many types and variants of acne, and although they show similar symptoms (affecting the follicles of sebaceous glands), their cause often differs. There is no uniform classification system for acne vulgaris and it varies between authors. Some authors classify acne vulgaris according to severity into mild, moderate and severe, others into comedonic, papulopustular, nodulocystic...