Thesis (Ph.D.)--Aberdeen University, 2009. / Title from web page (viewed on Dec. 1, 2009). Includes bibliographical references.

This thesis is a theological reflection on substance use and misuse. René Girard’s mimetic theory of human Desire is used as a hermeneutic to explicate the formation of self in Western modernity. The role of intoxicative and hallucinogenic substances in Western culture is seen primarily as facilitating the Capitalist template of homo oeconomicus whose central tenets are individualism, autonomy and rationalism. The mind-altering quality of such substances is a means to cope with the existential angst of individualist modes of life and provides, in the milieu of social interaction, an artificial and temporary sense of connectedness with others. Humanity’s ubiquitous search for connectedness and meaning beyond itself is argued as its defining spiritual character. Consequently the addiction recovery principle of sobriety through accountability to a Higher Power – seen as a spiritual principle and practised in recovery programmes based on the twelve-step method of Alcoholics Anonymous – provides the foundation for the empirical aspects of this study. Lifestory narratives of a sample of recovering individuals reveal the spiritual roots of substance misuse as disconnection and isolation from certain significant others (such as parents), or from a normative code of early social settings (such as schools). The role of intoxicative and hallucinogenic substances in providing alternative means of connection and belonging is illustrated through the mimetic patterns in the narrative accounts which substantiate Girard’s reading of human Desire as fundamentally contingent upon the Other. In considering the implications for the Church’s praxis, Girard’s notion of nonrivalistic mimesis is elucidated as an antidote to Capitalist Desire in which the Church unwittingly participates. Hauerwas’ vision of a communal and sacrificial witness is put forward as an alternative template for the Church in its witness and offer of Christocentric relationality whose economy of Love removes the need for mindaltering substances in order to affirm one’s identity.

This thesis applies catFISH, a variant of the standard fluorescence in situ hybridisation technique, to study the neuronal ensembles activated by heroin and cocaine across brain structures involved in motivated behaviour, in the Sprague-Dawley rat. The first chapter reviews the pharmacology of heroin and cocaine, rodent models of drug-related behaviours, and heroin and cocaine's ability to trigger immediate-early gene expression when administered acutely or chronically. It is suggested that the empirical evidence points towards a significant separation between the neuronal systems engaged by the two drugs. The main goal of this thesis was to test whether this separation can be seen within brain areas playing a key role in motivation and reward (e.g. the nucleus accumbens). Since immediate-early genes serve as markers of neuronal activity, and catFISH is a technique which can detect the expression of such genes in response to two separate stimuli, the technique was chosen as the best method to test if heroin and cocaine activate the same neuronal ensembles when administered acutely. The second chapter summarises the methods used across experiments described in following chapters. The third chapter presents an experiment addressing the methodological issues associated with using catFISH to study pharmacological stimuli. The technique was originally used to study the hippocampus and brain activity triggered by stimuli with well-controlled on- and offset (e.g. exposure to a novel environment or discrete cues). Arguably, acute drug injections comprise a stimulus with an on- and offset which can only be controlled indirectly – they depend on the drug dose and route of administration, among other factors. It was shown that acute intravenous injections of heroin and cocaine at doses usually self-administered by animals are suitable stimuli to use with catFISH. Chapter four describes an experiment showing that, across the striatum, the neuronal ensembles activated by an injection of cocaine followed by an injection of heroin overlap significantly less than the neuronal ensembles activated by two consecutive injections of cocaine. This is interpreted as direct evidence for a significant separation between the neuronal pathways activated by heroin vs. cocaine, even in brain areas often considered a common neurobiological substrate for the effects of the two drugs. It must be noted that the magnitude and the nature of this separation depends on the particular part of the striatum and the order in which drugs are administered. Chapter five describes a pilot experiment attempting to incorporate the logic of the catFISH technique into a rodent drug self-administration paradigm. It was found that the rats preferred self-administering heroin over cocaine, and that there was no detectable expression of the homer 1a gene across the striatum in response to acute heroin and cocaine after extended experience with the two drugs. There was also no change from baseline expression of the homer 1a and arc genes in areas of the prefrontal cortex. Finally, chapter six summarises the main findings and the key methodological considerations from all three experiments. As a whole, it is suggested that the experiments in this thesis provide a proof of concept that heroin and cocaine are processed differently by the brain, even within brain areas considered to be common substrates for the reinforcing and addictive properties of the two drugs. Future studies should take this separation into account, as it may have important implications for understanding drug addiction as a whole. The appendices contain representative fluorescence microscopy images of brain tissue analysed for catFISH.

This thesis investigates the phenomenological task of asking what the lived experience of the wounded healer is like for ex-drink/drug-addict Counselling Psychologists working in addiction. The wounded healer is a term that has been circulating in medical and psychotherapy circles for the past 150 years and has been associated with the helping professions and in particular addiction.

Thesis (M.S.)--University of North Carolina at Wilmington, 2005. / Includes bibliographical references (leaves: [28]-31)

Thesis (Ph. D.)--Pardee RAND Graduate School, 2004. / Includes bibliographical references (p. 126-135).

Thesis (Ph. D.)--University of Chicago, Faculty of the School of Social Service Administration, June 2000. / Includes bibliographical references. Also available on the Internet.

Thesis (M.A.)--University of North Carolina at Wilmington, 2005. / Includes bibliographical references (leaves: [58]-66)

Thesis (Ph.D.)--Pardee RAND Graduate School, 2004. / Includes bibliographical references (p. 126-135).

α2 subunit-containing GABAA receptors (α2-GABAARs) are abundantly expressed in the nucleus accumbens (NAc), a region thought to be important in mediating cocaine's reinforcing properties. This thesis develops viral-based RNAi tools in efforts to investigate the functional role of mesoaccumbal α2-GABAARs in mediating cocaine's ability to facilitate conditioned behaviours (i.e. behavioural sensitisation and conditioned reinforcement). RNA interference (RNAi)-mediated knockdown of the α2 subunit expression in the NAc core neither affected appetitive Pavlovian learning nor instrumental learning maintained by the conditioned reinforcer, but blocked cocaine facilitation of conditioned reinforcement. This behavioural phenotype was also observed upon α2 knockdown specifically in NAc core dopamine D2 receptor (D2R)-containing neurons, whereas α2 knockdown in mesoaccumbal D1R-containing neurons reduced the level of discriminated approach during Pavlovian learning. Further, α2 knockdown in the NAc core or shell did not block cocaine-induced sensitisation as previously observed in the constitutive knockouts (Dixon et al., 2010), but the latter increased acute locomotor responses to cocaine. Data presented within this thesis indicate that GABAergic signalling via α2-GABAARs within the NAc is involved in some of the motivation-enhancing properties of cocaine, most likely via interactions with the dopaminergic system.