Diploma thesis of theme: Analysis of Species Composition of Vegetation in Vineyards of Selected Wine-Growing Villages deals with the evaluation of weed species composition in wine-growing villages Hlohovec, Moravský Žižkov, Sudoměřice and Horní Dunajovice. Observations were made in July 2018 and the composition of weed species was evaluated by using the phytocoenological surveys. Recorded data were statistically processed by DCA analysis, followed by RDA analysis and their output was ordination diagrams. 172 species were observed in all vineyards. In the vineyards of the wine-growing village Hlohovec were mainly species: Chenopodium hybridum, Artemisia absinthium, Consolida regalis. In the vineyards of the wine-growing village of Moravský Žižkov were mainly species: Plantago major, Festuca rubra, Carduus acanthoides, There were species in the vineyards of the wine-growing village of Sudoměřice: Festuca pratensis, Daucus carota, Picris hieracioides. In the vineyards of the village of Horní Dunajovice were mainly species: Trifolium repens, Hieracium pilosella, Cirsium arvense.