The variation in community composition among sites is often used to gain insight into the processes of plant community assembly. In this study, we looked for evidence of environmentally and spatially mediated community assembly mechanisms in a temperate forest in eastern North America. To test this, we measured, identified and mapped all woody stems ≥1 cm in diameter at breast height (DBH) in 12 ha of a 20 ha forest plot. We used principal coordinates of neighbor matrices (PCNM) to obtain variables that modelled spatial processes (eg. dispersal, drift) at the community level. Topographic variables (slope, elevation, convexity, aspect) were used to model environmental conditions. Variation partitioning was used to isolate the unique and shared effects of topographic and spatial variables on community composition. We were also interested in studying how associations with the environment change with tree size. For this we assessed the abundance of a subset of focal species in response to topography as well as human disturbance. Species abundance were divided into three stem size classes: small (< 5 cm), medium (≥5 cm and < 15), and big (≥15 cm). We found that topography and space jointly explained 63% of the variation in community composition. This variation was almost entirely spatially structured with the component of pure topography only contributing 1% to the total explained variation. A redundancy analysis showed that slope and elevation were the most important topographic variables structuring the distribution of trees. The focal species had largely independent distributions across the environmental gradients and three of the five species showed within-species differences associated with size class effects on the relationship with topography and human disturbance. The implications of these results are relevant to conservation efforts and suggest that large contiguous areas of heterogeneous environments are essential in maintaining biodiversity. / Thesis / Master of Science (MSc)