Spatiotemporal variation in animal space use is critical for understanding how individual animals respond to changes in resource availability across space and time. My study was aimed to: 1) determine functional responses of habitat selection by eastern wild turkeys (Meleagris gallopavo silvestris) across 7 study sites in Mississippi; and 2) determine the effect of temporal vegetation variation on order-II habitat selection by wild turkeys over 12 years. I developed resource selection functions using radio telemetry location data. Individual-specific coefficients of order-III habitat selection for forest were related inversely to forest availability in meta-regressions. Yearly coefficients of order-II habitat selection for forest were related inversely to the mean normalized difference vegetation index (NDVI) in April, but the coefficients for open fields were related positively to coefficient of variation in the NDVI from March to May. Wild turkeys exhibited functional responses of habitat selection to spatiotemporal forest availability across Mississippi.