An unsupervised classification was applied to continuous biophysical variables in an attempt to delineate ecoregional boundaries in the desert southwest. Output was then compared with ecoregions delineated by the Natural Resources Conservation Service (NRCS), the Environmental Protection Agency (EPA), and the Forest Service at the national level. An attempt was made to use the same biophysical variables for input into the unsupervised classification as was emphasized by the various agencies with their ecoregional classifications at the desert level. Major constraints included data availability at such a large study area, data resolution, and data that were continuous. This eliminated categorical data such as vegetation type, geology type, or soil texture. The aim of the study was to develop a more objective and repeatable approach to identifying self-similar geographic regions.