Objective: To examine the influence of a 6-week training program on the electromechanical delay (EMD) and reaction time of the peroneus longus muscle. Design and Setting: The study was guided by a 2 x 2 factorial design with repeated measures on the time factor. The independent variables for this study were group (training and control) and time (pre- and post-training). Dependent variables for this study were muscle reaction time and electromechanical delay of the peroneus longus muscle. Subjects: Thirty-six healthy, physically active, college-age (21.8 ± 2.3 yr) male and female (M = 14, F = 28, height = 173.7 ± 11.2 cm, weight = 69.1 ± 18.4 kg) subjects were recruited for this study. Subjects had experienced no more than one ankle sprain to either ankle in their life, and had not sprained either ankle in the last year. Subjects were not currently experiencing any lower extremity pathology and had no history of serious injury to either lower extremity. Measurements: The EMD of the peroneus longus was determined by the onset of force contribution after a percutaneous electrical stimulation was administered, as measured by EMG and force plate data. Reaction time was measured after a perturbation during walking. Data was analyzed using two 2 X 2 X 2 ANOVAs. Group (treatment and control) and gender were between treatments factors, and time was a within treatments factor. Results: Upon initial examination, there was a trend in the EMD measurements to show an increase in EMD in the treatment group. However, this lacked statistical significance (F = 2.96, p = 0.0983). Reaction time demonstrated a trend towards a decrease in reaction time in the treatment group, but again, this lacked statistical significance (F = 2.88, p = 0.1025). Effect size for this reaction time was 1.2. Conclusions: The 6-week training program used in this study did not have a significant effect on the reaction time and electromechanical delay of the peroneus longus muscle.