The before-after study is still the most popular method used by traffic engineers and transportation safety analysts for evaluating the effects of an intervention. However, this kind of study may be plagued by important methodological limitations, which could significantly alter the study outcome. They include the regression-to-the-mean (RTM) and site-selection effects. So far, most of the research on these biases has focused on the RTM. Hence, the primary objective of this study consists of presenting a method that can reduce the site-selection bias when an entry criterion is used in before-after studies for continuous (e.g. speed, reaction times, etc.) and count data (e.g. number of crashes, number of fatalities, etc.). The proposed method documented in this research provides a way to adjust the Naive estimator by using the sample data and without relying on the data collected from the control group, since finding enough appropriate sites for the control group is much harder in traffic-safety analyses. In this study, the proposed method, a.k.a. Adjusted method, was compared to commonly used methods in before-after studies. The study results showed that among all methods evaluated, the Naive is the most significantly affected by the selection bias. Using the CG, the ANCOVA, or the EB method based on a control group (EBCG) method can eliminate the site-selection bias, as long as the characteristics of the control group are exactly the same as those for the treatment group. However, control group data that have same characteristics based on a truncated distribution or sample may not be available in practice. Moreover, site-selection bias generated by using a dissimilar control group might be even higher than with using the Naive method. The Adjusted method can partially eliminate site-selection bias even when biased estimators of the mean, variance, and correlation coefficient of a truncated normal distribution are used or are not known with certainty. In addition, three actual datasets were used to evaluate the accuracy of the Adjusted method for estimating site-selection biases for various types of data that have different mean and sample-size values.

Neste trabalho apresenta-se uma revisão da metodologia de experimentos de microarray relativas a sua instalação e análise estatística dos dados obtidos. A seguir, aplica-se essa metodologia na análise de dados de expressão gênica em citros, gerados por um experimento de macroarray, utilizando modelos lineares de efeitos fixos considerando a inclusão ou não de diferentes efeitos e considerando ajustes de modelos para cada gene separadamente e para todos os genes simultaneamente. Os experimentos de macroarray são similares aos experimentos de microarray, porém utilizam um menor número de genes. Em geral, são utilizados devido a restrições econômicas. Devido ao fato de terem sido utilizados poucos arrays no experimento analisado neste trabalho foi utilizada uma abordagem bayesiana empírica que utiliza estimativas de variância mais estáveis e que leva em consideração a correlação entre as repetições do gene dentro do array. Também foi utilizado um método de análise não paramétrico para contornar o problema da falta de normalidade para alguns genes. Os resultados obtidos em cada um dos métodos de análise descritos foram então comparados. / This paper presents a review of the methodology of microarray experiments for its installation and statistical analysis of data obtained. Then this methodology is applied in data analysis of gene expression in citrus, generated by a macroarray experiment, using linear models with fixed effects considering the inclusion or exclusion of different effects and considering adjustments of models for each gene separately and for all genes simultaneously. The macroarray experiments are similar to the microarray experiments, but use a smaller number of genes. In general, are used due to economic restrictions. Because they have been used a few arrays in the experiment analyzed in this study it was used a empirical Bayes approach that uses estimates of variance more stable and that takes into account the correlation among replicates of the gene within array. A non parametric analysis method was also used to outline the problem of the non normality for some genes. The results obtained in each of the described methods of analysis were then compared.

Neste trabalho apresenta-se uma revisão da metodologia de experimentos de microarray relativas a sua instalação e análise estatística dos dados obtidos. A seguir, aplica-se essa metodologia na análise de dados de expressão gênica em citros, gerados por um experimento de macroarray, utilizando modelos lineares de efeitos fixos considerando a inclusão ou não de diferentes efeitos e considerando ajustes de modelos para cada gene separadamente e para todos os genes simultaneamente. Os experimentos de macroarray são similares aos experimentos de microarray, porém utilizam um menor número de genes. Em geral, são utilizados devido a restrições econômicas. Devido ao fato de terem sido utilizados poucos arrays no experimento analisado neste trabalho foi utilizada uma abordagem bayesiana empírica que utiliza estimativas de variância mais estáveis e que leva em consideração a correlação entre as repetições do gene dentro do array. Também foi utilizado um método de análise não paramétrico para contornar o problema da falta de normalidade para alguns genes. Os resultados obtidos em cada um dos métodos de análise descritos foram então comparados. / This paper presents a review of the methodology of microarray experiments for its installation and statistical analysis of data obtained. Then this methodology is applied in data analysis of gene expression in citrus, generated by a macroarray experiment, using linear models with fixed effects considering the inclusion or exclusion of different effects and considering adjustments of models for each gene separately and for all genes simultaneously. The macroarray experiments are similar to the microarray experiments, but use a smaller number of genes. In general, are used due to economic restrictions. Because they have been used a few arrays in the experiment analyzed in this study it was used a empirical Bayes approach that uses estimates of variance more stable and that takes into account the correlation among replicates of the gene within array. A non parametric analysis method was also used to outline the problem of the non normality for some genes. The results obtained in each of the described methods of analysis were then compared.