The thesis deals with phenotypic, ploidy and genetic variation of two Campanula species occurring in the Krkonoše Mts., namely the endemic C. bohemica and widespread C. rotundifolia subsp. rotundifolia. For comparative purposes subspecies sudetica of the latter species was also included. The main aim was to get insight into population structure and assess the threat of interspecific hybridization to the survival of the endemic bluebell. Flow cytometry, distance-based morphometrics and molecular analysis were used to address these questions. Three distinct groups of fluorescence intensities were revealed by flow cytometry, corresponding to DNA diploids, tetraploids and pentaploids. While diploids morphologically matched the nominate subspecies of C. rotundifolia, tetraploids corresponded either to C. rotundifolia subsp. sudetica or C. bohemica. Most populations from the Krkonoše Mts. were uniform although a sympatric growth of diploids and tetraploids was encountered in 12 populations. Only two pentaploids individuals, most likely of hybrid origin, were found, which indicated that interspecific hybridization is much less common than previously assumed. The two tetraploid taxa were distinguished by molecular markers. A combination of flow cytometry and molecular analyses thus allowed reliable...

The Carlina vulgaris complex in central Europe includes several lineages defined by their ecology, morphology and distribution. This diploma thesis is focused on relationships between the taxa recognized in the Czech Republic, namely Carlina vulgaris subsp. vulgaris, C. biebersteinii subsp. biebersteinii, C. biebersteinii subsp. brevibracteata and C. biebersteinii subsp. sudetica. Molecular analysis revealed two genetically defined groups. One includes samples from relict populations in western Bohemia and from high mountains classified as C. biebersteinii subsp. biebersteinii and C. biebersteinii subsp. sudetica. The other is represented by plants classified as C. vulgaris and C. biebersteinii subsp. brevibracteata. This genetic differentiation was also confirmed by morphometric analysis. However, relationships within these two groups remain unclear. The Czech populations of Carlina biebersteinii subsp. biebersteinii as well as of C. biebersteinii subsp. sudetica are closely related to the mountain populations in the Alps and Carpathians. Their occurrence in the Czech Republic is relict and they should be in focus of nature conservation. However, the separate taxonomic position of the claimed endemic C. biebersteinii subsp. sudetica is probably unjustified.

The presented study tries to reveal the evolutionary history of polyploid complex Galium pusillum aiming on a rare czech endemic species G. sudeticum, an ideal model to study allopatric speciation in the context of Central European (post)glacial development. The species G. sudeticum grows in Krkonose Mts. and on serpentines in western Bohemia (Slavkovský les) more than 200 kilometers away. Our morphological and molecular data suggest a clearly different history of its two isolated areas. The populations in Krkonose Mts. are probable relics of mountainous species G. anisophyllon which was there on its northern border of occurence and hybridized with lowland species G. valdepilosum during postglacial vegetations shifts. This fact is well supported by intermediate position of Krkonose Mts. populations in both morphological and molecular AFLP analyses and by sharing the same chloroplast haplotype with geographically close lowland populations. On the other hand the serpentine lowland populations in western Bohemia are both morphologicaly and genetically indistinguishable from G. valdepilosum. Distinct genetic lineage of few populations belonging to G. valdepilosum (incl. one traditionaly referred as G. sudeticum) was found in western Bohemia and Bavaria, located on relic stands (calcareous and serpentine...

The aim of this thesis is to describe the changes of bird species diversity along elevation gradient on six mounts in Cameroon and nearby Bioko island. The results of the analyses confirm most of the defined hypotheses, especially they show that species diversity declines with altitude and rises with area. Cluster analysis shows the similarity of individual mountains in particular altitude zones. Despite the original hypothesis, mount Cameroon is found to be most similar to mount Kupé, although high mountain assemblages of mount Cameroon and Bioko island are very alike as well. The absolutely highest diversity was detected on mount Cameroon and mount Kupé, but above 2100 m above sea level mounts Oku and Manenguba are the most diverse. The results of this thesis contribute to a clearer notion about factors influencing species diversity; however, more thorough data (particularly from mount Nlonako) and data extension for e.g. bird abundance would enrich the study and it would enable us to perform more detailed diversity analysis, especially an analysis of competition relationship among species. Key words Species diversity, Cameroon, bird assemblages, elevational gradient

The aim of this thesis is to describe the changes of bird species diversity along elevation gradient on six mounts in Cameroon and nearby Bioko island. The results of the analyses confirm most of the defined hypotheses, especially they show that species diversity declines with altitude and rises with area. The absolutely highest diversity was detected on mount Kupé and mount Cameroon, but above 2100 m above sea level mounts Oku and Manenguba are the most diverse. Other analysis showed that the biggest changes in beta diversity of bird assemblages along altitudinal gradients are on the lowest elevational belts which are on the edges of a degraded habitats and continuous undisturbed forests. Other important changes in bird assemblages happen around altitude 1200 m, where the assemblages of lowland rainforest change to assemblages of mountain forest. Analyses of affinity of bird species to forest habitat showed that the most affined assemblages to the forest are those found on mounts Kupé and Rata. On the other hand bird assemblages on mounts Manenguba and Oku are the one of least affined to the forest biotope. Analysis of habitat generalism and specialism did not show the expected outcome and for clearer results the design of analysis needs to be improved. The results of this thesis contribute to a...

This thesis is a biosystematic study focusing on the taxonomy and variability of selected taxa of the genus Sorbus, one of the most diverse and taxonomically complicated plant groups in Europe. Classical and modern biosystematic methods - comparative study, chromosome counting, analysis of nuclear microsatellite markers, flow cytometry, and traditional multivariate and outline morphometric analyses were used to assess the morphological, karyological and genetic variability of the genus. The final synthesis of these approaches led to the description of several new taxa (species, hybrids and a subgenus) and the correction of several taxonomic misinterpretations. To reveal ongoing evolutionary processes responsible for the generation of the observed variability, the reproductive modes of 42 Sorbus taxa were examined using flow-cytometric seed and pollen screens. Apart from revealing major trends, the study estimates the frequency of rare events and provides several novel conclusions that are relevant both specifically to Sorbus/Rosaceae and to apomixis in general.