Although it has long been recognized that physiological acclimation of photosynthesis and respiration can occur in plants exposed to changing environmental conditions (e.g. light, temperature or stress), the extent of acclimation in different tissues (i.e. pre-existing and new foliage) however, has not received much attention until recently. Furthermore, few studies have investigated the extent of photosynthetic and respiratory acclimation under natural conditions, where air temperatures vary diurnally and seasonally. In this study, the effects of variations in temperature on respiratory CO2 loss and photosynthetic carbon assimilation were examined under both controlled and natural environments. The purpose of the investigations described in this thesis was to identify the effects acclimation would have on two key metabolic processes in plants exposed to temperature change, with emphasis also placed on the role of nutrition (nitrogen) and respiratory enzymatic characteristics on the potential for acclimation in two contrasting tree species, Pinus radiata and Populus deltoides. Controlled-environment studies (Chapter 2 and 3) established that rates of foliar respiration are sensitive to short-term changes in temperature (increasing exponentially with temperature) but in the longer-term (days to weeks), foliar respiration acclimates to temperature change. As a result, rates of dark respiration measured at any given temperature are higher in cold-acclimated and lower in warm-acclimated plants than would be predicted from an instantaneous response. Acclimation in new foliage (formed under the new temperature environment) was found to result in respiratory homeostasis (i.e. constant rates of foliar respiration following long-term changes in temperature, when respiration is measured at the prevailing growth temperature). Available evidence suggests that substantial adjustments in foliar respiration tend to be developmentally dependent. This may in part explain why respiratory homeostasis was only observed in new but not in pre-existing tissues. Step changes in temperature (cold and warm transfers) resulted in significant changes in photosynthetic capacity. However, in stark contrast to the findings of respiration, there was little evidence for photosynthetic acclimation to temperature change. The results obtained from field studies (Chapter 4) show that in the long-term over a full year, dark respiration rates in both tree species were insensitive to temperature but photosynthesis retained its sensitivity, increasing with increasing temperature. Respiration in both species showed a significant downregulation during spring and summer and increases in respiratory capacity were observed in autumn and winter. Thermal acclimation of respiration was associated with a change in the concentration of soluble sugars. Hence, acclimation of dark respiration under a naturally changing environment is characterized by changes in the temperature sensitivity and apparent capacity of the respiratory apparatus. The results from controlled and natural-environment studies were used to drive a leaflevel model (which accounted for dark respiratory acclimation) with the aim of forecasting the overall impact of responses of photosynthesis and respiration in the long term (Chapter 5). Modellers utilise the temperature responses of photosynthesis and respiration to parameterize carbon exchange models but often ignore acclimation and use only instantaneous responses to drive such models. The studies here have shown that this can result in erroneous estimates of carbon exchange as strong respiratory acclimation occurs over longer periods of temperature change. For example, it was found here that the failure to factor for dark respiratory acclimation resulted in the underestimation of carbon losses by foliar respiration during cooler months and an overestimation during warmer months - such discrepancies are likely to have an important impact on determinations of the carbon economy of forests and ecosystems. The overall results substantiate the conclusion that understanding the effect of variations in temperature on rates of carbon loss by plant respiration is a prerequisite for predicting estimates of atmospheric CO2 release in a changing global environment. It has been shown here that within a moderate range of temperatures, rate of carbon uptake by photosynthesis exceeds the rate of carbon loss by plant respiration in response to warming as a result of strong respiratory acclimation to temperature change. This has strong implications for models which fail to account for acclimation of respiration. At present, respiration is assumed to increase with increasing temperatures. This erroneous assumption supports conclusions linking warming to the reinforcement of the greenhouse effect.

Experiments were carried out to study the effect of workmanship and environmental conditions on bond strength for concrete patch repairs. Four repair materials, sand/cement mortar, acrylic modified cementitious mortar, SBR modified cementitious mortar, and flowing concrete, were tested with mainly three test methods (core pull-off test, patch compressive test, and patch flexural test). At the beginning of this project, slant shear tests were also carried out. In the study of the effect of workmanship, the following parameters were included: surface roughness, surface cleanliness, surface soundness, moisture condition, application method, bond coat mistiming, repair material mistiming, and curing methods. In the study of the effect of environmental conditions, four parameters were considered: high temperature curing followed by drying shrinkage, high temperature curing followed by thermal cycling, low temperature curing, and low temperature curing followed by freeze/thaw cycling. A rougher surface produces a higher bond strength, but the increase depends on individual repair material. Sand/cement mortar favours a rough surface, but polymer modified mortars are not very sensitive to surface roughness. Environmental conditions affect the bond strength development, but the effect varies with each repair material. Test results suggest that low temperature curing should be avoided for polymer modified cementitious mortars. In addition to the experimental study, theoretical analyses were carried out to evaluate the available bond test methods. The evaluation was concentrated on answering the following questions: (1) What kind of factors will influence conductinga bond test? (2) What are the response of each factor involved to a specific test method? (3) What kind of influences are crucial in ensuring the full development of the bond strength? (4) Which factors are important to achieve a durable repair? and (5) What kind of a test can be used to monitor the quality of these crucial factors? In total, about 800 tests were conducted (500 core pull-off tests, 90 patch compressive tests, 100 patch flexural tests, and 80 slant shear tests).

Although it has long been recognized that physiological acclimation of photosynthesis and respiration can occur in plants exposed to changing environmental conditions (e.g. light, temperature or stress), the extent of acclimation in different tissues (i.e. pre-existing and new foliage) however, has not received much attention until recently. Furthermore, few studies have investigated the extent of photosynthetic and respiratory acclimation under natural conditions, where air temperatures vary diurnally and seasonally. In this study, the effects of variations in temperature on respiratory CO2 loss and photosynthetic carbon assimilation were examined under both controlled and natural environments. The purpose of the investigations described in this thesis was to identify the effects acclimation would have on two key metabolic processes in plants exposed to temperature change, with emphasis also placed on the role of nutrition (nitrogen) and respiratory enzymatic characteristics on the potential for acclimation in two contrasting tree species, Pinus radiata and Populus deltoides. Controlled-environment studies (Chapter 2 and 3) established that rates of foliar respiration are sensitive to short-term changes in temperature (increasing exponentially with temperature) but in the longer-term (days to weeks), foliar respiration acclimates to temperature change. As a result, rates of dark respiration measured at any given temperature are higher in cold-acclimated and lower in warm-acclimated plants than would be predicted from an instantaneous response. Acclimation in new foliage (formed under the new temperature environment) was found to result in respiratory homeostasis (i.e. constant rates of foliar respiration following long-term changes in temperature, when respiration is measured at the prevailing growth temperature). Available evidence suggests that substantial adjustments in foliar respiration tend to be developmentally dependent. This may in part explain why respiratory homeostasis was only observed in new but not in pre-existing tissues. Step changes in temperature (cold and warm transfers) resulted in significant changes in photosynthetic capacity. However, in stark contrast to the findings of respiration, there was little evidence for photosynthetic acclimation to temperature change. The results obtained from field studies (Chapter 4) show that in the long-term over a full year, dark respiration rates in both tree species were insensitive to temperature but photosynthesis retained its sensitivity, increasing with increasing temperature. Respiration in both species showed a significant downregulation during spring and summer and increases in respiratory capacity were observed in autumn and winter. Thermal acclimation of respiration was associated with a change in the concentration of soluble sugars. Hence, acclimation of dark respiration under a naturally changing environment is characterized by changes in the temperature sensitivity and apparent capacity of the respiratory apparatus. The results from controlled and natural-environment studies were used to drive a leaflevel model (which accounted for dark respiratory acclimation) with the aim of forecasting the overall impact of responses of photosynthesis and respiration in the long term (Chapter 5). Modellers utilise the temperature responses of photosynthesis and respiration to parameterize carbon exchange models but often ignore acclimation and use only instantaneous responses to drive such models. The studies here have shown that this can result in erroneous estimates of carbon exchange as strong respiratory acclimation occurs over longer periods of temperature change. For example, it was found here that the failure to factor for dark respiratory acclimation resulted in the underestimation of carbon losses by foliar respiration during cooler months and an overestimation during warmer months - such discrepancies are likely to have an important impact on determinations of the carbon economy of forests and ecosystems. The overall results substantiate the conclusion that understanding the effect of variations in temperature on rates of carbon loss by plant respiration is a prerequisite for predicting estimates of atmospheric CO2 release in a changing global environment. It has been shown here that within a moderate range of temperatures, rate of carbon uptake by photosynthesis exceeds the rate of carbon loss by plant respiration in response to warming as a result of strong respiratory acclimation to temperature change. This has strong implications for models which fail to account for acclimation of respiration. At present, respiration is assumed to increase with increasing temperatures. This erroneous assumption supports conclusions linking warming to the reinforcement of the greenhouse effect.

In this study, the corallite shapes of three species of the scleractinian genus Undaria from the Yague group, Dominican Republic, were examined through a period of time stretching from 6.4 mya to 3.4 mya, a total of 3.0 ma. Corallite shape was measured using 3 dimensional landmarks and manipulated using the well established procedures of geometric morphometrics. Differences in shape and size through time were examined using a variety of tools, including canonical variates analysis, principal components analysis, least squares regression, partial least squares regression, and a variety of evolutionary model fits. Evolutionary model fits were used to test three models against the shape and size variables: general random walk, which models a directional change through time; unbiased random walk, which models random change through time; and stasis, which models stability through time. Stasis is the most common parameter through time, supported in 9 of 15 (60%) of cases, while the unbiased random walk was supported 6 of 15 times. While there was a significant change in one species associated with environmental variables, those variables were also correlated with time and no causal relationship can be reached.

In this study, it is hypothesized that the mesoscale environment can indirectly control the cloud-to-ground (CG) lightning polarity of severe storms by directly affecting their structural, dynamical, and microphysical properties, which in turn directly control cloud electrification and CG flash polarity. A more specific hypothesis, which has been supported by past observational and laboratory charging studies, suggests that broad, strong updrafts and associated large liquid water contents in severe storms lead to enhanced positive charging of graupel and hail via the noninductive charging mechanism, the generation of an inverted charge structure, and increased positive CG lightning production. The corollary is that environmental conditions favoring these kinematic and microphysical characteristics should support severe storms generating an anomalously high (> 25%) percentage of positive CG lightning (i.e., positive storms), while environmental conditions relatively less favorable should sustain storms characterized by a typical (≤ 25%) percentage of positive CG lightning (i.e., negative storms). Forty-eight inflow proximity soundings were analyzed to characterize the environments of nine distinct mesoscale regions of severe storms (four positive and five negative) on six days during May – June 2002 over the central United States. This analysis clearly demonstrated significant and systematic differences in the mesoscale environments of positive and negative storms, which were consistent with the stated hypothesis. When compared to negative storms, positive storms occurred in environments associated with a drier low to midtroposphere, higher cloud base height, smaller warm cloud depth, stronger conditional instability, larger 0-3 km AGL wind shear, stronger 0-2 km AGL storm-relative wind speed, and larger buoyancy in the mixed-phase zone, at a statistically significant level. Differences in the warm cloud depth of positive and negative storms were by far the most dramatic, suggesting an important role for this parameter in controlling CG lightning polarity. Subjective visual inspection of radar imagery revealed no strong relationship between convective mode and CG lightning polarity, and also illustrated that positive and negative severe storms can be equally intense.

In this study, it is hypothesized that the mesoscale environment can indirectly control the cloud-to-ground (CG) lightning polarity of severe storms by directly affecting their structural, dynamical, and microphysical properties, which in turn directly control cloud electrification and CG flash polarity. A more specific hypothesis, which has been supported by past observational and laboratory charging studies, suggests that broad, strong updrafts and associated large liquid water contents in severe storms lead to enhanced positive charging of graupel and hail via the noninductive charging mechanism, the generation of an inverted charge structure, and increased positive CG lightning production. The corollary is that environmental conditions favoring these kinematic and microphysical characteristics should support severe storms generating an anomalously high (> 25%) percentage of positive CG lightning (i.e., positive storms), while environmental conditions relatively less favorable should sustain storms characterized by a typical (≤ 25%) percentage of positive CG lightning (i.e., negative storms). Forty-eight inflow proximity soundings were analyzed to characterize the environments of nine distinct mesoscale regions of severe storms (four positive and five negative) on six days during May - June 2002 over the central United States. This analysis clearly demonstrated significant and systematic differences in the mesoscale environments of positive and negative storms, which were consistent with the stated hypothesis. When compared to negative storms, positive storms occurred in environments associated with a drier low to midtroposphere, higher cloud base height, smaller warm cloud depth, stronger conditional instability, larger 0-3 km AGL wind shear, stronger 0-2 km AGL storm-relative wind speed, and larger buoyancy in the mixed-phase zone, at a statistically significant level. Differences in the warm cloud depth of positive and negative storms were by far the most dramatic, suggesting an important role for this parameter in controlling CG lightning polarity. Subjective visual inspection of radar imagery revealed no strong relationship between convective mode and CG lightning polarity, and also illustrated that positive and negative severe storms can be equally intense.

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Landscape characteristics affect vineyard microclimates and influence the quality of grapes produced. There is currently a lack of research examining the microclimatological conditions of vineyards and how they affect grape quality. This study utilizes qualitative methods to explore the relationship between vineyard microclimates and grape quality in Madeira, Portugal. Semi-structured interviews have been conducted with local participants involved in the production of wine grapes and are used to reveal the factors affecting grape quality. Interviews accompany a physical inventory of landscape characteristics in the vineyards, and are guided by where interviewees observe changes in grape quality. The findings suggest there is a strong relationship between microclimates and grape quality. These results are utilized to make recommendations for methods of site selection and the microclimatic design of vineyards.

During the last decade, Mexico City's air quality has deteriorated dramatically. Air pollution management has become a major issue, and a number of policies and campaigns aimed at reducing the volume of harmful emissions released into the atmosphere by vehicles and large-scale industries, have been implemented. / Lighting, office equipment, cooking, refrigeration, space heating, space cooling, ventilation, and water heating are the ultimate commercial uses of energy. The goal of this study is to determine if there is a potential market for solar water heaters that could provide hot water for a number of activities in the city, reducing the amount of fossil fuels burned for this purpose, thus contributing to decrease the amount of air pollutants to the atmosphere. / The results of this research show how a number of industries, public services and commercial activities need to be provided with both water and energy in large quantities, and are therefore potential users of solar thermal technologies.