<p> Mining is widespread and an economically important industry. Unfortunately, acid mine drainage (AMD) can pollute ecosystems with a cocktail of contaminants too complex for accurately forecasting its health consequences. However, through quantification of fundamental toxic events, the effects of complex mixtures can be observed. This project explored two potentially insightful and convenient endpoints. First, oxygen consumption (MO₂), a well-established and sensitive indicator of respiratory impairment was utilized. Second, protein carbonyl content (PCC), an experimental ecological biomarker widely lauded in biomedical circles as a highly conserved indicator of health status was assessed for its utility in a metal tolerant aquatic macroinvertebrate, <i> Arctopsyche grandis. A. grandis</i> were exposed to eight environmentally relevant target concentrations (in duplicate) of AMD for eight days at a temperature controlled greenhouse containing artificial flow-through streams. As expected, MO₂ was inversely related to treatment concentration (R²=0.35, p=0.015). Protein carbonyl content, however, diverged from predictions. Protein carbonyl content analysis detected significantly more oxidative protein injury in control treatments than in metal-rich AMD treatments (p&lt;0.001). Moreover, there was not a significant difference in PCC between different AMD concentrations. Protein carbonyl content&rsquo;s departure from anticipated results likely is the consequence of dynamic interactions between direct and indirect effects at the chemical, biochemical, physiologic and behavioral levels. The results of this project illustrate flaws of utilizing a single biochemical marker to observe effects of a toxic mixture. Rather, a broad suite of biomarkers should be assayed to determine sublethal toxicity. These results also illustrates how multiple stressors can yield unanticipated outcomes.</p>

<p> Anthropology has a long history collaborating with artists to understand their artwork. However, little research exists in the discipline that focuses on artists as a group, their creative process, and what may influence that process. In particular, how artists use nature and place has not been studied; instead, anthropology has generally considered nature and place as merely a backdrop for culture rather than for its impact on cultural expression. Identification of diverse aspects of the interdependence of ecological and social systems can inform our understanding of how people address issues of environmental concern. Managers, scientists, creative people, and others working at the nexus of disciplines, management needs, and ecological and social systems can facilitate this understanding through knowledge sharing. In my research I examined how two groups of visual artists process their interaction with the environment through what I term &ldquo;experiencing with&rdquo; nature and how this may influence them as artists. </p><p> I employed phenomenological inquiry methods and interdisciplinary analysis to investigate the ways in which artists develop a sense of experiencing with nature and a sense of place. I developed an experiencing formula framework representing relationships between variables involved in the act of experiencing in order to analyze artists&rsquo; narratives and actions as a way to examine their perceptions of their experiences with nature. The analysis made evident six primary categories of findings: artists&rsquo; sense of experiencing with nature, their purpose of experiencing, their process of experiencing, their conceptual definitions of nature, their access to nature, and how they experienced nature through the artist residency programs. I propose the experiencing formula framework may be suitable for describing human-environment relationships beyond the boundaries of artists and nature. </p><p> The artists&rsquo; experiences were individual and influenced them to varying degrees. They experienced nature with purpose and encountered both tension and inspiration while gathering resources for their work. They were not so concerned with defining nature as seeking to tell their story of place through their sense of experiencing to communicate their experiences with nature through their works. Experiencing with nature provided them with a language for expressing themselves. Nature was a place for journey and exploration for the artists.</p>

<p> Seagrass ecosystems are some of the most productive in the world and provide a variety of ecosystem services but are facing global decline chiefly due to anthropogenic disturbance. Mechanical disturbances to seagrass meadows from anchoring, propeller scars, and mooring scars result in losses or damage to both shoots and the underlying rhizome. I conducted a literature synthesis on the extent of, recovery from, and ecological impacts of these mechanical disturbances to seagrass meadows. The literature suggests that anchoring damage tends to be worst in deeper water where larger vessels anchor and can cause large (>100m²) loss per anchoring and recovery may take decades to over a century. Propeller damage is of largest concern in shallow (&lt;2m) areas experiencing heavy boat traffic because propeller damage can only occur where the propeller can come close to the seagrass. Mooring damage is highly variable depending on the type of mooring used (&lt;10m² to >1000m² scoured per mooring). Seagrass patches experiencing these mechanical disturbances have, in some studies, been found to have lower seagrass percent cover and shoot density than reference areas. This indicates scars can have &lsquo;halos&rsquo; of impacted seagrass meadow. Some seagrass systems cannot recover within a century (e.g. <i>Posidonia oceanica</i> meadows at the extent of their depth tolerance) while others recover annually from some disturbances (e.g., anchor scars &lt;1m²). Systems face altered species composition when scars are preferentially recolonized by certain species and patterns of recovery are affected by altered biogeochemical conditions following disturbances. Additionally, mooring, anchoring, and propeller scarring frequently alter meadow density, cover, patch size, patch shape, patch isolation, edge area, and ratios of edge to interior meadow leading to changes in faunal community structure. Correlations between these disturbances and faunal abundances, densities, and richness in seagrass ecosystems are complex, vary temporally (sometimes on the scale of days), and may result in species showing positive, negative, or no responses to a wide range of disturbance regimes. </p><p> To explore the connections between mooring scarring, the surrounding seagrass meadow condition and epifaunal community, in the second part of this thesis I measured 30 mooring scars to determine average scar size. To explore any potential &lsquo;halo&rsquo; effect around mooring scars for seagrass or epifauna and to seek any difference in epifaunal community between mooring and reference sites I also sampled paired sites at eight locations in Nantucket Harbor, Massachusetts three times each in the summer of 2015. Each location consisted of a meadow site actively experiencing mooring scarring and a reference site without moorings. My conservative sampling methods of the 30 sampled mooring scars found scars to average 21.1m². Across my paired sites, seagrass was found to have lower cover and lower canopy height in mooring versus reference sites. Seagrass cover and canopy height were lower in the first few meters (typically 2-3m) surrounding each scar in comparison to paired reference quadrats indicating a &lsquo;halo&rsquo; effect of each scar. I did not detect a difference in epifaunal community composition or density per blade between mooring and reference sites; however, the relatively constant per blade concentration of epifauna combined with the differences in seagrass biomass between the mooring versus reference sites indicate an overall increase in the total amount of epifauna in areas with less disturbance. Epifaunal community composition was different and between locations and sampling dates indicating these factors are more important than proximity to mooring scarring in determining epifaunal abundance and richness. When considering scar area and the &lsquo;halo&rsquo; of each scar I estimate that at least 32ha (2%) of Nantucket Harbor was impacted by mooring scarring. Given that the estimates of seagrass do not include areas previously denuded of this plant and that my measurements were conservative, likely a larger portion of this harbor&rsquo;s potential seagrass habitat is impacted. Combined with the findings of loss due to these direct boat-related physical disturbances of seagrass meadows worldwide across regions, this halo effect is likely to also be found for moorings globally. I encourage management of the issue by employing and fine-tuning mooring methods (such as deploying moorings with anchor connections that do not drag on the sea floor) to minimize these unintentional but strong effects of mooring on the recipient habitat.</p><p>

<p> The current debate over whether conventional or organic farming is superior has produced an abundance of literature, however, only a small portion is devoted to mineral composition of foods ready for consumption. The purpose of this study was to compare mineral content in conventional versus organic foods. Specifically, this study tested almonds because they are a convenient and popular product. Inductively Coupled Plasma - Atomic Emission Spectrometry was used to analyze triplicate samples of conventional and organic almonds for quantities of the following essential minerals: calcium, iron, magnesium, potassium, and zinc. </p><p> Results of the analyses showed no significant differences in conventional versus organic almonds for calcium (<i>p</i> = .359), iron (<i> p</i> = .368), magnesium (<i>p</i> = .921), potassium (<i> p</i> =.129), or zinc (<i>p</i> = .875). Although this data suggests little difference in mineral content between conventional versus organic almonds, the small sample size (<i>n</i> = 6) provides unsubstantial evidence that a difference does not exist in larger samples.</p><p>

<p> The anthropogenic influences on our climate system has caused an unprecedented increase of carbon emissions amongst other greenhouse gases into our atmosphere resulting in energy imbalances and observed positive feedbacks. The American Southwest is witnessing major urban transformation as its cities are becoming centers for urban growth. Without adopting climatic design, this continued sprawl will greatly compromise the natural environment and, human health and wellbeing. Intensive urbanization has the greatest risk for increasing the carbon footprint, environmental pollution, urban heat island phenomenon, and infrastructure overextension. Increasing ground surface temperatures; the outdoor environment is observing record breaking temperature highs, while the artificial indoor set points stay the same to counter that effect.</p><p> Buildings placed in areas of excessive heat and glaring sun are in need of a comprehensive approach to address their environments. With the advancements in technology, trends have shown that architects and designers are shifting towards engineering solutions to artificially control their environments to a static state. While spaces steer away from passive designs, their operation demand high energy consumption and their occupants are deprived of a sense of nature.</p><p> Climatic design is a key ingredient to the success of an optimized environment, or to its failure. Human Health is one component that is most challenged and could be most responsive to that environment and its built parameters. The important role that these components play are greater when immersed in hot-arid regions where resources are scarce, and the built environment finds itself in a hostile environment where it struggles to survive, relying heavily on the consumption of fossil fuels.</p><p> The goal of this research is to develop a methodology in which the human physiological responses are centered in the role of design. Through examination of this phenomenon and recommendations based on the findings, developers will be informed of the importance of human-centered climatic design. Data driven to optimize both human health and the environmental footprint. Utilization of this method will not only decrease the contribution of the built environment but will also decrease the chronic exposure of humans to these high contrasts and pave the way to cautious practices optimizing health of the environment. The long-term goal is to develop indices based on human-centered climatic design to inform our design decisions and represent a flagship to a sustainable way of living. The objective of this research is to validate the dialogue between climatic design and human physiological comfort. The hypothesis is that a specific list of elements in man-made built environment trigger responses in both the atmosphere and humans. The rationale behind this methodology is based on the role of architecture as a primary instrument that could cause imbalances in both the climate and the human body.</p><p> To accomplish our objective and test our central hypothesis the authors outlined three broad environments; (climate, architecture, and human) to further investigate the relationship centered around the human body and its multisensory experience. Results of this study will allow the identification of an articulate common solution focused on transitional spaces.</p><p> The contribution of knowledge in this research is outlined in three sequential publications. The first adopts a comprehensive approach that redefines space and the elements in three environments that have an impact on it. The second leads with the previous developed relationship (from paper 1) to develop a human physiological comfort index applicable within a broader architecture foundation, and the third utilizes this index to demonstrate techniques developed in this research to measure climatic and human attributes objectively, and non-obtrusively. At the final stage these disciplines will be overlaid to create architecture unity in a sequence of variables which this document will follow. The conclusion outlines a series of concepts that represent human-centered climatic design applications.</p><p> This dissertation graphically articulates the interactive network of dialogue between humans and space to serve as a balanced approach to architectural design. The genesis of this investigation is supported by the development of a protocol that utilizes interdisciplinary research methodology that couples theoretical and empirical based discoveries to inform the fundamentals of the built environment, standards, and operations optimizing occupant health and wellbeing in extreme environments. By combining spatial design with health parameters, architects will be able to make decisions that will reduce energy consumption, decrease the impact on climate change; ultimately protect the natural world and optimally support future life. The actions taken in response to this study will not only decrease the direct impact of large temperature differences that trigger human stress responses, but will also reduce the emission of anthropogenic greenhouse gases.</p><p>

<p> Mountaintop removal/valley fill (MTR/VF) coal mining in the Central Appalachian region has created the most extreme anthropogenic landscape on Earth by lowering ridges and infilling headwater stream valleys. No studies have attempted to detail erosional processes active on MTR/VR landforms. A combination of field work and LiDAR data observations documents erosional features on MTR/VF landscapes. Landscape evolution modeling explores future possibilities in valley-filled catchments. LiDAR data also allows for the quantification of valley-filled catchment alteration with statistically significant differences in both drainage density and depression storage capacity between &ldquo;less disturbed&rdquo; and valley-filled catchments. Field and LiDAR data observations show that the drainage systems associated with the periphery of the MTR/VF landscape are particularly vulnerable to gully erosion. This study also provides evidence of landslides occurring within fully reclaimed valley fills. Landscape evolution modeling reproduces gully erosion mechanisms documents in the field. Modeled erosion rates based solely on bedload averaged 10.9 mm/kyr^-1. Modeled erosion rates are higher on valley fills with constructed drains in the center of the valley fill relative to those with drains along the sides.</p><p>

abstract: Hydrology and biogeochemistry are coupled in all systems. However, human decision-making regarding hydrology and biogeochemistry are often separate, even though decisions about hydrologic systems may have substantial impacts on biogeochemical patterns and processes. The overarching question of this dissertation was: How does hydrologic engineering interact with the effects of nutrient loading and climate to drive watershed nutrient yields? I conducted research in two study systems with contrasting spatial and temporal scales. Using a combination of data-mining and modeling approaches, I reconstructed nitrogen and phosphorus budgets for the northeastern US over the 20th century, including anthropogenic nutrient inputs and riverine fluxes, for ~200 watersheds at 5 year time intervals. Infrastructure systems, such as sewers, wastewater treatment plants, and reservoirs, strongly affected the spatial and temporal patterns of nutrient fluxes from northeastern watersheds. At a smaller scale, I investigated the effects of urban stormwater drainage infrastructure on water and nutrient delivery from urban watersheds in Phoenix, AZ. Using a combination of field monitoring and statistical modeling, I tested hypotheses about the importance of hydrologic and biogeochemical control of nutrient delivery. My research suggests that hydrology is the major driver of differences in nutrient fluxes from urban watersheds at the event scale, and that consideration of altered hydrologic networks is critical for understanding anthropogenic impacts on biogeochemical cycles. Overall, I found that human activities affect nutrient transport via multiple pathways. Anthropogenic nutrient additions increase the supply of nutrients available for transport, whereas hydrologic infrastructure controls the delivery of nutrients from watersheds. Incorporating the effects of hydrologic infrastructure is critical for understanding anthropogenic effects on biogeochemical fluxes across spatial and temporal scales. / Dissertation/Thesis / Ph.D. Biology 2013

<p> Severe drought plays a critical role in altering the magnitude and interannual variability of the net terrestrial carbon sink. Drought events immediately decrease net primary production (NPP), and drought length and magnitude tend to enhance this negative impact. However, satellite and in-situ measurements have also indicated that ecosystem recovery from extreme drought can extend several years beyond the return to normal climate conditions. If an ecosystem&rsquo;s drought recovery time exceeds the time interval between successive droughts, these legacy effects may reinforce the impact of future drought. Since the frequency and severity of extreme climate events are expected to increase with climate change, both the immediate and prolonged impact of drought may contribute to amplified climate warming by decreasing the strength of the land carbon sink. However, it is unknown whether terrestrial biosphere models capture the impact of drought legacy effects on carbon stocks and cycling. Using a suite of twelve land surface models from the Multi-scale Synthesis and Terrestrial Model Intercomparison Project (MsTMIP), we assessed model ability to simulate drought legacy effects by analyzing the modeled NPP response to drought events across forested regions of the US and Europe. We found that modeled drought legacy effects last about one year (2% reduction in NPP), with complete NPP recovery in the second post-drought year. Since observations suggest that legacy effects extend up to four years post-drought, with a 9% growth reduction in the first post-drought year, models appear to underestimate both the timescales and magnitude of drought legacy effects. We further explored vegetation sensitivity to climate anomalies through global, time-lagged correlation analysis of NPP and climatic water deficit. Regional differences in the lag time between climate anomaly and NPP response are prevalent, but low sensitivities (correlations) characterize the entire region. Significant correlations coincided with characteristic lag times of 0 to 6 months, indicating relatively immediate NPP response to moisture anomalies. Model ability to accurately simulate vegetation&rsquo;s response to drought and sensitivity to climate anomalies is necessary in order to produce reliable forecasts of land carbon sink strength and, consequently, to predict the rate at which climate change will progress in the future. Thus, the discrepancies between observed and simulated vegetation recovery from drought points to a potential critical model deficiency.</p><p>

<p> Crabs in the family Majoidae camouflage by decorating their exoskeletons with organisms and debris from their environment. This form of camouflage, involving both the act of decorating and carrying of these decorations, is thought to be energetically costly, and may present a trade-off under stressful environmental conditions. The energetic cost of decoration behavior has been evinced by reduced organic content due to elevated metabolism. In the context of previous research demonstrating that many marine calcifiers experience metabolic costs under experimental ocean acidification conditions, we hypothesized that decorator crabs exposed to reduced pH will have insufficient energy to support regulatory processes along with decoration behavior. Thus, we predicted that energy will be allocated towards growth and calcification at the expense of decoration behavior. Dwarf teardrop crabs, <i>Pelia tumida</i>, were exposed to ambient (pH = 8.0, pCO₂ = 613 &micro;atm) and reduced (pH = 7.75, pCO₂ = 894 &micro;atm) pH conditions for five weeks. Half of the animals in each treatment were given two sponge species, <i> Halichondria panacea</i> and <i>Haliclona permollis</i>, to decorate with, whereas the remaining animals were not allowed to decorate. At the end of the experiment, all animals were analyzed for exoskeleton mineral content (Ca and Mg) using EDX and ICP-MS, organic content (a proxy for metabolism) using TGA, and decoration behavior by quantifying sponge mass and percent cover. Overall, decorator crabs showed no signs of energy limitation under reduced pH conditions. Neither growth, exoskeleton mineral content, nor organic content of crabs differed among pH or decoration treatments. In addition, both sponge mass and percent cover remained the same across pH treatments, indicating no effect of reduced pH on decoration behavior, and thus the ability to camouflage. The maintenance of physiological processes without metabolic costs in <i>P. tumida</i> exposed to reduced pH radiates from the emerging trends on the susceptibility of crustaceans to changes in ocean chemistry associated with ocean acidification.</p><p>

<p> <i>Polylepis</i> forests are a unique high-elevation forests dominated by trees of the <i>Polylepis</i> (Rosaceae) genus found only in South America. These forests are distinctive not only for growing at some of the highest elevations in the world, but because they are distributed as isolated forest islands inside a landscape otherwise dominated by grasses. This distribution is due in part to human disturbance on the landscape and there is interest in restoring cover of <i>Polylepis</i>. Understanding of regeneration dynamics and seedling ecology of <i>Polylepis</i> can help in the management of forests and the restoration of this forest cover, but there are important gaps in knowledge regarding theses aspects in most species. For the expansion of forest cover into current grassland areas, it is important to understand the ability of seedlings to colonize, survive and grow in these areas and identify important biotic and abiotic barriers to this. In this dissertation, I contribute to our understanding of these ecological aspects for two species of <i>Polylepis</i> (<i>P. sericea </i> and <i>P. weberbaueri</i>) using a series observational studies and manipulative experiments in forests found between 4000&ndash;4500 masl in Huascaran National Park (Ancash, Peru). </p><p> In Chapter 1, I surveyed patterns of seedling dispersion across forest-grassland boundaries along the elevational gradient and identified significant associations of seedling densities to environmental covariates at local and landscape scales. I found that seedling densities of both species decrease drastically at the edge of the forest canopy, and few seedlings are found in the grassland even within meters of the forest, suggesting significant barriers to seedling establishment in the grassland. Additionally, seedling densities of each species showed patterns with elevation and solar irradiation that mirrored those of adult distributions on the landscape and were different from each other, suggesting that each species has unique environmental tolerances that manifest in the seedling stage and are important to consider in their management and restoration. </p><p> In Chapter 2, I used a series of livestock exclusion experiments to examine the influence of domestic livestock on natural regeneration inside and outside the forest and test the hypothesis that livestock are a significant barrier to seedling establishment at forest-grassland edges. After one year of livestock exclusion, I found only partial support for this hypothesis. In <i>P. sericea</i> forest stands, seedling densities increased, and recruitment and seedling growth was higher in fenced grassland areas. However, in <i> P. weberbaueri</i> stands there was little change in juvenile densities and no differences in seedling performance, except that recruitment was lower in fenced forests areas compared to unfenced forest. This showed that the influences of livestock on <i>Polylepis</i> seedling may be different depending on the habitat and species of <i>Polylepis</i>. Additionally, we found that seedling survival was surprisingly high, and similar between forest and grassland habitat. </p><p> In Chapter 3, I used sowing and &ldquo;wildling&rdquo; transplant experiments within the previous exclosure experiments to tease apart early ontogenetic filters to seedling establishment in the grassland compared to the forest. I found that the seedling emergence stage is a strong bottleneck for seedling establishment in the grassland. Furthermore, I found that <i>P. weberbueri </i> seedling density inside the forest appears to be seed limited, and can be increased by sowing. Transplanted seedlings survival was lower than found previously for natural seedlings, but was also similar between both habitats. However, it appears that livestock depresses <i>P. sericea </i> transplant survival inside the forest. Only <i>P. weberbueri </i> seedlings showed differences in growth between habitats, declining in height in the grassland, but there was only evidence that livestock suppressed growth. I concluded that seedling transplant will be the most efficient way to increase seedlings in the grassland for restoration. </p><p> Together, the results of this dissertation suggest that both unassisted and assisted expansion of natural <i>Polylepis</i> forests faces significant barriers, in particular low seedling emergence in grassland is a strong bottleneck to establishment in this habitat. It remains unclear whether livestock are generally a barrier to forest expansion, as their effects appear to be specific to each species of <i>Polylepis</i>. Here, <i> P. sericea</i> appears to be more sensitive in both forest and grassland to livestock activity than <i>P. weberbaueri</i>, but future work should address longer-term effects of livestock exclusion to confirm the population trajectories observed after one year. Finally, wilding transplants of these species can be used in restoration to overcome the barrier emergence poses to <i>Polylepis</i> establishment in grassland, although with further research, seeding schemes may prove a viable strategy</p><p>