1 |
Role of Epithelium-specific ETS Transcription Factor-1 in Airway Epithelial RegenerationOliver, Jordan 26 March 2012 (has links)
Human epithelium-specific ETS transcription factor-1 (ESE-1), which is also known as E74-like factor-3 (Elf3) in mice, is strongly expressed in lung during fetal development and in certain lung cancers. The primary goal of the work presented in this thesis was to investigate whether ESE-1 is involved in regeneration of the injured lung epithelium by administering naphthalene to both wild-type (Elf3 +/+) and Elf3-deficient (Elf3 -/-) mice. However, optimal conditions for proper utilization of the naphthalene-induced lung injury model must first be established. Therefore, dose-response studies were initially conducted by administering three different doses of naphthalene to both male and female mice, as described in chapter 2. Although it is shown that the extent of naphthalene-induced Clara cell injury is dose-dependent in both male and female mice, female mice are more sensitive to naphthalene-induced injury than male mice independent of the dose. Furthermore, it is also demonstrated that these gender-dependent differences in naphthalene injury can subsequently influence downstream lung repair kinetics. In light of these findings, lung regeneration was examined in both sexes of both Elf3 +/+ and Elf3 -/- mice. As reported in chapter 3, the kinetics of bronchiolar epithelial cell proliferation and differentiation is delayed considerably in Elf3 -/- mice following naphthalene injury. Moreover, expression of transforming growth factor-beta type II receptor, which is a well-known transcriptional target gene of ESE-1 and is involved in the induction of epithelial cell differentiation, is significantly lower in the bronchiolar airway epithelium of Elf3 -/- mice as compared to Elf3 +/+ mice under steady-state conditions and during repair of naphthalene-induced damage. Collectively, these findings occur to a similar extent in both sexes of both Elf3 +/+ and Elf3 -/- mice, and suggest that ESE-1 plays an important role in regulating the kinetics of airway epithelial regeneration after acute lung injury.
|
2 |
Role of Epithelium-specific ETS Transcription Factor-1 in Airway Epithelial RegenerationOliver, Jordan 26 March 2012 (has links)
Human epithelium-specific ETS transcription factor-1 (ESE-1), which is also known as E74-like factor-3 (Elf3) in mice, is strongly expressed in lung during fetal development and in certain lung cancers. The primary goal of the work presented in this thesis was to investigate whether ESE-1 is involved in regeneration of the injured lung epithelium by administering naphthalene to both wild-type (Elf3 +/+) and Elf3-deficient (Elf3 -/-) mice. However, optimal conditions for proper utilization of the naphthalene-induced lung injury model must first be established. Therefore, dose-response studies were initially conducted by administering three different doses of naphthalene to both male and female mice, as described in chapter 2. Although it is shown that the extent of naphthalene-induced Clara cell injury is dose-dependent in both male and female mice, female mice are more sensitive to naphthalene-induced injury than male mice independent of the dose. Furthermore, it is also demonstrated that these gender-dependent differences in naphthalene injury can subsequently influence downstream lung repair kinetics. In light of these findings, lung regeneration was examined in both sexes of both Elf3 +/+ and Elf3 -/- mice. As reported in chapter 3, the kinetics of bronchiolar epithelial cell proliferation and differentiation is delayed considerably in Elf3 -/- mice following naphthalene injury. Moreover, expression of transforming growth factor-beta type II receptor, which is a well-known transcriptional target gene of ESE-1 and is involved in the induction of epithelial cell differentiation, is significantly lower in the bronchiolar airway epithelium of Elf3 -/- mice as compared to Elf3 +/+ mice under steady-state conditions and during repair of naphthalene-induced damage. Collectively, these findings occur to a similar extent in both sexes of both Elf3 +/+ and Elf3 -/- mice, and suggest that ESE-1 plays an important role in regulating the kinetics of airway epithelial regeneration after acute lung injury.
|
3 |
Fonction de CFTR dans les processus de réparation de l’épithélium des voies aériennes et développement de nouvelles stratégies thérapeutiques en fibrose kystiqueTrinh, Nguyen Thu Ngan 04 1900 (has links)
La pathologie de la fibrose kystique (FK) est causée par des mutations dans le gène codant pour le canal CFTR. La mutation la plus commune est la délétion du résidu Phe508 (∆F508), qui entraîne un mauvais repliement et la dégradation de la protéine mutée. Ainsi, l’absence du CFTR cause un dysfonctionnement du transport ionique et liquidien qui altère le phénomène de clairance mucociliaire. Il en résulte une accumulation de mucus visqueux obstruant les voies aériennes favorisant une colonisation bactérienne, spécialement par P. aeruginosa, et une inflammation chronique. Ces phénomènes entraînent des lésions épithéliales et un remodelage des voies aériennes. Selon nos analyses ultrastructurales de poumons issus de patients FK au moment de la transplantation, certaines zones de l’épithélium FK montrait des signes de d’initiation des processus de réparation. Malgré cela, un dommage épithélial progressif est observé chez les patients FK et il apparaît évident que les processus de réparation sont insuffisants pour permettre le rétablissement de l’intégrité épithéliale.
Le principal objectif de mon étude était d’étudier le rôle du CFTR dans les mécanismes de réparation de l’épithélium FK et de déterminer l’impact de la correction du CFTR sur la réparation épithéliale et ce, en condition aseptique et en présence d’infection. Mes travaux montrent que l’épithélium des voies aériennes FK présente un défaut de réparation, associé, du moins en partie, à l’absence d’un CFTR fonctionnel. De plus, nous avons démontré pour la première fois que l’application du correcteur du CFTR VRT-325 permettait, non seulement, la maturation du CFTR, mais également une amélioration de la capacité des monocouches de cellules des voies aériennes FK à se réparer. D’autre part, nous avons montré que la présence du filtrat bactérien de P. aeruginosa (PsaDM) altérait non seulement l’expression et la fonction du CFTR, mais également les processus de réparation épithéliale. Enfin, nos résultats montrent que l’infection affecte la maturation du CFTR induite par le VRT-325 et diminue les effets bénéfiques du VRT-325 sur la réparation épithéliale.
Mes travaux permettent de mieux comprendre le rôle du CFTR dans les processus de réparation de l’épithélium FK et de proposer une nouvelle approche thérapeutique visant à promouvoir la régénération épithéliale chez les patients FK afin de tenter de stabiliser leur état, malgré l’effet délétère de la composante infectieuse. / The Cystic fibrosis (CF) pathology is caused by mutations of the gene coding for the CFTR channel. The most common mutation is the deletion of Phe508 (∆F508) causing protein misfolding and degradation. Thus, the absence of CFTR channel causes the dysfunction of ion and fluid transport that impairs mucociliary clearance resulting in mucus thickening and plugging in the airways. These conditions then favor P. aeruginosa bacterial colonisation and inflammation in the airways, which contribute to airway damage and remodeling. Ultrstructural analysis of bronchial sections of lungs collected from CF patients at the time of transplantation showed some area with signs of ongoing epithelial repair. However, a progressive epithelial damage is still observed in CF patients and it appears that the repair process is insufficient to restore epithelial integrity.
The main objective of my work was to study the role of CFTR in the CF repair processes and to explore the impact of CFTR correction on epithelial repair under pathogen-free condition as well as in the presence of infectious products. Our study showed that CF airway epithelium exhibits a repair defect, associated, at least in part, to the absence of a functional CFTR channel. Furthermore, we demonstrated for the first time that CFTR rescue with the CFTR corrector VRT-325 significantly improved the wound-healing capacity of CF epithelial cell monolayers. Then, we showed that the presence of bacterial filtrate, more precisely P. aeruginosa diffusible material (PsaDM), not only alter CFTR function and expression, but also impaired epithelial repair processes. Finally, our results suggested that infection has a deleterious impact on CFTR rescue, and affected the beneficial effect on epithelial repair processes induced by VRT-325.
My work allows to better understand the role of CFTR in the CF epithelial repair mechanisms and to propose new therapeutic strategies to promote epithelial regeneration in CF patients in attempt to stabilize their condition, despite the deleterious impact of infection.
|
Page generated in 0.0905 seconds