Made available in DSpace on 2016-04-28T20:15:26Z (GMT). No. of bitstreams: 1 2007-Tiago Marques dos Santos.pdf: 2575604 bytes, checksum: e88af70c2740e40f39ed1fb8bd423ec5 (MD5) Previous issue date: 2007-02-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This work aimed to evaluate gastrointestinal and systemic disturbances, and mucosa and gut contents of gastrointestinal tract of horses subjected to overload carbohydrate. Eight crossbreed mature horses were used with body weight (BW) average of 364kg, geldings, adapted to diet composed by grass hay and concentrate, in a 60:40 proportion. A complete randomized design was used with horses allocated in three treatments. Treatment I: (Control) (n=2) horses were slaughtered without overload carbohydrate; Treatment II (n=3) and III (n=3), horses subjected to overload carbohydrate, with gastric infusion of 17.6 g starch/kg BW, and slaughtered after 24 and 36 hours, respectively. Horses were subjected to clinical, hematological and fecal evaluations before the overload and 2, 4, 8, 12, 16, 20, 24, 28, 32 and 36 hours after overload. Four hours after overload horses became depressed and keeping until the end of evaluation, and one horse presented lameness 36 hours after overload. Any difference (P>0.05) were observed in heart rate, respiratory rate, body temperature and hoof temperature. Increase in packed cell volume and plasma protein concentration were observed 24 hours after overload, varying from 26.7 to 32.0% and 7.1 to 8.1 g/dL, respectively (P<0.05). Differences were observed (P<0.05) in plasmatic lactate concentration in zero, 20 and 28 hours after overload, with values of 0.7, 1.04 and 1.22 mmol/L, respectively. Plasma endotoxin concentration didn't cross 0.1000 EU/mL and may be not present. There were any difference (P>0.05) in fecal and digesta water content, however, fecal pH reduced along 36 hours post-overload (P <0.01), varying from 6.09 to 4.46. Content of large intestine in horses subjected to overload presented whitish-green color, milk aspect, with gas bubbles and acid odor. There weren t difference (P>0.05) in water content of feces and digesta, however, fecal pH reduced along 36 hours post-overload (P <0.01), varying from 6.09 to 4.46. Buffering capacity of ceco-colon digesta and feces were reduced in horses subjected to overload. Right dorsal colon, transverse colon and descendent colon were segments, except stomach, that presented lower pH values, varying from 4.49 to 4.56. Eosinophils infiltration were presented in mucosa and submucosa of all horses, however, only horses submitted to overload presented neutrophils and eosinophils leucocitoestase with neutrophils predominance restricted to large intestine. Tract gastrointestinal circulatory alterations observed were congestion, edema and lymphatic vessels dilatation, more evident in submucosa, with larger inflammatory cells infiltration in horses subjected to overload. Intestinal mucosa 36 hours after overload presented larger degree of imunorreactivity anti-myeloperoxidase, followed by horses evaluated at 24 hours after overload and control horses, varying from 2.7 to 4.0, 1.0 to 3.7 and 1.0 to 2.5, respectively. Overload carbohydrate in horses promoted intensive fermentation in ceco-colon, predisposing clinical disturbances, digesta alterations and mucosa and submucosa lesions at gastrointestinal tract of horses with light to moderate degree,36 hours after overload. / Este trabalho teve como objetivo avaliar as altera??es sist?micas, da mucosa e conte?do do trato gastrointestinal de eq?inos submetidos ? sobrecarga diet?tica com amido. Foram utilizados oito eq?inos adultos castrados, com peso vivo m?dio de 364 kg, adaptados a dieta composta por feno de Coastcross e concentrado, na propor??o de 60:40. Foi utilizado um delineamento experimental com tr?s tratamentos: Tratamento I (Controle) (n=2), eutan?sia dos animais sem sobrecarga com amido; Tratamentos II (n=3) e III (n=3), animais submetidos ? sobrecarga com amido, com infus?o g?strica de 17,6 g amido/kg de peso corporal e eutan?sia ap?s 24 e 36 horas, respectivamente. Os eq?inos foram submetidos a avalia??es cl?nicas, hematol?gicas e f?sico-qu?micas das fezes antes da sobrecarga e 2, 4, 8, 12, 16, 20, 24, 28, 32 e 36 horas p?s-sobrecarga. Os animais apresentaram-se ap?ticos quatro horas ap?s a sobrecarga permanecendo assim at? o final da avalia??o e apenas um animal apresentou claudica??o, 36 horas p?s-sobrecarga. N?o houve diferen?a (P>0,05) na freq??ncia card?aca, freq??ncia respirat?ria, temperatura corporal e temperatura dos cascos. Houve aumento (P<0,05) no volume globular e prote?na plasm?tica total, 24 horas p?s-sobrecarga, variando de 26,7 a 32,0% e de 7,1 e 8,1 g/dL, respectivamente. Houve diferen?a (P<0,05) na concentra??o plasm?tica de lactato no tempo zero, 20 e 28 horas p?s-sobrecarga, com valores de 0,7, 1,04 e 1,22 mmol/L, respectivamente. A concentra??o plasm?tica de endotoxinas n?o ultrapassou 0,1000 EU/mL, podendo estar at? mesmo ausente. O conte?do do intestino grosso nos eq?inos submetidos ? sobrecarga apresentou cor verde esbranqui?ada, aspecto leitoso, com bolhas de g?s e odor ?cido. N?o houve diferen?a (P>0,05) no teor de ?gua das fezes e do conte?do da digesta, no entanto, o pH fecal reduziu ao longo de 36 horas p?s-sobrecarga (P<0,01), variando de 6,09 a 4,46. Houve redu??o na capacidade de tamponamento das fezes nos eq?inos submetidos ? sobrecarga e, de forma similar, ocorreu no conte?do do ceco-c?lon. O c?lon dorsal direito, c?lon transverso e c?lon descendente foram os segmentos, com exce??o do est?mago, que apresentaram a digesta com menores valores de pH, variando de 4,49 a 4,56. ? histopatologia, a infiltra??o de eosin?filos esteve presente na mucosa e submucosa de todos os eq?inos, no entanto, somente em dois eq?inos submetidos ? sobrecarga, observou-se leucocitoestase de neutr?filos e eosin?filos, com predomin?ncia de neutr?filos no intestino grosso. As altera??es circulat?rias observadas no trato gastrointestinal foram congest?o, edema e dilata??o de vasos linf?ticos, sendo mais evidentes na submucosa, local de maior infiltra??o de c?lulas inflamat?rias nos eq?inos submetidos ? sobrecarga. A mucosa dos segmentos do trato gastrointestinal dos eq?inos eutanasiados 36 horas p?ssobrecarga apresentou maior grau de imunorreatividade anti-mieloperoxidase, seguido dos eq?inos avaliados 24 horas p?s-sobrecarga e dos eq?inos do tratamento controle, variando de 2,7 a 4,0, 1,0 a 3,7 e 1,0 a 2,5, respectivamente. A sobrecarga diet?tica com amido em eq?inos promove fermenta??o intensa no ceco-c?lon, predispondo ao aparecimento de dist?rbios cl?nicos, altera??es do conte?do da digesta e les?es de leve a moderada na mucosa e submucosa do trato gastrointestinal dos eq?inos, ap?s 36 horas da sobrecarga.

Made available in DSpace on 2016-04-28T20:18:33Z (GMT). No. of bitstreams: 1 2006 - Marcelo de Oliveira Henriques.pdf: 1418850 bytes, checksum: 6ccdf4d93022fbed75fdf435f7413052 (MD5) Previous issue date: 2006-10-25 / This study was conducted with the purpose to evaluate the clinical, laboratory and epidemiological aspects of naturally acquired Babesia equi infection in 178 horses handled in a semi-intensive system, property of the Academia Militar das Agulhas Negras Brazilian Army, situated in the city of Resende, in the Rio de Janeiro State. Babesia equi is a species of hemoparasite of elevated prevalence among equids in tropical regions, being hard to control, causing important economic losses because of the long periods of convalescence, decline of performance in the affected animals, expenses with medication and workers, besides the restrictions in transit of equines, either for hippic competitions or international commerce. In the Indirect Immunofluorescence Reaction, a prevalence of 85,96% (n=153) was verified, therefore the area can be considered endemic for Babesia equi and in enzootic stability. There was no significant difference in the age or sex of the seropositive animals. Laboratory parameters like packed cell volume, red blood cell count, platelet count, global and differential white blood cell counts, dosages of seric creatinine, albumin and total plasmatic proteins, all of which led to the conclusion that in the assymptomatic carrier state there is no hematologic or seric biochemical variations that could be attributed to the protozoal Babesia equi. / Este estudo foi conduzido com o intuito de avaliar achados cl?nicos, laboratoriais e epidemiol?gicos na infec??o natural de Babesia equi em 178 eq?inos mantidos em sistema semiintensivo de cria??o, de propriedade da Academia Militar das Agulhas Negras Ex?rcito Brasileiro, situada no munic?pio de Resende, Estado do Rio de Janeiro. Babesia equi ? uma esp?cie de hemoparasita de elevada preval?ncia entre eq??deos de ?reas tropicais, sendo de dif?cil controle, causando perdas econ?micas importantes, pelos longos per?odos de convalescen?a, queda de desempenho nos animais acometidos, gastos com medicamentos e m?o de obra, al?m de impor restri??es no tr?nsito de eq?inos, seja para competi??es h?picas ou com?rcio internacional. Na Rea??o de Imunofluoresc?ncia Indireta, foi verificada uma preval?ncia de 85,96% (n=153), podendo ser a ?rea considerada end?mica para Babesia equi e em estabilidade enzo?tica, n?o sendo observadas diferen?as significativas entre sexo e idade dos animais. Par?metros laboratoriais como volume globular, hematimetria, plaquetometria, leucometrias global e espec?fica, dosagens de creatinina s?rica, albumina e prote?nas plasm?ticas totais foram conduzidos, podendo-se concluir que no estado de portador assintom?tico n?o h? varia??o hematol?gica ou de bioqu?mica s?rica que possa ser creditada ao protozo?rio Babesia equi.

Made available in DSpace on 2016-04-28T20:18:34Z (GMT). No. of bitstreams: 1 2009 - Carlos Alexandre Paula Goncalves.pdf: 1454724 bytes, checksum: 032bda4d175200a7de693f3e18dc6e4d (MD5) Previous issue date: 2009-08-21 / The goal of this study was identify whether the number of apoptotic cells in horse s lamellar tissues after carbohydrate overload-induced of laminitis were affected by non-steroidal anti-inflammatory. The TUNEL assay and morphological cells changes were used to identify cells that were dying by apoptosis. There was no significant difference between groups. The apoptotic rate was not affected by non-steroidal anti-inflammatories used after developmental phase of laminitis. / O objetivo deste trabalho foi identificar se a administra??o de anti-inflamat?rios n?o esteroidais influenciava no ?ndice apopt?tico de c?lulas epiteliais do tecido lamelar de cavalos com laminite induzida por administra??o de amido. O tecido lamelar foi coletado por bi?psia, fixado e corado pela t?cnica de TUNEL, utilizando-se igualmente a morfologia celular para identifica??o das c?lulas que estivessem em diferentes est?gios de morte por apoptose. N?o houve diferen?a significativa no ?ndice apopt?tico entre os diferentes grupos. Os antiinflamat?rios empregados aplicados na fase de desenvolvimento da laminite n?o influenciaram significativamente os ?ndices apopt?ticos.

Made available in DSpace on 2016-04-28T20:17:27Z (GMT). No. of bitstreams: 1 2007 - Antonia Regina Sessa da Silva.pdf: 384094 bytes, checksum: 91d2147f8402249dead112e338e89b66 (MD5) Previous issue date: 2007-03-30 / Brazil has currently the second bigger flock of equines of the world and the brazilian equines breeding plays an important role in the development of the sector of agribusiness. Among the infectious disease that affect the national equines breeding, the Equine infectious anemia (EIAV) has been shown of difficult control. The Equine infectious anemia is caused by a retrovirus, it has a worldwide distribution, and it is recognized as the most important disease of equines. The most common clinical signals in the acute phase, are anemia followed of jaundice in the mucosae, ventral oedema, mioglobinury, caquexy and, mainly, intermittent fever. Once it has no treatment, Ministry of Agriculture, Cattle and Supplying determines, through legal mechanisms, a politics of obligatory examination in credentialed laboratories, for the transport and commercialization of equines in the country. The examination is based on the test of Coggins, an immunodiffusion in agar gel (IDGA) of the serum of the animal tested against antigen of the EIAV. As the infection is lifetime, positive animals are euthanasiated by Veterinarians of the Ministry of Agriculture. Considering that precision of the results is critical, once that false positives animals could uselessly be sacrificed, and false negatives will be preserved as infection source, diverse problems may occur, appeared the interest of a systematic analysis of the reproducibility of kits and on possible sources of error. For this purpose, positive and negative sera, referred by the repetition of the tests for Coggins and ELISA, had been evaluated by IDGA comparatively with three kits of different manufacturers. In the experiments of reproducibility, it observed a great distinction in the quality of the precipitation line promoted for the positive sera in the tests. This phenomenon may be justified by the use of the conditions techniques legal, determined for would carry of the Ministry of Agriculture that is distinct of the recommendation of the analyzed manufacturers of two of kits. Exactly thus, the evaluation of the reproducibility of the results of kits with the referred sera showed high correlation. / O Brasil possui atualmente o segundo maior rebanho de eq??deos do mundo e a Eq?ideocultura Brasileira desempenha um importante papel no desenvolvimento do setor de agroneg?cios. Entre as doen?as infecciosas que afetam a eq?inocultura nacional, a Anemia Infecciosa dos Eq?inos (AIE) tem se mostrado de dif?cil controle. A AIE ? causada por um retrov?rus, apresenta uma distribui??o mundial, e ? reconhecida como a mais importante doen?a dos eq?inos. Entre os sinais cl?nicos mais comuns, na fase aguda da doen?a, encontram-se a anemia seguida de icter?cia nas mucosas, edema ventral, mioglobin?ria, caquexia e, principalmente, febre intermitente. Como n?o h? tratamento, Minist?rio da Agricultura, Pecu?ria e Abastecimento (MAPA) determina, atrav?s de mecanismo legais, uma pol?tica de exame obrigat?rio em laborat?rios credenciados, para o transporte e comercializa??o de eq??deos no Pa?s. O exame ? baseado na prova de Coggins, uma imunodifus?o em ?gar gel do soro do animal testado contra ant?geno do v?rus da AIE. Como a infec??o ? vital?cia, animais soropositivos s?o eutanasiados por M?dicos Veterin?rios do MAPA. Considerando que a precis?o dos resultados ? cr?tica, posto que animais falso positivos podem ser sacrificados inutilmente, e falso negativos preservados como fonte de infec??o, surgiu o interesse de um an?lise sistem?tica da reprodutibilidade dos kits e poss?veis fontes de erro no diagn?stico. Para esta finalidade, soros positivos e negativos, referenciados pela repeti??o dos testes de Coggins e ELISA, foram avaliados pela prova de IDGA, comparativamente com tr?s kits de diferentes fabricantes. Nos experimentos de reprodutibilidade, observou-se uma grande distin??o na qualidade da linha de precipita??o promovida pelos soros positivos nos testes nos diferentes kits. Este fen?meno pode ser justificado pela utiliza??o das condi??es t?cnicas legais, determinadas por portaria do MAPA, que s?o distintas das recomenda??es dos fabricantes de dois dos kits analisados. Mesmo assim, a avalia??o da reprodutibilidade dos resultados dos kits com os soros referenciados mostrou elevada correla??o.