Made available in DSpace on 2016-04-28T20:17:26Z (GMT). No. of bitstreams: 1 2006 - Rodrigo Alcantara de Carvalho.pdf: 803403 bytes, checksum: 7a35f34784a3f9cb58b545f52959a6a0 (MD5) Previous issue date: 2006-09-22 / The world wide contamination of foods and byproducts with mycotoxins is a problem of extreme importance. Mycotoxins are secondary metabolites produced by fungi that have adverse effects to human and animal health. The fumonisins and aflatoxins are the mycotoxins of greatest agro-economic importance due to the damage brought to animal production. Important steps in the synthesis of these mycotoxins are described, as such as their metabolism and adverse effects. This work evaluates the determination of the biomarkers sphinganin (Sa) and sphingosin (So) in poultry serum as a diagnose method for fumonisin exposition, and compares the obtained data with ration analysis. Of the evaluated animals only 49% exhibited 2 sphingolipids (Sa and So), 53,04% exhibited only So and 2,04% exhibited only Sa. It was detected at ration the presence of fumonisin B1 at concentration of 374,92μg.kg-1 and aflatoxins B1, B2, and G1 at concentrations of 440,99μg.kg-1, 169,92μg.kg-1 and 494,58μg.kg-1, respectively. It was possible to partially establish the grade of intoxication of the poultry and to verify that the sensibility of the biomarker can be affected by the presence of fumonisin such as aflatoxins. / A contamina??o mundial de alimentos e subprodutos com micotoxinas ? um problema de extrema import?ncia. As micotoxinas s?o metab?litos secund?rios produzidos por fungos, que possuem efeitos adversos ? sa?de humana e animal. As fumonisinas e aflatoxinas s?o as micotoxinas de maior import?ncia agro-econ?mica pelos preju?zos acarretados ? produ??o animal. Passos importantes na s?ntese destas micotoxinas s?o descritos, assim como seu metabolismo e efeitos adversos. Este trabalho avalia a determina??o dos biomarcadores esfinganina (Sa) e esfingosina (So) no soro de frangos de corte como m?todo de diagn?stico para exposi??o ?s fumonisinas e compara os dados obtidos com a an?lise da ra??o. Dos animais avaliados apenas 49% exibiram quantidades detect?veis de esfingolip?deos no soro, dentre estes, apenas 44,9% exibiram os 2 esfingolip?deos (Sa e So), 53,06% exibiram somente So e 2,04% exibiram somente Sa. Foi detectada na ra??o a presen?a de fumonisina B1 na concentra??o de 374,92μg.kg-1 e de aflatoxinas B1, B2 e G1 nas concentra??es de 440,99μg.kg-1, 169,92μg.kg-1 e 494,58μg.kg-1, respectivamente. Foi poss?vel estabelecer parcialmente o grau de intoxica??o dos frangos e verificar que a sensibilidade do biomarcador pode ser afetada tanto pela presen?a de fumonisina quanto de aflatoxina.