Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-07-25T18:12:15Z No. of bitstreams: 1 2016 - Marimar Mayworm Beck.pdf: 2648068 bytes, checksum: c6ec41428f0e812cecccc4dbfc94a8e9 (MD5) / Made available in DSpace on 2017-07-25T18:12:15Z (GMT). No. of bitstreams: 1 2016 - Marimar Mayworm Beck.pdf: 2648068 bytes, checksum: c6ec41428f0e812cecccc4dbfc94a8e9 (MD5) Previous issue date: 2016-08-31 / Man and animals are constantly subjected to stress, which is a situation caused by a challenge to which a body is subjected. Despite the exercise be a powerful and relatively simple way of treating and preventing diseases, acute exercise is considered an effective tool to study the physiological responses to metabolic stress. Like other forms of stress, acute exercise induces neuroendocrine and autonomic responses characterized mainly by activation of the hypothalamic-pituitary-adrenal axis and the sympathetic-adrenal system. Acupuncture is an ancient practice of Traditional Chinese Medicine, used to reduce stress responses in humans and animals. Therefore, the work suggests to study the effect of acute stimulation of acupuncture points on the physiological responses to progressive incremental exercise (PIE) in shelter dogs, by analysis of the variability of the cardiac interval (VIC), lactate and serum cortisol. It is noteworthy that the PIE is an exercise stress test recognized worldwide, that has been used in domestic animals and humans. The experimental procedures were performed in 16 dogs, mix breed, aged between 2 and 5 years, weighing 8-23 kg healthy with vaccinations and deworming updated and untrained to the treadmill. The dogs were subjected to an adaptation to the treadmil that consisted of 5 consecutive days of training 20 min each, with variable speeds (maximum of 5 km / h) and 5% inclination. On the day of the experiment the animals were divided into two groups: control (CTL only submitted to PIE) and acupuncture (treated with acupuncture before PIE). In ACUP group acupuncture needles were inserted into acupoints BP6, E36 and PC6 bilaterally for 20min. The PIE consisted in walking, followed by running in 5 km / h for 5 minutes, then gradually increase 0.5 km / h every 30 seconds, with inclination of 5%, until reaching exhaustion (abrupt stop or coordination loss during exercise). For the analysis of VIC, cardiac intervals were recorded by cardiac frequency meter (Polar); lactate and cortisol were analysed by spectrophotometer and radioimmunoassay, respectively. The EIP was able to significantly increase lactate levels, average HR, LF / HF ratio and LH and decrease the HF in the final three minutes of exercise demonstrating a greater activation of the sympathetic component in relation to the parasympathetic. However PIE was not able to induce significant increase of cortisol. No significant differences were detected between the acupuncture and control groups in any of the parameters analysed. We conclude that an acupuncture session immediately before the EIP was unable to significantly change the autonomic and endocrine responses induced by exercise. / Humanos e animais s?o constantemente submetidos ao estresse, que ? uma situa??o gerada por um desafio ao qual um organismo ? submetido. Apesar do exerc?cio f?sico ser uma forma poderosa e relativamente simples de tratamento e preven??o de doen?as, o exerc?cio agudo ? considerado uma ferramenta eficaz para estudar as respostas fisiol?gicas ao estresse metab?lico. Como outras formas de estresse, o exerc?cio agudo induz respostas neuroend?crinas e auton?micas caracterizadas principalmente pela a ativa??o do eixo hipot?lamo-hip?fise-adrenal e do sistema simpato adreno-medular. A acupuntura ? uma pr?tica milenar da Medicina Tradicional Chinesa, utilizada para reduzir as respostas de estresse em humanos e animais. Desta forma, o estudo prop?e estudar o efeito da estimula??o aguda de pontos de acupuntura nas respostas fisiol?gicas ao exerc?cio incremental progressivo (EIP) em c?es de canil, atrav?s da an?lise da variabilidade do intervalo card?aco (VIC), do lactato e do cortisol s?rico. Os procedimentos experimentais foram realizados em 16 c?es, sem ra?a definida, com idades entre 2 e 5 anos, pesando de 8 a 23 kg, clinicamente saud?veis, com vacinas e vermifuga??o em dia e destreinados ? esteira ergom?trica. Os c?es foram submetidos a um per?odo de adapta??o a esteira que consistiu de 5 dias de treino consecutivos de 20 min cada, com velocidades vari?veis (m?xima de 5 km/h) e 5% de inclina??o. No dia do experimento os animais foram divididos em dois grupos: controle (CTL, apenas submetidos ao EIP, n=8) e acupuntura (tratados com acupuntura antes do EIP, n=8). No grupo ACUP foram inseridas agulhas de acupuntura nos acupontos BP6, E36 e PC6 bilateralmente por 20min. O EIP consistiu de caminhada seguida de corrida de 5 km/h por 5 minutos, seguida de aumento gradual de 0,5 km/h a cada 30 segundos, com inclina??o de 5%, at? o alcance da exaust?o que foi considerada por meio de uma parada abrupta ou perda de coordena??o durante o exerc?cio. Para a an?lise da VIC, os intervalos card?acos foram registrados por frequenc?metro card?aco (Polar), e o lactato e cortisol analisados atrav?s de espectofot?metro, e pela t?cnica de radioimunoensaio, respectivamente. O EIP foi capaz de aumentar significativamente os n?veis de lactato, a FC m?dia, a raz?o LF/HF e o LH e diminuir o HF nos tr?s minutos finais do exerc?cio demonstrando uma maior ativa??o do componente simp?tico em rela??o ao parassimp?tico. No entanto o EIP n?o foi capaz de induzir aumento significativo do cortisol. N?o foi detectada diferen?a significativa entre os grupos acupuntura e controle em nenhum dos par?metros analisados. Conclu?mos que uma sess?o de acupuntura imediatamente antes do EIP n?o foi capaz de alterar significativamente as respostas auton?micas e end?crinas induzidas pelo exerc?cio.