In the first study 30 Hereford steers were fed a ration of either steam rolled barley or a 50:50 mixture of barley and alfalfa leaf meal pellets. In addition to these basal rations groups of animals on each, were fed a protein supplement with Diethylstilbestrol (D.E.S.) at levels such that each animal received either 0, 10, or 18 mgm. of D.E.S./head/day. The hormone treatment of animals fed the barley ration did not affect the growth rate or feed efficiency but a significant increase in hemoglobin levels from 8.96 to 10.40 gm. % Hb. and in the red cell counts from 8.25 million/mm³ to 9.50 million/mm³ resulted. A similar increase in red cell count from 8.7 million/mm³ to 9.6 million/mm³ resulted from hormone treatment of the animals fed the 50:50 barley-alfalfa ration. There was also an apparent, but insignificant, increase in haematocrits on both rations due to hormone treatment. This treatment resulted in a significant increase in the ratio of blood acetic to propionic acids on the barley ration from 82.3:1 to 195.3:1 but, did not cause a change in total blood volatile fatty acids (V.F.A.). There was an increase in the blood acetic-propionic ratio on the barley-alfalfa ration due to D.E.S. addition. This was from 97:1 to 159:1 at 10 mgm. D.E.S./head/day, to 233:1 at 18 mgm. of D.E.S./head/day but, was insignificant due to high within-group variability. There was an apparent difference between the three blood parameters (Haematocrit, Hemoglobin, Red Cell Counts) on the two control rations due to the higher iron content of the barley-alfalfa ration. This, and an apparent decrease in thyroid weights were shown to be insignificant. In the second study, using an all barley ration and a protein supplement containing a high iron concentration, there was a stimulation in growth rate and feed efficiency due to D.E.S. The animals were started on D.E.S. at 718 lb. (cf. 465 lb. for Study I). The increase in the three blood parameters was again observed on the low iron rations but, on the high iron ration there was an apparent but insignificant decrease in these parameters due to D.E.S. The animals fed the control high iron ration produced an haematocrit and red cell count that was significantly higher than that of the control low iron ration, indicating a possible deficiency in the original supplements. On the low iron ration there was a significant increase in total blood V.F.A. from 0.88 meq./l. to 1.19 meq./l. in response to D.E.S. supplementation but, on the high iron ration the increase was insignificant. The difference between the two control rations (0 D.E.S., high and low iron) total blood V.F.A. was shown to be significant, 0.88 vs. 1.08 meq./l. at P< .05. A method of preparation of feed and liver samples for analysis of their mineral content by atomic absorption spectrophotometry was developed. There was shown to be a significant increase in liver copper storage on the low iron ration with increased levels of D.E.S. The feeding of a high iron ration caused a significant decrease (P< .05) in the level of copper in the liver from 84 ppm. to 37 ppm. A slight but insignificant increase in liver iron levels on the high iron ration and through the treatment with D.E.S. was observed. / Land and Food Systems, Faculty of / Graduate

This study was designed to determine which seral stages of the regenerating coast forest are most efficient at converting radiant energy into energy available as seasonal deer foods, and if the most efficient stages are most intensively used by deer. Populations of Columbian black-tailed deer (Odocoileus hemionus columbianus Richardson) in coastal British Columbia have been reported to reach maximum levels soon after logging and slash-burning, and to decline as. succession advances. The efficiency of a deer range in producing food has been suggested as a factor influencing reproductive success and thus, population density. Seasonal forage preferences were determined, through rumen content analysis. Cover composition up to four feet in height, and summer and winter estimates of quantity, variety and nutrient quality of the important food species were obtained in different seral stages. These data were then related to the intensity to which deer utilized each seral stage, as indicated by the abundance of pellet groups. An early salal-catsear (Gaultheria-Hypochaeris) association develops three to five years after slash-burning. This type was preferred by deer during spring and early summer. Herbaceous plants formed 60 per cent of the spring-summer diet and were represented by more species, covered more surface area, and produced more available forage in this seral stage than in any other. A salal-Douglas fir (Gaultheria-Pseudotsuga) association develops 12 to 15 years after slash-burning. This type was preferred during autumn and winter. Shrub and coniferous species formed 70 to 80 per cent of the autumn-winter diet and were represented by more species, covered more surface area, and produced more available forage in this seral stage than in any other. In addition, the crude protein, mineral, and ash contents of the important evergreen foods were higher when eaten (autumn-winter) than at other times. Heaviest deer use occurred in the seral stages where these evergreens were most available. The nutrient content of key foods changed significantly with season, and there was evidence of selection by deer of the most nutritious plants available. Tests to demonstrate declines in nutrient levels as seral succession advanced between the fourth and fourteenth years were inconclusive, particularly since there is a likelihood of significant declines occurring in the initial four years. However, seasonal and successional changes in food quality are believed to be a factor influencing range selection. It is concluded that the numbers of deer within a logged unit of coast forest are affected by the efficiency at which food is produced. Numbers within the whole community are affected by the availability of ideal food-producing units for each season. A further hypothesis is suggested which states that sustained populations cannot be expected in a logged coast forest because seral succession will inevitably cause regression of range quality. / Science, Faculty of / Zoology, Department of / Graduate

The feeding of three different rations varying mainly in crude fat and crude fiber level at a high level of intake to pregnant and non-pregnant ewes had a number of effects on blood glucose, lactic acid, acetone plus acetoacetate, and total volatile fatty acid concentrations during the last three months of gestation for the pregnant animals (February, March, and April). There was no significant effect due to time on blood glucose level or on the level of blood ketone bodies, during the latter stages of pregnancy, for animals consuming high, medium or low roughage rations. The intramuscular injection of 1 mg./kg. body weight of growth hormone into pregnant sheep for 7 days during the last month of pregnancy, caused no significant effects on blood glucose, lactic acid, acetone plus acetoacetate, or total volatile fatty acids concentrations. / Land and Food Systems, Faculty of / Graduate

A study, comprised of five parts, has been carried out on seven adult, female, pastel mink. Metabolic responses to ambient temperature have shown that these mink cannot tolerate temperatures much above 29°C, but are not unduly disturbed by low temperatures around 0°C. Under sleeping conditions, and within their thermal neutral zone (25 ±2°C) , the three animals measured did not demonstrate an abnormally high metabolic rate. Under conditions of prolonged fast, these mink appeared to maintain normal activity, although they lost much bodyweight. The apparently high, daily, urinary, nitrogen excretion, when related to heat production, gave a value of 2.5 mg of urinary nitrogen per Calorie (A.D.E.). The effect of additions of fibre to a basal ration has shown an inverse relationship between this component and the time of passage of the feed. The apparent digestibility of the ash component in the ration was markedly decreased with increasing increments of dietary fibre, but that of the fat was only very slightly reduced. Under these experimental conditions, nitrogen retention appears to be always positive, possibly reflecting a constant demand for fur growth, and a constant loss of nitrogen in the form of ammonia. Maintenance energy (A.D.E.) requirements of these mink have been quantitated. The effects of a decrease in cage capacity and the additions of fibre to the ration appear to decrease the animal's energy requirements. The Caloric intake of a one kg mink is almost identical to that suggested by the N.R.C. Water intake, when related to energy (A.D.E.), is appreciably less than the 1 gm / Calorie of heat produced generally accepted for mammals. However, it is in close agreement with figures obtained for the cat and for the mouse. The addition of a non-nutritive bulk to the ration significantly increases the water intake when related to the A.D.E. of the ration / Land and Food Systems, Faculty of / Graduate

The Fraser delta tidal marshes are important for migrating and wintering ducks, in particular, Mallard, Pintail, Widgeon, and Green-winged Teal. Agricultural, residential, and industrial development threaten the tidal marshes with destruction and quality loss. In order to preserve and protect the most valuable areas, and perhaps improve the characteristics which attract ducks, it was considered necessary to determine areas of most value, and the environmental characteristics which could be improved. A study of the occurrence and food habits of ducks on the tidal marshes was considered to be the best means of determining the importance of each unit. The relative importance of the tidal marshes for loafing and feeding habitat was indicated from a synthesis of all available information on duck use of the entire delta area. Aerial censuses conducted throughout two winters provided data on the distribution and abundance of Mallard, Pintail, Widgeon, and Green-winged Teal on the foreshore and adjacent upland. It was found that an annually variable population of ducks was present from September until May, with the largest numbers occurring during fall and spring migrations. The tidal marshes attracted approximately one-half the ducks on the deltas— with the largest proportions occurring in September, early October, late March, April, and May.. All four census units of the tidal marsh attracted similar winter totals of ducks although the relative number at each unit varied throughout the winter. As all of the tidal marshes appeared important to ducks, the features of all of them were studied. Area was determined from aerial photographs, and topographical relationships were determined from known tide levels. Composition and distribution of vegetation was determined from line transects. Relative production of seeds was determined from line transects and seed samples. The tidal marshes were found to cover approximately 3,733 acres, sloping from the approximately thirteen foot to the seven foot tide level. A six to eighteen inch "drop-off", which usually occurred near the ten foot tide level, separated the tidal marsh into an upper and lower zone, each with a different vegetative composition. Fourteen plant species were found, of which five Cyperaceae species, Scirpus americanus , Carex lyngbyei , Eleocharis macrostachya , Scirpus paludosus , and Scirpus validus composed ninety-three percent of all plants. The first two species, dominant on the lower and upper zones respectively, formed seventy percent of all plants. Distribution appears to be determined by the degree of tidal flooding, the degree of local drainage, and possibly by undemonstrated differences in soil and water salinity. Seed production varied annually, and was related to the degree of tidal flooding. Scirpus validus and Carex Lyngbyei produced the most seeds, followed next by Scirpus americanus. Ducks were collected on the tidal marshes and adjacent agricultural areas. Analysis of their stomach contents revealed that Carex Lyngbyei, Scirpus validus and Scirpus americanus were the most important- tidal marsh food items. Polygonum lapathifolium and P. persicaria were the most important seed foods taken on the agricultural areas. Although Mallard, Pintail, and Green-winged Teal consumed mostly seeds, Widgeon consumed primarily green vegetation, including winter rye, Lolium sp., and several Gramineae species. A synthesis of the available information indicates that the tidal marshes were most important as loafing areas from October until January, while during the remainder of the period, from September until May, they were also important for the provision of food. The control of water levels, by diking and pumping, appears to be essential for the improvement and protection of the tidal marshes. / Science, Faculty of / Zoology, Department of / Graduate

In order to investigate the nutritional requirements of ewes during late pregnancy and early lactation, two groups of ewes were given 90 gms. Digestible Crude Protein (D.C.P.) during pregnancy and 225 gms. D.C.P./head/day during early lactation. During the last six weeks of pregnancy ewes in Group II, provided with maintenance + 100%, requirement of Digestible Energy (D.E.) made significantly greater liveweight gains (P<0.01) than those in Group I and fed maintenance + 50%, D.E. During early lactation ewes in Group I, provided with maintenance + 150%, D.E. lost significantly less weight (P<0.05) than those in Group II which were given maintenance + 100%, D.E. There were no significant differences in milk yield and milk composition between the two groups, however pre-weaning lamb growth in Group I was significantly greater (P<0.05) than that in Group II. Average daily gain of weaned lambs (weaned at 8 to 10 weeks of age) given pelleted rations containing 20%, protein was significantly greater (P<0.05) than those given 16%, protein but there were no significant differences between lambs fed 20% or 13% and between 16%, or 13%, crude protein. A higher proportion of single lambs in the group given 13% protein may have accounted in part for the better gain of this group. Results of the digestion trial with these lambs indicated that there were no significant differences (P<0.05) in the dry matter digestibility and the protein digestion coefficient of the three types of pellets but the nitrogen retention was greatest in the lambs fed the 20%, protein. The results of the second experiment conducted, to study the effect of non-protein nitrogen in the form of urea on milk yield and milk composition of ewes indicated that there were no significant differences in milk yield, milk composition and milk urea N level of the three groups of ewes fed equal amounts of supplemental nitrogen, during early lactation, as soybean, soybean + urea and urea alone. Ewes fed soybean or soybean + urea had significantly lower plasma urea nitrogen levels (P<0.05) than those supplemented with urea alone. There was no significant difference between the plasma urea nitrogen level of the former two groups of ewes. There were no significant differences in the pre-weaning growth of lambs nursing ewes fed on pellets containing soybean + urea and urea alone but the lambs from ewes fed soybean made significantly slower (P<0.05) gain than those from the other two groups. .The reason for this slower rate of gain is difficult to explain. Results of the trial conducted to investigate the use of urea nitrogen in early weaned lambs indicated that the lambs fed on pellets containing soybean made significantly greater gains (P<0.05) than those on pellets containing urea as the sole source of supplemental nitrogen. There were however, no significant differences in weight gain of lambs fed on pellets containing soybean or soybean + urea and between soybean + urea or urea alone. There were no significant differences between plasma urea nitrogen level of lambs fed on soybean or soybean + urea containing pellets and these lambs had significantly higher (P<0.05) levels of plasma urea nitrogen than those fed on pellets containing urea alone. The variation in the blood urea level of the lambs was thought to be due to variation in protein intake. Results of the digestion trial with lambs showed that there were no significant differences in the protein digestion coefficients and dry matter digestibilities of these types of rations. Pellets providing nitrogen from soybean resulted in greatest nitrogen retention. Results show that urea did not influence the milk yield or milk composition of the ewes but it was a poor source of nitrogen for early weaned lambs. / Land and Food Systems, Faculty of / Graduate

The present study consisted of three sections each of which was undertaken to obtain information on some aspect of protein quality evaluation by biological testing. The first study consisted of two experiments in which the free amino acids were determined in the plasma of birds fed cereal diets to determine if this method would indicate the amino acid adequacy of the diets. The second study was an investigation of the use of chicken muscle meal as a reference supplementary protein. The third study consisted of an investigation of the fractionation of intestinal nitrogen as a method for studying protein quality. In the first series of experiments in which plasma free amino acids were determined to investigate protein quality two groups of White Leghorn chicks each were fed a control diet consisting of a high protein wheat diet supplemented with 0.24% lysine and 0.06% methionine and the control diet further supplemented with 0.1% L-arginine and glycine and 0.045% L-leucine and L-threonine. Blood samples were collected from birds on each diet after a 14 hour fast and after 3 hours and 6 hours of ad libitum feeding following the fast. The PFAA patterns obtained at both 3 and 6 hours after commencement of feeding from chicks which had been fed these two diets for three weeks indicated a lysine deficiency. The 6 hour plasma pattern from chicks fed the supplemented diet showed a greater percentage of threonine and less of the other EAA except arginine in comparison with the pattern seen in the plasma from birds fed the control diet. Lysine was shown to be the first limiting amino acid in diets containing 10.7% crude protein (N x 6.25) from wheat and 4% crude protein from rapeseed or soy bean meal by growth test and PFAA patterns. After 3 hours of ad libitum feeding different levels of circulating lysine were observed in the plasma from birds fed the rapeseed diets supplemented with 0.15% lysine. The levels of circulating lysine were not significantly different due to sample variation, but in general the higher levels of plasma lysine were seen in birds with the heavier body weights. A single level of supplementation was inadequate to determine the next limiting amino acid in these diets. In an investigation of the use of chicken muscle meals as a reference supplementary protein various chicken muscle meals were compared to an isolated soy protein and methionine reference supplement. The meals were prepared by freeze drying either cooked or raw tissue. One meal was treated with antioxidant. Diets containing 8% protein from the protein supplement and 10% protein from the wheat basal diet supported as good growth as that obtained with a good quality commercial fishmeal when tested at the same level of supplementation. All the chicken meals and the fishmeal supported better growth than the ISP and methionine reference control. A few small trials to investigate the use of gut nitrogen levels for evaluating protein quality were carried out. Preliminary trials included the determination of the nitrogen recoverable after the administration of different test meals with different protein sources, carbohydrates and fiber. Results obtained after administering single test meals were not considered reliable. A method was used where by the intestinal contents were sampled one-half hour after several meals had been administered and the nitrogen fractionated into TCA soluble and insoluble portions. When the amount of recoverable nitrogen insoluble in TCA was taken as the criterion of quality, differences were demonstrated between a sample of good quality protein supplement and one of extremely poor quality. Less of the nitrogen recovered from birds fed a high percentage of fiber in the test meals was soluble in TCA than when the protein was fed with corn starch or glucose mono-hydrate. The amount of liquid in the slurry was found to affect the amount of nitrogen recoverable if several feedings were administered in a relatively short period of time. Once the biological method was established preliminary determinations of the amounts of recoverable free basic amino acids from the intestinal segments were carried out. Generally a greater number of micromoles of basic amino acid per segment were found in the jejunum than in the ileum. In some cases there was less of these amino acids present in the gut of birds fed protein with glucose monohydrate than from the gut of birds receiving other treatments but this may only have been due to the effect of the liquid in the slurry. It was recommended that pellets would be a more biologically accurate method to administer the test meals. / Land and Food Systems, Faculty of / Graduate

A study was made of the effects of feeding excess vitamin e to chicks. Three groups of experiments were conducted to investigate (1) thyroidal response to excess vitamin e, (2) respiration rate of muscle mitochondria from chicks fed excess vitamin e; and (3) symptoms of hypervitaminosis e. In two separate experiments, chicks were fed normal and excess levels (220 i.u./kg. of diet) of vitamin e and were subjected to temperatures of 14.5 and 31.5°c. the goitrogenic effect of thiouracil on the birds in the different treatments was measured. At both temperatures the thiouracil-treated chicks fed excess vitamin e exhibited a lesser enlargement of the thyroid glands than did chicks receiving a normal level of vitamin e. This finding indicates a reduction in the secretion of thyroid stimulating hormone (tsh) in birds fed excess vitamin e. the level of vitamin e which was fed did not affect the growth rate or feed consumption at either temperature. It, therefore, appears that the metabolic rates of the chicks fed the low and excess levels of vitamin e were similar despite differences in thyroid activity and that tissue respiration in birds fed excess vitamin e can be maintained with a reduced supply or turnover of thyroid hormone. The activity of the thyroid gland itself was studied in response to excess vitamin e in another experiment. Using radioiodine (l¹³¹) as a tracer, it was found that the rates of iodine uptake and release by the thyroid gland were both slower in birds fed excess vitamin e (220 i.u./kg. 0f diet) than in control birds. Because vitamin e acts as a biological antioxidant, it may, in excess amounts, depress the rate of oeiodination of thyroxine in the peripheral tissue at which thyroid hormone is removed from the circulation. As a consequence, the secretion of tsh would be reduced. In order to obtain some indication of cause and effect in the mechanism by which the excess vitamin e affects thyrotropic hormone secretion rate and thyroid activity, the respiration rate of mitochondria isolated from the pectoral muscle of chicks which had been fed excess vitamin e (2200 i.u./kg. 0f diet) was compared with that of the control birds. The results showed a significant reduction in oxygen uptake by the muscle mitochondria of chicks fed the excess amount of vitamin e. The growth rate of the chicks fed the excess level of vitamin e in this experiment was markedly lower than that of the control chicks. It is suggested that the chick may maintain a normal respiration rate when fed excess vitamin e up to a certain level through a reduction in thyroid activity. With a large excess of vitamin e, however, no further compensation is possible. and respiration rate is depressed below normal. Excess vitamin e caused hypoprothrombinemia, indicative of vitamin k-deficiency. Other symptoms noted were a reduction in hematocrit values, reticulocytosis and an abnormally fluid appearance of the bone marrow. Based upon observations of bone calcification, the calcium requirement appeared to be increased in the presence of excess vitamin e. / Land and Food Systems, Faculty of / Graduate

The effects of two applications of foliar sprays made from kelp (Macrocystis intearifolia Bory) on growth of beans (Phaseolus vulgaris L.) under three moisture regimes were investigated. Date of kelp harvest had little effect on plants grown in water excess or deficit conditions, although means for kelp collected in spring tended to be higher. On soils maintained near field capacity, spray made from kelp collected in July resulted in increases in some bean yield components beyond the effects of kelp collected in spring or fall. The effect of storage of liquefied kelp at room temperature was also investigated and found to be soil moisture dependent. Leaf and root weights of plants grown in field capacity and dry soil respectively were lower for plants treated with kelp aged for 118 days compared to plants treated with kelp stored for longer or shorter durations. In wet soil conditions, the effect of sprays on plant height was reduced by storage of the liquefied kelp. Kelp extracts were fractionated by solvent partition. The butanolic fraction was shown to increase significantly many yield variables under wet soil conditions. This fraction had cytokinin-like activity in two bioassay systems, and contained substances co-eluting with isopentenyl adenine and zeatin in column, thin layer, and gas-liquid chromatography systems. Non-significant increases for some plant yield components resulting from application of unfractionated kelp paralleled these increases. A kelp fraction with undefined constituents increased some yield variables of plants grown in field capacity soil, as did a fraction with auxin and gibberellin-like activities in bioassay. The presence of inhibitors in the kelp was indicated by significantly lower pod yields under dry soil conditions, and a reduction in cytokinin-like bioassay activity when increasing amounts of kelp were assayed. The possibility that phytohormone deficits resulting from root stress are ameliorated by some components of the kelp spray is discussed. / Land and Food Systems, Faculty of / Graduate

Secretoneurin (SN) is a 31-43 amino acid, functional peptide derived by proteolytic processing from the middle domain of the ~600 amino acid secretogranin-II (SgII) precursor. In teleosts there are 2 forms arising from 2 different genes, SgIIa and SgIIb. In turn, there are both SNa and SNb in teleost. Secretoneurin is a well-conserved peptide during evolution from fish to mammals and widely distributed in secretory granules of endocrine cells and neurons. Secretoneurin plays important roles in different biological processes, for example controlling vertebrate reproduction by stimulating luteinizing hormone release from the pituitary. A potential new role of SN in feeding in goldfish is the subject of the research presented in this thesis. Firstly, we looked at the distribution of SgIIa mRNA in various female goldfish tissues using both RT-PCR and Q-PCR techniques in order to determine which tissue expresses SgIIa mRNA and in which level. We found that SgIIa mRNA was detected in different amounts in all tissues examined. The main tissues of interest were hypothalamus, telencephalon and gut, they all expressed SgIIa. Secondly, we examined the effect of acute (26 h), short (3 days), medium (7 days) and long (14 days) fasting and periprandial changes on SgIIa mRNA level in hypothalamus, telencephalon and gut using Q-PCR method. The results showed that SgIIa mRNA increases under the effect of acute and short fasting, however, medium and long fasting did not affect SgIIa mRNA. Thirdly, we examined the effect of brain injection of goldfish SNa on food intake and locomotor behavior and the expression of some feeding neuropeptides such as neuropeptide Y, orexin, chocystokinin and cocaine-and amphetamine-regulated transcript I after treatment. Injection of SNa in the third brain ventricle increased food intake and fish activity. Associated with this was an increase in NPY and decrease in CARTI mRNA levels in hypothalamus. The increase in SgIIa mRNA following fasting and the increase of food intake as a result of SNa treatment suggest a novel role for SNa in feeding processes.