The relationship between biodiversity and ecosystem functioning is important in maintaining agroecosystem sustainability. Plant-microbe symbioses, such as exists between the grass tall fescue (Schedonorus arundinaceum) and the asexual fungal endophyte Epichloë coenophiala, can be utilized to enhance agroecosystem functions, such as herbivore resistance. “Novel” E. coenophiala strains that vary in the production of mammal- and insect-toxic compounds have been identified, inserted into tall fescue cultivars, and are planted in pastures globally. Novel fungal endophyte-tall fescue associations may have divergent ecosystem function effects. This study assessed effects of different fescue-endophyte symbiotic combinations on pasture ecosystem function, including aboveground (fescue biomass, plant species richness, alkaloid synthesis, arthropod abundance) and belowground (soil microbial biomass, soil enzyme activity, trace gas fluxes) parameters. Results showed no significant effects of increasing symbiotic diversity within a fescue stand on aboveground measurements, bar arthropod abundance and alkaloid synthesis. Most soil parameters quantified had significant symbiotic diversity effects. For example, soil microbial biomass decreased whereas soil enzyme activity increased with increasing symbiotic diversity. Overall, our results suggested that increasing symbiotic diversity had weak to moderate effects on aboveground processes and stronger effects on certain belowground processes, indicating that symbiotic diversity can impact ecosystem functions and warrants further research.

Chewings fescue [Festuca rubra L. subsp. fallax (Thuill.) Nyman] is a desired turfgrass with dense sod forming capabilities and superior shade tolerance. Thermal residue management (open-field burning) has traditionally been used to remove post-harvest residue and maintain seed yield over the life of the stand. However, alternative non-thermal residue management practices have been observed to produce adequate seed yields dependent upon cultivar. Strong creeping red fescue (F. rubra L. subsp. rubra) is desired for its prolific tillering capacity and creeping rhizomatous growth habit. In contrast to Chewings fescue, maintenance of seed yield in strong creeping red fescue has only been profitably produced under thermal residue management. Slender creeping red fescue [F. rubra L. var. littoralis (Vasey)] is a desired turfgrass with a compact, less rhizomatous growth habit, similar to Chewings fescue in desirable turf attributes. However, little is known about the effects of post-harvest residue management in slender creeping red fescue. The objectives of this study were: 1) to evaluate seed yield and yield components among different cultivars to thermal (open-burning), and non-thermal (flail low and flail high) post-harvest residue management; 2) to evaluate harvest index and percent cleanout to thermal and non-thermal residue management in different cultivars; and 3) and to provide an economic analysis of thermal and non-thermal residue management in all cultivars based on partial budgeting. Three post-harvest residue management treatments (burn, flail low and flail high) were applied over the course of two years. Seed yield components measured included: total dry weight, fertile tiller number, spikelets per panicle, florets per spikelet, and panicle length. Final seed yield in each cultivar and residue management treatment method was determined after seed harvest and conditioning. Seed yield component analysis was conducted over three production seasons. Chewings fescue, strong creeping red fescue, and slender creeping red fescue cultivars responded differently to residue management as indicated by a residue management by cultivar interaction. In 2003 and 2004, residue management by cultivar interactions were evident in seed number, seed weight, fertile tiller number, percent cleanout, harvest index, and seed yield. Residue management by cultivar interactions occurred in spikelets per panicle in 2003, whereas in 2004 a residue management by cultivar interaction occurred in panicle length and florets per spikelet. In 2004, non-thermal flail low, and thermal residue management resulted in significantly greater spikelets per panicle in all cultivars. Thermal residue management resulted in the greatest number of spikelets per panicle. Results indicate that thermal residue management best maintained seed yield in most subspecies and cultivars across both years. However, in 2003, non-thermal flail low residue management produced profitable seed yield in only Marker slender creeping red fescue. In contrast, thermal residue management resulted in poor seed yields in Marker slender creeping red fescue and enhanced yields in Seabreeze slender creeping red fescue in 2003. However, following the second year of thermal treatment in 2004, Marker and Seabreeze both had lower seed yields, thus exhibiting the only negative impact of thermal management among the cultivars tested in this study. Moreover, upon review of an economic analysis, Marker slender red fescue was the only cultivar that produced a positive net return of $78 and $4 ha⁻¹ under non-thermal residue management in 2003 and 2004, respectively. Furthermore, in 2003, thermal residue management net return increases ranged from $104 ha⁻¹ to -$996 ha⁻¹ in Barnica and Shademark, respectively. In 2004, thermal residue management net return increases ranged from $115 ha⁻¹ and $1,332 ha⁻¹ in Seabreeze and Shademark, respectively. Poor seed yields were observed in all strong creeping red fescue cultivars under non-thermal residue management across both years of the study. This may be attributed to an observed reduction in fertile tiller number and seed yield. In addition, percent seed cleanout was increased with non-thermal residue management. In 2004, as stand age increased, thermal residue management resulted in greater seed yields in all cultivars and species, except both cultivars of slender creeping red fescue. Thus, this study provided substantial evidence that thermal residue management has the potential to maintain or increase fine fescue seed yield as stands age as well as to maintain stand profitability. / Graduation date: 2005

Ergovaline is produced by the endophyte Neotyphodium coenophialum (Morgan-Jones and Gams) in tall fescue (Schedonorus arundinacea (Schreb.) Dumort. = Festuca arundinacea Schreb.) and is blamed for a multitude of costly livestock disorders. Testing of pastures is common in both research and on farm situations. Since ergovaline is known to be unstable and affected by many variables, the objective of this study was to determine the effect of sample handling and storage on the stability of this compound. Homogeneous milled tall fescue sub-samples were analyzed for ergovaline concentration using HPLC after a range of sample handling procedures or storage. Ergovaline was unstable in milled material after 24 hours in storage, regardless of temperature. The decrease in ergovaline after 24 hours ranged from 17 to 60%. These results show that tall fescue sample handling and storage have a significant effect on ergovaline concentrations. In conclusion, accurate laboratory analysis of ergovaline content may require that samples be transported immediately to the laboratory on ice for immediate analysis. Most laboratories are not equipped for same day analysis, therefore researchers and producers should acknowledge that laboratory ergovaline results may be lower than the actual content in the field.

Tall fescue is a widely used forage grass in the eastern USA and can form a symbiosis with a fungal endophyte, which can be beneficial for the plant but can cause livestock health issues. Little is known regarding the symbiotic response to predicted climate change. To address this knowledge gap, I analyzed tall fescue variety trial data collected throughout the U.S., exploring relationships between climate variables and yield for two different fescue cultivars that were either endophyte-free or infected. This study showed no endophyte or cultivar effect on fescue yield, but identified temperature, precipitation and location as significant predictors of yield, suggesting that local conditions were more important than endophyte presence or fescue genotype for this dataset. Using a field experiment located in central Kentucky, I quantified the ecophysiological responses of four tall fescue genotypes to endophyte presence, elevated temperature and increased growing season precipitation. In this study, tall fescue genotype was as important as endophyte presence in determining ecophysiological responses to climate change treatments. My thesis illustrates that tall fescue response to climate change will depend on host genetics, the presence and genetics of the fungal endophyte symbiont, and the specific changes to the environment experienced at a site.

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<p dir="ltr">Water scarcity and acute drought continue to be serious concerns, even in humid climates where precipitation normally exceeds evapotranspiration (ET), highlighting the need for improved landscape water conservation practices. Lawns represent the largest area in most managed landscapes and require regular mowing, feeding and sometimes supplemental summer irrigation to persist. A general historical guideline for lawn irrigation is to supply 25-38 mm per growing wk-1 in the absence of rainfall. This fixed volume is often applied on a programmatic “set and forget” three times per week schedule (e.g. M-W-F). This application guideline often results in excess irrigation as it does not take into account plant need or prevailing environmental conditions. Further, in times of acute summer drought policymakers may restrict irrigation to once or twice weekly to conserve water. The effects of these regulations on turf health and potential water savings are unclear. Thus, two multi-year summer field studies were conducted to identify opportunities for improved lawn water conservation. Study one evaluated the effect of varying weekly irrigation volume and frequency on mature Kentucky bluegrass (Poa pratensis L.:KBG) located under a fixed-roof rainout structure. Turfgrass response was measured as visual turf quality (TQ), digital green color (DGC), volumetric soil water content (VWC) and area under the curve for each response variable. In year one, KBG was subject to six irrigation programs and compared to a high deficit control for 49 days. The programs were: 25 mm total water wk-1 applied either three times (M-W-F) or twice (M and F) weekly, 19 mm total wk-1 twice weekly (M and F), 13 mm total wk-1 once weekly and 60 or 80% accumulated ETo three times weekly. Due to poor performance, the high deficit control and 60% ETo were excluded from further evaluation. In year two, seven programs were evaluated: 33 mm total water wk-1 applied once, twice, or three times weekly, or every-other-day, 25 mm total wk-1 applied twice or three times weekly and 80% ETo twice weekly for 63 days. In both years, generally, 25-33 mm wk-1 applied two or three times wk-1 produced the most consistent TQ, DGC and highest VWC. Study two compared three supplemental irrigation programs to a natural rainfall control for six widely planted cool-season lawn grasses for 70 days. The six lawn grasses were: a KBG sod, seeded drought tolerant and susceptible KBG cultivars, a turf-type tall fescue (Schedonorus arundinaceus (Schreb.) Durmort.: TTTF) blend and two KBG and perennial ryegrass (Lolium perenne L.:PRG) mixtures. In Study two, highly significant differences p < .001 occurred for irrigation program and lawn grasses. While supplemental irrigation aided all grasses, rainfall in year one and two measured 119 mm and 343 mm, respectively, affecting data in each study year. Area under the turfgrass quality curve (AUTQC) was lowest for the rainfall control (339 and 425) and highest for 33 mm wk-1 applied three times (507 and 526) and 80% ETo programs (508 and 535) in year one and two, respectively. Annual responses for DGC and VWC generally followed TQ trends. For grasses subject only to natural rainfall, TTTF, and the KBGs were generally superior to the KBG:PRG mixtures, thus emphasizing the importance of species selection. In summary, these studies compared a traditional “set and forget” irrigation program to a range of alternative programs and different lawn grasses. These data provide evidence for irrigation savings by employing a combination of planting drought tolerant lawn species and adopting a more limited irrigation program. Future field studies should include the evaluation of adaptive, data-driven programs based on forecast environmental conditions and threshold responses like DGC or predetermined VWC set points calibrated for varying soil types and growing environments.</p>

A greater than 10-fold increase in Canada goose (Branta canadensis) populations over the past several years has resulted in concerns over grazing impacts on grass seed production in the mid-Willamette Valley, Oregon. This study was designed to develop methods to quantify and statistically analyze goose-grazing impacts on seed yields of tall fescue (Festuca arundinacea Schreb.) and perennial ryegrass (Lolium perenne L.). Yield-mapping-system equipped combines, incorporating global positioning system (GPS) technology, were used to measure and map yields. Image processing of ground-level photography to estimate crop cover and other relevant observations were spatially located via GPS to establish spatial-temporal goose grazing patterns. We sampled each field semi-monthly from mid-winter through spring. Spatially located yield data, soils information, exclosure locations, and grazing patterns were integrated via geographical information system (GIS) technology. To avoid concerns about autocorrelation, a bootstrapping procedure for subsampling spatially contiguous seed yield data was used to organize the data for appropriate use of analysis of variance. The procedure was used to evaluate grazing impacts on seed yield for areas of fields with different soils and with differential timing and intensity of goose grazing activity. We also used a standard paired-plot procedure, involving exclosures and associated plots available for grazing. The combination of spatially explicit photography and yield mapping, integrated with GIS, proved effective in establishing cause-and-effect relationships between goose grazing and seed yield differences. Exclosures were essential for providing nongrazed controls. Both statistical approaches were effective in documenting goose-grazing impacts. Paired-plots were restricted by small size and few numbers and did not capture grazing impacts as effectively as comparison of larger areas to exclosures. Bootstrapping to subsample larger areas of yield for comparison was an effective method of avoiding autocorrelation of data while better representing impacts within a field. Occasional yield increases, ranging from 1 to 5 percent, were recorded following goose grazing. Goose grazing generally resulted in seed yield reductions, ranging up to 20 percent. Later and more intensive grazing tended to increase yield reductions. Newly seeded tall fescue tended to be the most sensitive to grazing. Established perennial ryegrass tended to be more resilient. / Graduation date: 2003

Open-field burning has been an effective, economical, and widespread method of post-harvest residue management in creeping red fescue seed production in the Willamette Valley since the late 1940s. However, the use of field burning has been legislatively restricted due to air quality and safety issues. The foliar-applied plant growth regulator trinexapac-ethyl (TE), commercialized in the USA as Palisade, has been accepted by producers as a yield enhancing agent and is considered here as an alternative to open-field burning over a four-year period. The effects of open-field burning versus mechanical removal (flailing) of post-harvest residue, and spring versus fall applications of TE on seed yield, dry matter partitioning, and seed yield components were evaluated in a split-plot design. The response to the different treatment combinations differed across years. The young stand responded with a seed yield increase to spring TE applications, regardless of residue management treatment. However, as the stand aged, field burning became critical for maintaining high yields and, in 2003 and 2004, only spring TE applications resulted in seed yield increases in burned plots. The higher potential seed yield achieved in burned plots over flailed plots, as a result of a higher number of panicles per unit area and spikelets per panicle, was critical for maintaining high seed yields as the stand aged. Spring applications of TE, further increased seed yield over the untreated check by increasing the number of florets per spikelet, reducing fertile tiller height and lodging and consequently, favoring pollination and fertilization of the florets. Late spring TE applications also increased 1000-seed weight in 2003 and 2004. Although spring applications of TE were a promising alternative to open-field burning early during the life of the stand, as the stand aged they did not increase seed yield on flailed plots. Fall TE applications did not have a consistent effect on seed yield, dry matter partitioning or seed yield components, and were found not to be a viable management practice. / Graduation date: 2005

The hypothesis for this study was that phenanthrene degrading bacterial inoculants, in combination with grass species able to tolerate petroleum hydrocarbon contamination, will result in increased degradation, as compared to natural rates of hydrocarbon degradation, or to rates of degradation attributed to bacteria or plants alone. Three experiments were performed to examine this hypothesis: i) assessment of the effect of phenanthrene degrading bacteria (<i>Sphingomonas yanoikuyae</i>, <i>Rahnella aquatilis</i>, and <i>Arthrobacter globiformis</i>) on seed germination, location of attachment on seeds and roots, and inoculant survival on selected grass species, ii) determination of the inoculant survival in contaminated soil in the absence of plants and the ability to degrade target compounds, and iii) degradation potential and survival of selected grass species and bacterial inoculants in soil. In general, all applied inoculants were able to effectively colonize the seeds and had a neutral or positive effect on seed germination and seedling growth. Possible plant and bacteria pairs were chosen based on positive influence of the inoculant and are as follows: perennial ryegrass (<i>Lolium perenne</i>) or creeping red fescue (<i>Festuca rubra</i>) with <i>A. globiformis</i> or <i>S. yanoikuyae</i>, or slender wheatgrass (<i>Elymus trachycaulus</i>) with <i>A. globiformis</i> or <i>R. aquatilis</i>. Soil-based assessment of the survival and degradation of hydrocarbons by the selected inoculants was examined with or without a manure nutrient amendment. The addition of the inoculants had a positive impact on the efficacy of hydrocarbon removal in the soil. The manure-amended soil, or <i>A. globiformis</i> inoculated non-amended soil treatments reduced total petroleum hydrocarbon concentration by ~45%, whereas the non-amended control only resulted in a ~20% reduction. When soils were amended with manure and inoculated with any of the phenanthrene degrading bacteria, contaminant concentration decreased in soil by ~33%. <i>Sphingomonas yanoikuyae</i> survived the longest in soil in the absence of plants. A growth chamber experiment was conducted to determine the efficacy of plant and bacteria pairs for hydrocarbon removal in recalcitrant contamination found in soil from Bruderheim, Alberta. Additional replicates containing this soil were spiked with hexadecane, phenanthrene, and pyrene so the effectiveness of the plant and bacteria pairs at higher levels of fresh contamination could be assessed. In the spiked treatment, inoculation with <i>S. yanoikuyae</i> increased creeping red fescue root biomass. In the non-spiked treatment, <i>S. yanoikuyae</i> application increased creeping red fescue root and shoot biomass. Perennial ryegrass root and shoot biomass did not increase when inoculated with <i>S. yanoikuyae</i>, although root biomass values were observably higher in non-spiked soils. Creeping red fescue inoculated with <i>S. yanoikuyae</i> resulted in the greatest decrease in hydrocarbon concentration as compared to other treatments (~61%). The perennial ryegrass treatment, when inoculated with <i>S. yanoikuyae</i> increased percent hydrocarbon removal (~10%) above that obtained with perennial ryegrass alone. The addition of plants and <i>S. yanoikuyae</i> increased hydrocarbon degradation relative to control soils, although the addition of vegetation alone had a comparable effect. A critical benefit of inoculation was the increase in creeping red fescue root biomass at higher concentrations of contamination. This is important because the larger the root biomass the larger the volume of soil that can be remediated. The results indicate that the use of specific plant-bacterial inoculants can enhance remediation of hydrocarbon contaminated soils.