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Transplantace limbálních kmenových buněk a jejich využití k rekonstrukci povrchu oka / Limbal stem cell transplantation and their utilization for ocular surface reconstruction.Lenčová, Anna January 2015 (has links)
Aims: Limbal stem cell (LSC) deficiency is one of the most challenging ocular surface diseases. The aim of this thesis was to study damaged ocular surface reconstruction. Therefore, a mouse model of limbal transplantation was estab- lished. Furthermore, LSC isolation, transfer of LSCs and bone marrow-derived mesenchymal stem cells (MSCs) on nanofiber scaffolds were studied. Material and methods: Syngeneic, allogeneic and xenogeneic (rat) limbal grafts were transplanted orthotopically into BALB/c mice. Graft survival, immune re- sponse and the effect of monoclonal antibodies (mAb) (anti-CD4 and anti-CD8 cells) were analyzed. Mouse LSCs were separated by Percoll gradient; subse- quently, they were analyzed for the presence of LSC and differentiation corneal epithelial cell markers and characteristics using real-time PCR and flow cytom- etry. Nanofiber scaffolds seeded with LSCs and MSCs were transferred onto the damaged ocular surface in mouse and rabbit models. Cell growth on scaffolds, post-operative inflammatory response and survival of transferred cell were ana- lyzed. Results: Limbal allografts were rejected promptly by the Th1-type of immune response (IL-2, IFN-γ) involving CD4+ cells and nitric oxide produced by macro- phages, contrary to the prevailing Th1 and Th2 immune responses (IL-4, IL-10) in...
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Cultivo org?nico de tomate em vasoponia e ambiente protegido com manejo da irriga??o por acionador autom?tico / Organic cultivation of tomatoes in potponics and greenhouse with irrigation management by controller automaticGOMES, Daniela pinto 26 February 2016 (has links)
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Previous issue date: 2016-02-26 / CAPES / FAPERJ / This work entitled "Organic farming tomatoes in potponics and greenhouse with irrigation management by controller automatic" aimed to provide information to serve as a recommendation for a new tomato cultivation system potted and greenhouse with respect to inputs water and fertilizer, to ensure a sustainable production, mainly based on the use of residu?rios materials as a source of nutrients and automatic irrigation low cost. The specific objectives of this study were to assess: (a) the effect of different doses of wood ash and castor bean cake in the growth and productivity of tomato culture; and (b) the effect of different irrigation levels on growth and productivity of the tomato crop. Three experiments were conducted from 2013 to 2015 using a randomized block. In 2013, we evaluated the effect of five crecentes doses of wood ash and castor bean cake (160 g of ash and 90 g of castor bean cake per plant, 195 g of ash and 120 g of castor bean cake per plant, 230 g of ash and 140 g of castor bean cake per plant; 265 g of ash and 160 g of castor bean cake per plant, and 300 g of ash and 180 g of castor bean cake per plant) productivity of tomato cultivation. In 2014, in a factorial scheme (3 x 2), evaluated the effect of three wood ash levels (40, 80 and 120 g per plant) and two castor bean cake doses (140 and 280 g per plant) growth and productivity of the tomato crop. In 2015 we evaluated the effect of four irrigation levels (135, 165, 191 and 213 mm) on the growth and productivity of the tomato crop. The results in 2013 showed that the ash doses tested and castor bean cake did not influence the marketable productivity of tomato (1.24 to 1.59 kg per plant (24.8 to 31.8 t ha-1)). In 2014 most of the growth variables were positively influenced by the increase in wood ash and castor bean cake doses. Most marketable yield was obtained with the dose of 280 g of castor bean cake (1.99 kg per plant (39.8 t ha-1)), not significantly influenced by the ash doses. In 2015 most of the growth variables and marketable productivity were negatively with decreasing water depth. The level of 213 mm was responsible for greater marketable productivity (4.04 kg per plant (80.8 t ha-1)). / O presente trabalho intitulado ?Cultivo org?nico de tomate em vasoponia e ambiente protegido com manejo da irriga??o por acionador autom?tico? teve por objetivo proporcionar informa??es que sirvam de recomenda??o para um novo sistema de cultivo de tomate em vaso e em ambiente protegido, com rela??o aos insumos ?gua e fertilizante, visando uma produ??o sustent?vel, baseada principalmente na utiliza??o de materiais residu?rios como fonte de nutrientes e irriga??o automatizada de baixo custo. Os objetivos espec?ficos deste trabalho foram avaliar: (a) o efeito de diferentes doses de cinza e torta de mamona no crescimento e na produtividade da cultura do tomate; e (b) o efeito de diferentes l?minas de irriga??o no crescimento e na produtividade da cultura do tomate. Foram realizados tr?s experimentos no per?odo de 2013 a 2015 utilizando o delineamento experimental em blocos casualizados. Em 2013, avaliou-se o efeito de cinco doses crescentes de cinza e torta de mamona (160 g de cinza e 90 g de torta de mamona por planta; 195 g de cinza e 120 g de torta de mamona por planta; 230 g de cinza e 140 g de torta de mamona por planta; 265 g de cinza e 160 g de torta de mamona por planta; e 300 g de cinza e 180 g de torta de mamona por planta) na produtividade da cultura do tomate. Em 2014, em esquema fatorial (3 x 2), avaliou-se o efeito de tr?s doses de cinza (40, 80 e 120 g por planta) e duas doses de torta de mamona (140 e 280 g por planta) no crescimento e na produtividade da cultura do tomate. Em 2015 avaliou-se o efeito de quatro l?minas de irriga??o (135, 165, 191 e 213 mm) no crescimento e na produtividade da cultura do tomate. Os resultados obtidos em 2013 mostraram que as doses de cinza e torta de mamona n?o influenciaram significativamente a produtividade comercial do tomateiro (1,24 a 1,59 kg por planta (24,8 a 31,8 t ha-1)). Em 2014 a maioria das vari?veis de crescimento avaliadas foram influenciadas positivamente pelo aumento das doses de cinza e torta de mamona. A maior produtividade comercial foi obtida com a dose de 280 g de torta de mamona (1,99 kg por planta (39,8 t ha-1)), n?o sendo influenciada significativamente pelas doses de cinza. Em 2015 a maioria das vari?veis de crescimento e a produtividade comercial foram influenciadas negativamente com a diminui??o da l?mina de irriga??o. A l?mina de 213 mm foi a respons?vel pela maior produtividade comercial (4,04 kg por planta (80,8 t ha-1)).
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