This paper is about analyzing water quality of a stream that flows in southwest of Swedencalled Hasslebäcken. The stream is a tributary of Suseån and has its springs in the naturereserve Biskopstorp and flows through a mixed landscape of mostly spruce forests andagriculture land. Little is known about Hasslebäcken and its water and therefore a study by request of Suseåns vattenråd was performed. The study consisted of analyzing the water ofphosphorous, nitrogen, pH, suspended particles and conductivity. To broaden the examination of water quality sampling of benthic macroinvertebrates and two index (ASPTindex and Shannon’s diversity index) of water quality were used. Another part of the study were to search for fish barrier in the stream and to evaluate the possibilities for troutspawning and habitats. The whole stretch of the stream were examined and eleven differentsampling sites were used. The results show that Hasslebäckens upper parts have low levelsof nutrients while the lower parts show intermediate to extremely high levels. The indexresults points at low ecological values for Shannon and high ecological values for ASPT. Thisis normal for a stream that flows through an agricultural landscape. The results concerning pH show a lower pH in the upper parts than in the lower parts. This is also normal for a stream in a production forest of spruce in these parts of Sweden. Hasslebäcken also have several fish barrier that prevent various fish species to migrate up along the stream from thesea. The evaluation of trout habitats and spawning area show decent possibilities for successful reproduction and growth.