Animals performing on simple fixed-ratio (FR) schedules vi typically pause after reinforcement delivery. The present study demonstrated systematic control of pause length in multiple fixed ratio schedules by manipulating FR size and reinforcement magnitude. In Experiment I, two adult male rabbits were stabilized on an alternating, two component, multiple FR 10 FR 10 schedule. Different colored lights were correlated with both FR components. Stability was determined and mean pause lengths were calculated as one FR component was increased in size until the schedule was FR 10 FR 50. Pausing was longer before the large FR component and was positively correlated with increases in FR size. Schedule and stimulus control were demonstrated by randomizing the order of FR presentations and by reversing the colored lights previously correlated with either the small or large FR components. Existing pause differentials were not disrupted under the light reversal or random conditions. In Experiment II, the same rabbits were stabilized at mult FR 10 FR 30 with 1/2 cc of water delivered after each component. In subsequent conditions the magnitude of reinforcement (cc's of water) delivered at the completion of the large FR component, was systematically shifted from 4 cc, to 3 cc, 2 cc, 1 cc and finally back to 1/2 cc. Pausing before the large FR was found to be inversely related to the magnitude of reinforcement delivered in that component. Clearly the animals in this experiment discriminated upcoming schedule conditions and paused relative to FR size and reinforcement magnitude. Hence, it is proper to point out that the term "post-reinforcement pause" is a misnomer since it incorrectly implies a functional relation between pause length and prior schedule conditions.

The present study was designed to investigate the effects of a stimulus change inserted in the large component of a mixed fixed-ratio 10 fixed-ratio 100 schedule. In mixed fixed-ratio schedules, a run of responses which approximates the response requirement of the smaller fixed ratio occurs at the beginning of the larger fixed ratio. This run of responses and the pause it precedes is called a prime. In Experiment I, priming acquisition was compared in a mixed schedule in which a change in key-color followed completion of the first 10 responses of the fixed-ratio 100 component and a mixed schedule with no stimulus change. Primes were acquired more rapidly in the mixed schedule with the stimulus change and the number of responses in a priming run was less variable than occurred without the stimulus change. In Experiment II, the effect of the stimulus change on primes was further investigated by removal of the smaller fixed-ratio component or by varying the location of the stimulus change. Primes occurred only when the smaller fixed-ratio component was present. Varying the location of the stimulus change resulted in the transfer of control from the external stimulus to response-produced stimuli. Future investigation of this point of transfer should prove useful in the study of the proprioceptive stimulus control of homogeneous behavior sequences. In Experiments Ill through VI, the variables controlling the length of the post-prime and the post-reinforcement pauses were investigated using the mixed FR x chained FR x FR y schedule. In this series of experiments, FR x was varied with FR y held constant at both high and low values. In addition, FR y was varied with FR x held constant at both high and low values. The results indicate that the post-prime pause is primarily a function of FR y, the number of responses required after the priming run. On the other hand, both post-reinforcement pauses were shown to be a function of FR x, the size of the small fixed ratio. An interaction between FR x and FR y and both the post-reinforcement and the post-prime pauses suggested that pausing in mixed schedules is a closed system. Taken as a whole, these results indicate the importance of mixed chained schedules in the investigation of the priming phenomenon.

Four pigeons had discrimination training that required the choice of a left side-key following completion of a fixed-ratio 10 an the center key, and a right side-key response after fixed-ratio 20. Correct choices were reinforced on various fixed-interval, fixed-ratio, random-interval, and random-ratio schedules. When accuracy was examined across quarters of intervals (fixed-interval schedules) or quarters of median interreinforcerrent intervals (fixed-ratio schedules), accuracy was usually laver in the second quarter than in the first, third, or fourth quarters. When accuracy was examined across quarters of ratios (fixed-ratio schedules) or quarters of median number of correct interreinforcement trials (fixed-interval schedules), accuracy increased across quarters. These accuracy patterns did not occur m random-interval or random-ratio schedules. The results indicate that, when choice patterns differed on fixed-interval and fixed-ratio schedules, these differences were due to the methods of data analyses.

For contacting a virtual cylinder located near the centre of the experimental tank, 4 male Siamese fighting fish (Betta splendens) received food reinforcement on a fixed-ratio (FR) 6 schedule. Ten times per second a tracking system recorded, in 3 dimensions, the location of the fish in the experimental tank. For each fish, the number of responses per session, the 2 dimensional swimming patterns, and the response patterns as observed in cumulative response plots were analyzed in 4 experimental conditions. The experimental conditions were: (a) both a response-location stimulus and a response-feedback stimulus were present; (b) neither a response-location nor a response-feedback stimulus were present; (c) only a response-location stimulus was present; (d) only a response-feedback stimulus was present. After experiencing the conditions, 2 fish were exposed to extinction, first with only the response-location stimulus present and then with the addition of the response-feedback stimulus. The combination of the response-location and the response-feedback stimulus and the response-location stimulus alone resulted in the highest number of responses per session and swimming patterns more concentrated in and around the target response location. The absence of a response-location stimulus and a response-feedback stimulus resulted in fewer responses per session and swimming patterns more spread out than in the conditions where the response-location stimulus was present. The response-feedback stimulus alone produced patterns similar to the condition where neither stimuli were present. In neither condition was an FR break-and-run response pattern observed in the cumulative response plots. The addition of the feedback-stimulus to the extinction resulted in a temporary increase in responding for one fish, but not for the other. The results suggested that the response-location stimulus functioned as a discriminative stimulus and that the response-feedback stimulus functioned as a conditioned reinforcer when combined with the coin, though its role in the absence of the coin was less clear. The characteristics of the response topography and the variability in the location of the consumption response may have contributed to the lack of a break-and-run pattern under FR6 with fish.

Various methods were tried in an attempt to obtain a technique for quantifying the gnawing effects of apomorphine on rats. A technique using a restraining tube was developed. Under a 2 milligram per kilogram intraperitoneal injection of apomorphine, four female Long Evans hooded rats were placed on continuous and fixed reinforcement schedules using a gnawable pine block. Subjects would learn to turn their heads away from the gnawable object in order to obtain 15 seconds of gnawing time. The rate of response increased from less than one response in 5 minutes to over 3 responses per minute in 10 one-half hour conditioning sessions. Rates of response stabilized during the last 5 experimental sessions and fixed ratio schedules of up to 5:1 were obtained in five additional 1 '1/ 2 hour sessions.

Rationale: Amphetamine abuse by adolescents predicts long-term drug dependence. Heightened vulnerability to drug abuse could be due to higher sensitivity to drug’s reinforcing effects. Rodents are used to study age-related sensitivities to drugs. Objective: We compared intravenous amphetamine self-administration between adolescent and adult male rats on an operant schedule of reinforcement measuring the reinforcing efficacy of a drug. Methods: After surgery, adolescent and adult rats acquired lever-pressing behavior reinforced by amphetamine infusions. Results: Both age groups exhibited more infusions per session as dose increased. However, neither the number of infusions per session nor total amphetamine intake differed across age groups. Conclusion: Although rapid transition is reliable to test reinforcing properties of stimulants, results suggest that amphetamine is an equally efficacious reinforcer among both age groups. In regards to humans, these results suggest that other factors, like social influences, explain higher rates of drug intake by adolescent compared with adult humans.

Most studies of gustatory coding have been performed in either anesthetized or awake, passively stimulated rats. In this dissertation the influences of behavioral state on gustatory processing in awake rats are described. In the first set of experiments, the effects of non-contingent tastant delivery on the chemical tuning of single neurons were explored. Tastants were delivered non-contingently through intra-oral cannulas to restrained, non water-deprived rats while single unit responses were recorded from the gustatory cortex (GC). As the subjects' behavior progressed from acceptance to rejection of the tastants, the chemical tuning of the neurons changed as well. This suggests that the subjects' behavioral state powerfully influences gustatory processing. In the second set of experiments, rats were trained to lick for fluid reinforcement on an FR5 schedule while single unit activity was recorded from GC. In this case, the chemical tuning was much more stable. Under this paradigm, chemosensory responses were rapid (~ 150 ms) and broadly tuned. In the third study, it was found that ensembles of GC neurons could discriminate between tastants and their concentrations on a single trial basis, and such discrimination was accomplished with a combination of rate and temporal coding. Ensembles of GC neurons also anticipated the identity of the upcoming stimulus when the tastant delivery was predictable. Finally, it was found that ensembles of GC neurons could discriminate between the bitter stimuli nicotine and quinine. Nicotine is both a bitter tastant and a trigeminal stimulant, and when the acetylcholine receptors in the lingual epithelium were blocked with mecamylamine, the ensembles failed to discriminate nicotine from quinine.

Propofol is a widely in use anesthetic drug. Propofol’s abuse liability has been supported by many case reports and a few animal studies. However, propofol’s reinforcing properties have not yet been investigated in-depth. In this study, multiple aspects of propofol’s abuse-related behaviour were investigated using the drug self-administration model in rats. METHODS: Rats were subjected to propofol self-administration under a fixed ratio 1 (FR1) schedule and different aspects of propofol self-administration behaviour including acquisition, maintenance of the behaviour under a higher ratio schedule, extinction and reinstatement were investigated. RESULTS: Rats acquired propofol self-administration under a FR1 schedule. The acquired behaviour was maintained under a FR2 schedule, showed a modest variation over a range of doses, and was extinguished upon substitution of vehicle for propofol, showing no reinstatement using a range of priming doses of propofol. CONCLUSION: Propofol has abuse potential showing modest reinforcing properties under our experimental conditions.

Propofol is a widely in use anesthetic drug. Propofol’s abuse liability has been supported by many case reports and a few animal studies. However, propofol’s reinforcing properties have not yet been investigated in-depth. In this study, multiple aspects of propofol’s abuse-related behaviour were investigated using the drug self-administration model in rats. METHODS: Rats were subjected to propofol self-administration under a fixed ratio 1 (FR1) schedule and different aspects of propofol self-administration behaviour including acquisition, maintenance of the behaviour under a higher ratio schedule, extinction and reinstatement were investigated. RESULTS: Rats acquired propofol self-administration under a FR1 schedule. The acquired behaviour was maintained under a FR2 schedule, showed a modest variation over a range of doses, and was extinguished upon substitution of vehicle for propofol, showing no reinstatement using a range of priming doses of propofol. CONCLUSION: Propofol has abuse potential showing modest reinforcing properties under our experimental conditions.

The present study contributes an apparatus and research paradigm useful in generating human performances sensitive to concurrent schedules of reinforcement. Five participants produced performances observed to be under temporal and ratio control of concurrent fixed interval-fixed ratio schedules. Two aspects of interaction between FI and FR schedules were distinguishable in the data. First, interaction between two schedules was observed in that changes in the value of one schedule affected behavior reinforced on another schedule. Second, switching from one pattern to the other functioned as an operant unit, showing stability during schedule maintenance conditions and sensitivity to extinction. These effects are discussed in the context of current views on behavior under concurrent schedules of reinforcement, and some implications for the conceptualization, measurement, analysis, and treatment of complex behavior are presented.