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Showing 1 to 2 of 2 (0.073 seconds)Spelling suggestions: "subject:"fledgling"" "subject:"fledging""
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Postfledging Survival and Movements of Willow and Dusky Flycatchers in the Central Sierra NevadaVormwald, Lisa M. 2010 August 1900 (has links)
Understanding factors limiting population growth is critical for species exhibiting declining populations. Reproductive success has an important effect on population dynamics; however, our ability to accurately estimate productivity is limited. Studies on avian breeding biology have focused on nest survival; however, surviving to fledging does not ensure survival to the end of the breeding season. Furthermore, our understanding of habitat selection by birds based on the nesting cycle may not adequately represent the breeding habitat requirements because habitat use often changes after the young leave the nest. My goal was to examine the postfledging dependence period of two flycatcher species in the central Sierra Nevada: the California state endangered willow flycatcher (Empidonax traillii) and the dusky flycatcher (E. oberholseri). My focus was to estimate fledgling survival and examine factors that influence survival, evaluate postfledging movements and habitat use, and estimate post-breeding home range sizes of postfledging flycatchers. I monitored nests of both flycatcher species, individually color banded nestlings, and observed family groups daily once the young fledged. Flycatcher fledgling survival ranged from 46 percent to 76 percent and varied by year and species. Survival was lowest during the first week of the postfledging dependence period for both species. Fledgling flycatchers moved on average ~45m per day during the dependence period. I detected family groups in the natal meadows from 13 to 33 days. I detected willow flycatchers in riparian shrub vegetation 94 percent of the time, with the remaining detections being along the upland forest edge. Dusky flycatchers were more likely to use upland forest vegetation after leaving the nest, as I detected them in riparian shrub vegetation 70 percent of the time. For both years combined, mean 95 percwnt home range sizes were 1.80 ± 1.44 ha for willow flycatchers and 1.82 ± 1.70 ha for dusky flycatchers. Mean 50 percent core areas were 0.33 ± 0.27 ha for willow flycatchers and 0.38 ± 0.44 ha for dusky flycatchers. My results suggest that using fledgling survival throughout the dependence period to assess reproductive output is more accurate than using nesting data alone. Furthermore, postfledging family groups used a larger area of habitat than what is typically estimated from territory mapping singing males. Future research should continue to stress the importance of gaining knowledge about the postfledging period, especially for species with declining populations.
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Development of Novel High-Resolution Melting (HRM) Assays for Gender Identification of Caribbean Flamingo (Phoenicopterus ruber ruber) and other BirdsChapman, Alexandra 14 March 2013 (has links)
Unambiguous gender identification (ID) is needed to assess parameters in studies of population dynamics, behavior, and evolutionary biology of Caribbean Flamingo (Phoenicopterus ruber ruber) and other birds. Due to its importance for management and conservation, molecular (DNA-based) avian gender ID assays targeting intron-size differences of the Chromosome Helicase ATPase DNA Binding (CHD) gene of males (CHD-Z) and females (CHD-W) have been developed. Male (ZZ) and female (WZ) genotypes are usually scored as size polymorphisms through agarose or acrylamide gels. For certain species, W-specific restriction sites or multiplex polymerase chain-reaction (PCR) involving CHD-W specific primers are needed.
These approaches involve a minimum of three steps following DNA isolation: PCR, gel electrophoresis, and photo-documentation, which limit high throughput scoring and automation potential. In here, a short amplicon (SA) High-resolution Melting Analysis (HRMA) assay for avian gender ID is developed. SA-HRMA of an 81-Base Pair (bp) segment differentiates heteroduplex female (WZ) from homoduplex male (ZZ) genotypes by targeting Single-nucleotide Polymorphisms (SNPs) instead of intron-size differences between CHD-Z and CHD-W genes.
To demonstrate the utility of the approach, the gender of Caribbean Flamingo (P. ruber ruber) (17 captive from the Dallas Zoo and 359 wild from Ria Lagartos, Yucatan, Mexico) was determined. The assay was also tested on specimens of Lesser Flamingo (P. minor), Chilean Flamingo (P. chilensis), Saddle-billed Stork (Ephippiorhynchus senegalensis), Scarlet Ibis (Eudocimus ruber), White-bellied Stork (Ciconia abdimii), Roseate Spoonbill (Platalea ajaja), Marabou Stork (Leptoptilos crumeniferus), Greater Roadrunner (Geococcyx californianus), and Attwater's Prairie Chicken (Tympanuchus cupido attwateri). Although the orthologous 81 bp segments of Z and W are highly conserved, sequence alignments with 50 avian species across 15 families revealed mismatches affecting one or more nucleotides within the SA-HRMA forward or reverse primers. Most mismatches were located along the CHD-Z gene that may generate heteroduplex curves and thus gender ID errors. For such cases, taxon and species-specific primer sets were designed. The SA-HRMA gender ID assay can be used in studies of avian ecology and behavior, to assess sex-associated demographics and migratory patterns, and as a proxy to determine the health of the flock and the degree by which conservation and captive breeding programs are functioning.
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