In the present study, a two-dimensional numerical model based on a time-independent finite difference method was developed and the model is used to analysis the dynamic interaction among wave, sloshing fluid in tank and the floating tank. The free surface of wave and sloshing fluid in the tank are all assumed to be a single value function and the wave breaking is, therefore, not considered in the study. The numerical model is firstly validated by some bench make studies. Extensive simulation were made to discuss the effects of geometry of the floating tank, the ratio of depth to breadth of fluid in tank, the fundamental freq of floating tank ¡V structure system etc.

Secondary salinisation has rendered over 100 million hectares of land throughout the world, and over 5 million hectares in Australia, unsuitable for conventional agriculture. The utilization of salinised land and its associated water resources for mariculture is an adaptive approach to this environmental problem with many potential economic, social and environmental benefits. Despite this, inland mariculture is yet to develop into an industrial-scale, rural enterprise. The main aim of this study was therefore to identify and address some of the technical and biological limitations to the development of an inland finfish mariculture industry. Three technical aspects essential to the development of an Australian inland mariculture industry were reviewed; potential sources of water, the species suitable for culture in these water sources and the production systems available to produce them. Based on factors such as their quantity, quality and proximity to infrastructure, the most appropriate water sources were deemed to be groundwater obtained from interception schemes and waters from operational or disused mines. In terms of species, mulloway (Argyrosomus japonicus) were identified as having many positive attributes for inland mariculture, including being temperate and therefore having the ability to be cultured year-round in the regions where the majority of secondary salinity occurs. Seasonal production of barramundi (Lates calcarifer) in ponds in the temperate climatic zones has potential, but may be more appropriate for those salinised water sources located in the warmer parts of the country. Rainbow trout (Oncorhynchus mykiss) were also identified as having excellent potential provided water temperature can be maintained below the upper lethal limit and also have potential for seasonal production, perhaps in rotation with barramundi. In terms of production systems, pond-based culture methods were found to have many advantages specific to inland mariculture. Static ponds enable culture in areas with low groundwater yield and more cost-effective potassium supplementation compared with flow through ponds. Static ponds also largely overcome the issues associated with the disposal of salt-laden and eutrophied waste water; however yields from static ponds are typically low and limited by the nutrient input into the pond. In response to the yield constraints of static pond culture, a new culture technology known as the Semi-Intensive Floating Tank System (SIFTS) was designed, patented and constructed in collaboration with the aquaculture industry and tested in a static inland saline pond in the wheatbelt of Western Australia. This technology was designed to reduce nutrient input into ponds by the collection of settleable wastes and to provide large volumes of well-oxygenated water to the target species, to ameliorate the loss of fish from low dissolved oxygen during strong microalgal blooms. The three species identified above has having excellent potential for inland mariculture (mulloway, rainbow trout, and barramundi) were grown in SIFTS held within a 0.13 ha static, inland saline water body (salinity 14 ppt) over a period of 292 days, yielding the equivalent of 26 tonnes/ha/year (total for all three species). Rainbow trout were grown with an FCR of 0.97 from 83 to 697 grams over 111 days (SGR, 1.91%/day) between June and September, when average daily water temperatures ranged from 12.3„aC to 18.2„aC. Over the same time period, mulloway grew only from 100 to 116 grams, however, once temperatures increased to approximately 21„aC in October, feed intake increased and mulloway grew to an average size of 384 grams over 174 days with an SGR and FCR of 0.68 %/day and 1.39, respectively. Barramundi stocked in November with an average weight of 40 grams increased to 435 grams in 138 days (SGR 1.73%/day) with an FCR of 0.90. The SIFTS significantly reduced nutrient input into the pond by removing settleable wastes as a thick sludge with a dry matter content of 5 to 10%. The total quantity of dry waste removed over the 292 day culture period was 527 kg (5 tonnes/ha/yr), which was calculated to contain 15 kg of nitrogen (144 kg/ha/yr) and 16 kg of phosphorus (153 kg/ha/yr). The release of soluble nutrients into the pond resulted in blooms of macro- and micro- algae which caused large and potentially lethal diurnal fluctuations in dissolved oxygen within the pond, however, comparatively stable levels of dissolved oxygen were maintained within each SIFT through the use of air lift pumps. It is well documented that saline groundwater is deficient in potassium which, depending on the extent of the deficiency, can negatively impact on the performance of marine species, including fish. The physiological effects of this deficiency on fish, however, have not been previously described. As such, I conducted a bioassay investigating the physiological effects of a hypersaline (45 ppt) groundwater source containing 25% of the potassium found in equivalent salinity seawater (i.e. 25% K-equivalence) on juvenile barramundi. Histopathological examination of moribund fish revealed severe degeneration and necrosis of skeletal muscles, marked hyperplasia of branchial chloride cells and renal tubular necrosis. Clinical chemistry findings included hypernatraemia and hyperchloridaemia of the blood plasma and lowered muscle potassium levels. It was concluded from this study that the principal cause of death of these barramundi was skeletal myopathy induced by unsustainable buffering of blood plasma potassium levels from the muscle. Although such hypokalaemic muscle myopathies have been previously described in mammals and birds, this was the first description of such myopathies in fish. It was hypothesized from the results described above that the physiological effects of potassium deficiency are dependent on salinity and that they would be ameliorated by potassium supplementation. These predictions were tested in a subsequent study which measured the effects of potassium supplementation between 25% and 100% K-equivalence on the growth, survival and physiological response of juvenile barramundi at hyperosmotic (45 ppt), near-isosmotic (15 ppt) and hyposmotic (5 ppt) salinities. Unlike those juvenile barramundi reared at 45 ppt and 25% K-equivalence in the previous study, those reared in 50% K-equivalence water at 45 ppt in this study survived for four weeks but lost weight; whereas at 75% and 100% K-equivalences fish both survived and gained weight. Homeostasis of blood plasma potassium was maintained by buffering from skeletal muscle. Fish reared in 50% K-equivalence at this salinity exhibited muscle dehydration, increased branchial, renal and intestinal (Na+-K+)ATPase activity and elevated blood sodium and chloride, suggesting they were experiencing osmotic stress. At 15 ppt, equal rates of growth were obtained between all K-equivalence treatments. Buffering of plasma potassium by muscle also occurred but appeared to be in a state of equilibrium. Barramundi at 5 ppt displayed equal growth among treatments. At this salinity, buffering of plasma potassium from muscle did not occur and at 25% K-equivalence blood potassium was significantly lower than at all other K-equivalence treatments but with no apparent effect on growth, survival or (Na+-K+)ATPase activities. These data confirmed the hypothesis that proportionally more potassium is required at hyperosmotic salinities compared to iso- and hypo- osmotic salinities and also demonstrated that barramundi have a lower requirement for potassium than other marine and estuarine species being investigated for culture in inland saline groundwater. In addition to ongrowing fish, saline groundwater has potential for hatchery production. Specific advantages include the vertical integration of inland saline farms and the production of disease-free certified stock through isolation from the pathogens and parasites found naturally in coastal water. To determine the potential of utilizing inland saline groundwater for hatchery production, barramundi larvae were reared from 2 to 25 days post hatch in 14 ppt saline groundwater with either no potassium supplementation (38% K-equivalence) or full potassium supplementation (100% K-equivalence). Growth, survival and swimbladder inflation of these larvae were compared against those grown in control treatments of seawater (32 ppt) and seawater diluted to 14 ppt. Those reared in saline groundwater with 38% K-equivalence exhibited complete mortality within 2 days, whilst those held in groundwater with full supplementation survived at a rate equal to both control treatments (pooled average 51.1 ¡Ó 0.5%). At 25 days post hatch, there was no significant difference in larval length or dry weight between those grown in the 14 ppt control treatment and those in the saline groundwater with full potassium supplementation. There were no significant differences in swim bladder inflation between any of the surviving treatments (average 93.3 ¡Ó 2.5%). This is the first description of rearing barramundi larvae both in low salinity seawater and in saline groundwater, and demonstrates that the requirement for potassium by larval barramundi is higher than for juveniles of the same species. In addition to a deficiency in potassium, saline groundwater in Western Australia often contains an elevated concentration of manganese relative to seawater as a result of anaerobic reduction of manganese oxides or the pedogenic weathering of manganese-bearing rock. The effects of elevated manganese on marine or estuarine fish have not been described and a study was therefore conducted to determine if manganese, at a concentration typical of that found in saline groundwater, has any impact on fish. The effects of 5 mg/L of dissolved manganese on juvenile mulloway at salinities of 5, 15 and 45 ppt were determined by comparing the survival, growth and blood and organ chemistry with those grown at the same salinities without manganese addition. Survival of mulloway at 45 ppt in the presence of 5 mg/L of manganese (73 ¡Ó 13%) was significantly lower than all other treatments, which achieved 100% survival. Those fish grown in seawater without manganese exhibited rapid growth, which was not affected by salinity (SGR = 4.05 ¡Ó 0.29%/day). Those fish grown at 5 ppt and 45 ppt in the presence of manganese lost weight over the two week trial (SGR 0.17 ¡Ó 0.42 and -0.44 ¡Ó 0.83%/day, respectively), whilst those at 15 ppt gained only a small amount of weight (SGR 1.70 ¡Ó 0.20%/day). Growth was therefore affected by manganese and by the interaction of manganese and salinity, but not salinity alone. Manganese was found to accumulate in the gills, liver and muscle of the fish. No gill epithelial damage or other significant histological findings were found, however, significant differences in blood chemistry were observed. Blood sodium and chloride of manganese exposed fish were significantly elevated in hyperosmotic salinity (45 ppt) and depressed at hyposmotic salinity (5 ppt) compared with unexposed fish; consistent with manganese causing apoptosis or necrosis to chloride cells. Blood potassium was significantly elevated and liver potassium significantly reduced at all salinities in the presence of manganese. These findings are consistent with manganese interfering with carbohydrate metabolism. There were no differences in blood sodium, chloride or potassium across salinities in fish not exposed to manganese, demonstrating mulloway are capable of efficient osmoregulation across this salinity range.

Thesis (MScAgric (Agricultural Economics))--University of Stellenbosch, 2011. / ENGLISH ABSTRACT: The rising demand of fish due to population growth coupled by stagnation of fish supply from natural capture has led the world to turn to aquaculture to fill in the gap between fish supply and demand. Aquaculture has emerged as the only sustainable way of supplying the rising population with fish. However the rapid expansion of aquaculture has been met with growing concerns over its environmental effects especially waste produced from aquaculture. The net cage system that is currently being used by small scale trout farmers in the Western Cape is an open water based system where release of waste into the water bodies is inevitable and this put into question the long term sustainability of trout farming using net cages in irrigation dams in the Western Cape. This study sought to compare identified production techniques that can be used by aquaculture farmers to reduce accumulation of organic waste in irrigation dams. The proposed ‘clean’ production techniques include use of net cages fitted with Lift-up system, semi intensive floating tank system (SIFTS) and intergrated aquaculture systems. The study revealed that the intergrated aquaculture system is the most effective way of recovering waste that shows great potential of moving aquaculture towards long term sustainability as it fullfills sustainability dimensions such as ‘zero emission’, nutrient recycling and integrated production. Mechanical methods of recovering waste such as Lift-up system and SIFTS are also effective in recovering particulate waste but however dissolved nutrients are lost into the environment. The study went on further to investigate if economic, environmental and social benefits of recovering waste from irrigation dams outweigh the costs of recovering waste using different production techniques. Models of small scale aquaculture farms using the three identified production techniques were developed and compared with a modelled small scale net cage farm where there was no waste recovery. A comparative financial analysis of the modelled small scale trout farms using alternative production techniques carried out showed that trout production using any of the three alternative ‘clean’ production techniques is financially viable with the SIFTS production technique giving the farmer the highest returns, followed by the intergrated system, then the net cage with a Lift-up system and lastly the net cage system without waste recovery. The second part of the study used the contingent valuation method to estimate the environmental and social benefits of removing waste from dams. Households revealed that they were willing to pay (WTP) R40 on average annually to improve water quality from a state where eutrophication had occurred to a state suitable for irrigation and aquaculture. To improve water quality from a state suitable for irrigation to a state suitable for swimming, households were willing to pay R16.67 annually. If water was to be improved from a state suitable for irrigation to a level suitable for domestic purposes, average willingness to pay (WTP) was R26.17 annually. WTP indicate that besides financial benefits associated with using ‘clean’ production techniques there are environmental and social benefits that will arise to the farm community using water from the irrigation dams. / AFRIKAANSE OPSOMMING: Die stygende vraag na vis as gevolg van bevolkingsgroei, tesame met die stagnering van die aanbod van vis vanaf natuurlike vangste het daartoe aanleiding gegee dat die oë van die wêreld op akwakultuur gerig is om die gaping in die voorsiening van vis te vul. Akwakultuur het ontwikkel as die enigste volhoubare manier om aan die groeiende vraag na vis te voldoen. Die vinnige uitbreiding van akwakultuur het egter toenemende besorgdheid in die nadelige omgewingsimpak, veral ten opsigte van akwakultuurafval, tot gevolg gehad. Die nethokstelsel wat tans deur kleinskaalse forelboere in die Wes-Kaap in oop watergebaseerde sisteme gebruik word en die vrystelling van afval in die wateromgewings wat onafwendbaar is, plaas ’n vraagteken oor die langtermyn volhoubaarheid van die nethokstelsel forelboerdery in besproeiingsdamme in die Wes- Kaap. Die studie het ten doel gehad om geïdentifiseerde produksiestelsels wat deur akwakultuurboere gebruik kan word om die akkummulasie van organiese afval in besproeiingsdamme te verminder, te vergelyk. Die voorgestelde “skoon” produksietegnieke sluit in nethokke wat aan ’n opligstelsel gekoppel word, ‘n semi-intensiewe drywende tenk- stelsel (“SIFTS system” in Engels) en ‘n geïntegreerde akwakultuurstelsel. Met hierdie studie is bevind dat die geïntegreerde stelsel die mees effektiewe manier is om afval te herwin en toon potensiaal om akwakultuur op ’n vohoubare pad te plaas aangesien dit aan die volhoubaarheidsdimensies van geen emissie, voedingstofherwinning en geïntegreerde produksie voldoen. Meganiese metodes van afvalherwinning soos die nethokopligstelsel en die SIFTS-stelsel is effektief in die herwinning van vastestofdeeltjies, maar opgeloste voedingstowwe word steeds in die omgewing vrygestel. Die studie het voorts ten doel gehad om te bepaal of die ekonomiese, omgewings- en sosiale voordele om afval uit besproeiingsdamme te herwin, groter is as die herwinningskoste van die verskillende produksietegnieke. Modelle van kleinskaalse akwakultuurplase wat die drie geïdentifiseerde produksiestelsels gebruik, is ontwikkel en aangewend om te vergelyk met ’n nethokstelsel waar geen afvalherwinning gedoen word nie. ’n Vergelykende finansiële ontleding van die gemodelleerde kleinskaalse forelboerderye met die verskillende produksietegnieke is gedoen en daar is bevind dat enige een van die drie “skoon” stelsels finansieel lewensvatbaar is, met die SIFTS-stelsel wat die hoogste vergoeding aan die boer bied, gevolg deur die geïntegreerde stelsel, dan die nethokke aan ’n opligstelsel en dan die nethokstelsel sonder afvalherwinning. Die tweede deel van die studie het van die voorwaardelike (“contingent”) waardasiemetode gebruik gemaak om die omgewings- en sosiale voordele om afval uit besproeiingsdamme te verwyder, te bepaal. Huishoudings het aangetoon dat hulle bereid sou wees om tot R40 per jaar te betaal om die waterkwaliteit te verbeter vanaf ’n toestand waar eutrifikasie plaasgevind het na ’n toestand waar die water vir besproeiing en akwakultuur geskik sou wees. Om die waterkwaliteit vanaf ’n toestand geskik vir besproeiing te verander na ’n toestand geskik om in te swem, sou huishoudings bereid wees om R16.67 per jaar te betaal. Indien water vanaf ’n toestand geskik vir besproeiing verander sou word na ’n toestand geskik vir huishoudelike gebruik, sou huishoudings gewillig wees om jaarliks R26.17 te betaal. Die “gewilligheid om te betaal” dui aan dat daar bo en behalwe die finansiële voordele om van “skoon” produksietegnieke gebruik te maak, ook omgewings- en sosiale voordele vir die plaasgemeenskap bestaan met die gebruik van die water uit die besproeiingsdamme.