Forests provide important ecosystem services and play a dominant role in the global carbon and hydrologic cycles. These ecosystems are becoming more vulnerable to climate change-related threats such as extreme temperature and precipitation events, drought and wildfires. In addition, forest ecosystems have also undergone land use changes and a significant reduction in cover area, specifically in North America. There has been renewed realization to restore and rehabilitate forest ecosystems because they are a major carbon sink and play a key role in sequestering atmospheric carbon dioxide. In response, plantation forests are being widely established to sequester carbon, increase biodiversity, secure water resources and generate economic revenue when harvested. Forest managers employ different management practices such as thinning or retention harvesting to enhance growth, plant structural and species diversity within forest plantations, with the ultimate goal of emulating the characteristics and benefits of natural forests. However, the influence of these forest management practices on the growth, productivity and specifically water cycling in plantation forests is not well studied and reported in the literature. This experimental study investigated the effect of four different variable retention harvesting (VRH) treatments on evapotranspiration and water balance in an 83-year-old red pine (Pinus resinosa) plantation forest in the Great Lakes region in Canada. These VRH treatments included 55% aggregated crown retention (55A), 55% dispersed crown retention (55D), 33% aggregated crown retention (33A), 33% dispersed crown retention (33D) and unharvested control (CN) plot. Tree-level experimental work was conducted in the control plot and showed that most of the water transport (65%) occurred in the outermost sapwood, while only 26% and 9% of water was transported in the middle and innermost depths of sapwood, respectively. These results help to avoid overestimation of transpiration, which may cause large uncertainties in water budgets in pine forests. Study results further showed that the 55D treatment had the highest tree-level transpiration followed by 33D, 55A, 33A and CN plots. During periods of low precipitation, vapor pressure deficit (VPD) was the main driver or control on transpiration in VRH treatments. However, transpiration was more closely coupled with photosynthetically active radiation (PAR) in the control plot. Moreover, the 55D treatment resulted in on average 58% of total water loss from canopy as transpiration and 42% from the understory and ground surface as evapotranspiration. These findings suggest that dispersed or distributed retention of 55% basal area (55D) provides the optimal environmental conditions for forest growth with reduced competition of trees for water as shown by enhanced transpiration. This study will help researchers, forest managers and decision-makers to improve their understanding of thinning impacts on water and carbon exchanges in forest ecosystems and select and adopt viable forest management practices to enhance their carbon sequestration capabilities, water use efficiency and resilience to climate change. / Thesis / Doctor of Philosophy (PhD)