Com o objetivo de compreender quais fatores abióticos determinam a ocorrência das diferentes formações florestais na região de Batatais, entre as coordenadas 20038S e 20058S e 47037W e 47028W, foram escolhidas três propriedades agrícolas (Fazenda Monte Belo, em Restinga, SP e Fazenda Magnólia e Sítio Pratinha, em Batatais, SP), que apresentavam remanescentes florestais representativos da região (floresta ribeirinha paludícula, floresta estacional semidecídua ribeirinha e cerradão). Para descrever a composição florística e a estrutura da comunidade arbórea foram amostradas 05 parcelas de 10 x 10 m ao redor de cada estação tensiométrica. Para o estudo dos fatores abióticos foram determinados os parâmetros físicos e químicos dos solos e as curvas de retenção de água nos solos e piezometria. A discriminação das variáveis abióticas e de vegetação foi feita através da análise de componentes principais (PCA) e, na correlação entre a abundância de espécies e fatores abióticos, foram feitas análises diretas de gradiente por meio de Análise de Correlação Canônica (CCA). A textura dos solos (teores de argila, silte e areia) e a posição no terreno (cota), foram as variáveis ambientais mais importantes na distribuição das espécies e nas variações das fisionomias florestais, com influência na retenção hídrica e nos índices de fertilidade dos solos. Essas diferenças nos teores de argila em profundidade ao longo do declive, com influência direta na retenção de umidade dos solos ou mesmo na formação de ambientes paludículos, determinaram grupos distintos de espécies e fisionomias florestais: (i) um grupo de espécies de ocorrência típica nas fisionomias de Cerrado e fortemente associado às cotas mais elevadas (solos menos férteis, bem drenados e porcentagens mais baixas de argila e silte nas camadas mais profundas), entre as quais: Matayba juglandifolia, Trichilia pallida, Lithraea molleoides, Anadenanthera falcata, Myrcia tomentosa, Copaifera langsdorffii, Erytrhoxylum pelleteranum, Platipodium elegans, Chrysophyllum marginatum, Luehea divaricata, entre outras e (ii) um grupo com espécies indicadoras de Florestas Paludículas ou de ocorrência comum nestas fisionomias, associado aos solos das cotas mais baixas (Gleissolos), com porcentagens altas de argila e silte nas camadas superficiais, mais férteis e maior retenção hídrica: Cecropia pachystachya, Citronella gongonha, Rapanea guianensis, Talauma ovata e Tapirira guianensis. Pode-se considerar um terceiro grupo de transição entre Floresta Estacional Semidecídua e Cerradão, nas cotas intermediárias e menos úmidas (espécies amostradas em parcelas localizadas nas bordas das Florestas Paludículas), onde ocorreram espécies tanto de ambiente de Cerradão, como de Floresta Estacional Semidecídua, e algumas poucas de ambiente paludículo. As porcentagens mais elevadas de Areia nos solos do Sítio Pratinha, com influência na menor retenção hídrica e também nos índices de fertilidade (solos álicos, com maior acidez e os menos férteis), podem explicar algumas diferenças na composição e abundância das espécies entre fisionomias florestais iguais. O Cerradão da Fazenda Monte Belo, mostrou-se diferente floristicamente do Cerradão do Sítio Pratinha. Esta mesma diferença ocorreu em relação às Florestas Paludículas da Fazenda Magnólia e a do Sítio Pratinha. As poucasespécies de ocorrência comum tiveram populações diferentes. A CCA evidenciou também correlações de algumas espécies com determinadas variáveis de solo. / In order to understand what abiotic factors determine the occurrence of different forest formations in the region of Batatais, coordinated between 20038\'S and 20058\'S and 47037\'W and 47028\'W, three agricultural properties were chosen (Monte Belo, in the city of Restinga, SP and Magnolia and Pratinha in Batatais, SP, Brazil), which had forests representative of the region (swamp forest, semi-deciduous riverine forest and forested savanna). To describe the floristic composition and structure of the arboreal community, 05 plots of 10 x 10 meters around each tensiometric station were sampled. For the study of abiotic factors, the physico-chemical characteristics of the soils, the soil-water-retention curves and the groundwater fluctuation were determined. The discrimination of the environmental variables and arboreal species were made through the Principal Components Analysis (PCA), and in the correlation between the abundance of species and abiotic factors, direct analyses of gradient through Canonical Correspondence Analysis (CCA) were made. The texture of the soil (levels of clay, silt and sand) and the position on the ground (altitude) were the most important environmental variables in the distribution of species and variations of forest physiognomies, with influence in the retention rates of water and soil fertility. These differences in levels of clay in depth along the slope, with direct influence on the retention of soil moisture and even in the development of swampy environment, determined distinct groups of species and forest physiognomies: (i) a group of species of typical occurrence in physiognomies of Forested Savanna and strongly associated with higher altitude (less fertile, well-drained soil and lower percentages of clay and silt in the deeper layers), including: Matayba juglandifolia, Trichilia pallida, Lithraea molleoides, Anadenanthera falcata, Myrcia tomentosa, Copaifera langsdorffii, Erytrhoxylum pelleteranum, Platipodium elegans, Chrysophyllum marginatum, Luehea divaricata, among others, and (ii) a group with indicator species of Swamp Forests or common occurrence in these physiognomies, associated with lower altitude (Gleisoil), with high percentages of clay and silt in the upper and more fertile layers, and greater water retention: Cecropia pachystachya, Citronella gongonha, Rapanea guianensis, Talauma ovata and Tapirira guianensis. A third group of transition between Semi-deciduous Forest and Forested Savanna, in intermediate altitude and soils less humid (species sampled in plots located at the edges of Swamp Forests), occurred both in the Forested Savanna and in Semi-deciduous Forest and a few in swampy environment. The highest percentages of sand in the soil of Pratinha, with less influence on water retention and also on the rates of fertility (alic soils, with greater acidity and less fertile), may explain some differences in the composition and abundance of species of forest physiognomies equal. The forested Savanna of Monte Belo, has proved floristically different from the Forested Savanna of Pratinha. This same difference occurred in swamp forests of Magnolia and Pratinha. The few species of common occurrence had differentpopulations. The CCA also revealed correlations of some species with certain variables of soil.

Dissolved organic matter (DOM) is present in all forested catchments and can be important in binding metals, absorbing UV, and the transport of nutrients (C, N, S, P). DOM is extremely heterogeneous in time and space, making it difficult to characterize. New techniques have been developed to determine δ34S and δ18O in DOM. These techniques have been applied to samples from Harp and Plastic Lake catchments (45º23'N, 79º 08'W, 45º11'N, 78º 50'W) in order to obtain information about sources and sinks of DOM within forested catchments on the Canadian Shield. In conjunction with sulphate and DOC concentrations, this new data provides valuable insight into sulphur cycling and DOM alteration within these catchments. Data generated for δ34S-DOM and δ18O-DOM appears to be the first data reported in the literature for DOM. The inorganic (δ34S-SO42-) and organic S (δ34S-DOM) differs by environment in both catchments. The range of δ34S-SO42- is between 3. 3‰ and 10. 3‰, and the range of δ34S-DOM is from 3. 4‰ to 8. 7‰. Sulphate in the Harp Lake catchment in most samples is subject to some sort of cycling within the watershed, since δ34S-SO42- differs from precipitation. In the Harp Lake catchment, upland δ34S-SO42- is influenced by historical precipitation. The δ34S-DOM is derived from leaching and microbial activity of DOM from organic horizons in the soil. The δ34S-SO42- and δ34S-DOM of wetland streams is extremely variable, controlled by hydrology. The δ34S-SO42- provides information on oxidation-reduction dynamics in the wetland, and δ34S-DOM provides information about sources of DOS in the wetland. The δ34S-SO42- and δ34S-DOM are possibly related in Harp Lake. Mineralization of DOS as evidenced by δ34S-DOM and DOS concentrations could be a small input of SO42- into Harp Lake. It is possible δ18O-DOM could be an indicator of DOM alteration. The range of δ18O-DOM is between 8. 2‰ and 14. 4‰. The δ18O-DOM in the Harp Lake catchment is highly correlated with relative molecular weight, which has been shown to decrease with increasing alteration. Wetland streams show the largest range in δ18O-DOM, while uplands, groundwater, and Harp Lake are the least varied. The highest δ18O-DOM values are from sources of DOM such as leaf leachates (representative of forest floor litter) and wetlands. The most depleted samples are from groundwater and Harp Lake which typically contain highly altered DOM. The δ34S-DOM and δ18O-DOM can provide valuable information on sources of DOM and DOM alteration within the catchment. The δ18O-DOM could also allow the separation of autochthonous and allochthonous DOM in lakes.

As an efficient filter of airborne Hg compounds, forests retain a significant portion of the Hg deposited to the land. Forested watersheds have been identified as major sources of low-concentration Hg compounds to surrounding streams and lakes. Mercury export from forests is highly variable in both space and time. It is difficult to use field surveys alone to capture the spatiotemporal variation inherent in this variable. Mathematical models are required for improved representation. The objective of this Thesis is to develop and test a monthly dynamic model that can be used to estimate seasonal Hg export from forested watersheds to low-ordered forest streams. The fully developed model consists of four model components: (i) a forest hydrology component, to simulate variation in soil temperature, soil moisture, and stream discharge for input to the other model components; (ii) a forest nutrient cycling and biomass growth component, to simulate forest growth and litter production; (iii) a forest litter decomposition component, to simulate seasonal production of dissolved organic carbon (DOC); and (iv) a monthly DOC and Hg export component to simulate the translocation of DOC and Hg from forested watersheds to low-ordered streams. The Hg-export component incorporates an Hg-to-DOC binding coefficient estimated from a one-time stream survey of Hg and DOC concentrations. Simulations of in-stream Hg concentrations show two main trends: (i) an annual trend, associated with the seasonal (monthly) dynamics of forest litter production, decomposition, and DOC production and export, and (ii) a multiple-year trend, associated with forest harvesting and re-growth patterns of regenerating forests. This study demonstrates that (i) wetland- and conifer-dominated watersheds release a greater amount of Hg to aquatic ecosystems than upland- and deciduous species-dominated watersheds, and (ii) forests nearing maturity, export more Hg than young forests.

This Thesis presents a framework for modelling species-specific site quality index (SQI) at a spatial resolution of 250 m by integrating biophysical variables of growing degree days (GDD), soil water content (SWC), and incident photosynthetically active radiation (PAR) in descriptions of potential tree growth. Development of GDD maps is based on processing and blending remotely-sensed data acquired with the Moderate Resolution Imaging Spectroradiometer (MODIS) sensor on the Terra satellite and ETM+ sensor on Landsat-7 satellite at spatial resolutions of 250 m and 28.5 m. Descriptions of SWC are based on a temperature-vegetation wetness index (TVWI) that relies on MODIS-based optical and thermal image products. PAR is estimated with an existing solar-radiation distribution model. SQI is defined as a function of species vital attributes and species environmental response to GDD, TVWI, and PAR. The methods are applied to a balsam fir [bF; Abies balsamea (L.) Mill.] dominated region in northwest New Brunswick. Comparisons between SQI and field-based estimates of site index and enhanced vegetation index showed that about 66 and 88% of the values corresponding to a series of Forest Development Survey lines (691 in total) were within 16% of SQI values. On average 92.1% of high bF-content stands (> 50% composition) in the area fell on medium-to-very high SQI values (> 0.50). Based on these agreements, SQI can be perceived as a good predictor of potential tree-species growth in the selection of optimal sites for biomass and wood fibre production.

Com o objetivo de compreender quais fatores abióticos determinam a ocorrência das diferentes formações florestais na região de Batatais, entre as coordenadas 20038S e 20058S e 47037W e 47028W, foram escolhidas três propriedades agrícolas (Fazenda Monte Belo, em Restinga, SP e Fazenda Magnólia e Sítio Pratinha, em Batatais, SP), que apresentavam remanescentes florestais representativos da região (floresta ribeirinha paludícula, floresta estacional semidecídua ribeirinha e cerradão). Para descrever a composição florística e a estrutura da comunidade arbórea foram amostradas 05 parcelas de 10 x 10 m ao redor de cada estação tensiométrica. Para o estudo dos fatores abióticos foram determinados os parâmetros físicos e químicos dos solos e as curvas de retenção de água nos solos e piezometria. A discriminação das variáveis abióticas e de vegetação foi feita através da análise de componentes principais (PCA) e, na correlação entre a abundância de espécies e fatores abióticos, foram feitas análises diretas de gradiente por meio de Análise de Correlação Canônica (CCA). A textura dos solos (teores de argila, silte e areia) e a posição no terreno (cota), foram as variáveis ambientais mais importantes na distribuição das espécies e nas variações das fisionomias florestais, com influência na retenção hídrica e nos índices de fertilidade dos solos. Essas diferenças nos teores de argila em profundidade ao longo do declive, com influência direta na retenção de umidade dos solos ou mesmo na formação de ambientes paludículos, determinaram grupos distintos de espécies e fisionomias florestais: (i) um grupo de espécies de ocorrência típica nas fisionomias de Cerrado e fortemente associado às cotas mais elevadas (solos menos férteis, bem drenados e porcentagens mais baixas de argila e silte nas camadas mais profundas), entre as quais: Matayba juglandifolia, Trichilia pallida, Lithraea molleoides, Anadenanthera falcata, Myrcia tomentosa, Copaifera langsdorffii, Erytrhoxylum pelleteranum, Platipodium elegans, Chrysophyllum marginatum, Luehea divaricata, entre outras e (ii) um grupo com espécies indicadoras de Florestas Paludículas ou de ocorrência comum nestas fisionomias, associado aos solos das cotas mais baixas (Gleissolos), com porcentagens altas de argila e silte nas camadas superficiais, mais férteis e maior retenção hídrica: Cecropia pachystachya, Citronella gongonha, Rapanea guianensis, Talauma ovata e Tapirira guianensis. Pode-se considerar um terceiro grupo de transição entre Floresta Estacional Semidecídua e Cerradão, nas cotas intermediárias e menos úmidas (espécies amostradas em parcelas localizadas nas bordas das Florestas Paludículas), onde ocorreram espécies tanto de ambiente de Cerradão, como de Floresta Estacional Semidecídua, e algumas poucas de ambiente paludículo. As porcentagens mais elevadas de Areia nos solos do Sítio Pratinha, com influência na menor retenção hídrica e também nos índices de fertilidade (solos álicos, com maior acidez e os menos férteis), podem explicar algumas diferenças na composição e abundância das espécies entre fisionomias florestais iguais. O Cerradão da Fazenda Monte Belo, mostrou-se diferente floristicamente do Cerradão do Sítio Pratinha. Esta mesma diferença ocorreu em relação às Florestas Paludículas da Fazenda Magnólia e a do Sítio Pratinha. As poucasespécies de ocorrência comum tiveram populações diferentes. A CCA evidenciou também correlações de algumas espécies com determinadas variáveis de solo. / In order to understand what abiotic factors determine the occurrence of different forest formations in the region of Batatais, coordinated between 20038\'S and 20058\'S and 47037\'W and 47028\'W, three agricultural properties were chosen (Monte Belo, in the city of Restinga, SP and Magnolia and Pratinha in Batatais, SP, Brazil), which had forests representative of the region (swamp forest, semi-deciduous riverine forest and forested savanna). To describe the floristic composition and structure of the arboreal community, 05 plots of 10 x 10 meters around each tensiometric station were sampled. For the study of abiotic factors, the physico-chemical characteristics of the soils, the soil-water-retention curves and the groundwater fluctuation were determined. The discrimination of the environmental variables and arboreal species were made through the Principal Components Analysis (PCA), and in the correlation between the abundance of species and abiotic factors, direct analyses of gradient through Canonical Correspondence Analysis (CCA) were made. The texture of the soil (levels of clay, silt and sand) and the position on the ground (altitude) were the most important environmental variables in the distribution of species and variations of forest physiognomies, with influence in the retention rates of water and soil fertility. These differences in levels of clay in depth along the slope, with direct influence on the retention of soil moisture and even in the development of swampy environment, determined distinct groups of species and forest physiognomies: (i) a group of species of typical occurrence in physiognomies of Forested Savanna and strongly associated with higher altitude (less fertile, well-drained soil and lower percentages of clay and silt in the deeper layers), including: Matayba juglandifolia, Trichilia pallida, Lithraea molleoides, Anadenanthera falcata, Myrcia tomentosa, Copaifera langsdorffii, Erytrhoxylum pelleteranum, Platipodium elegans, Chrysophyllum marginatum, Luehea divaricata, among others, and (ii) a group with indicator species of Swamp Forests or common occurrence in these physiognomies, associated with lower altitude (Gleisoil), with high percentages of clay and silt in the upper and more fertile layers, and greater water retention: Cecropia pachystachya, Citronella gongonha, Rapanea guianensis, Talauma ovata and Tapirira guianensis. A third group of transition between Semi-deciduous Forest and Forested Savanna, in intermediate altitude and soils less humid (species sampled in plots located at the edges of Swamp Forests), occurred both in the Forested Savanna and in Semi-deciduous Forest and a few in swampy environment. The highest percentages of sand in the soil of Pratinha, with less influence on water retention and also on the rates of fertility (alic soils, with greater acidity and less fertile), may explain some differences in the composition and abundance of species of forest physiognomies equal. The forested Savanna of Monte Belo, has proved floristically different from the Forested Savanna of Pratinha. This same difference occurred in swamp forests of Magnolia and Pratinha. The few species of common occurrence had differentpopulations. The CCA also revealed correlations of some species with certain variables of soil.

Modelling the wake effect generated by wind turbines is an essential part for calcu- lating a wind farm’s expected energy production. Operating wind turbines disturb the flow of the wind, which results in decreased production of downwind turbines. The N. O. Jensen model is an industry standard wake model that assumes a linear expansion of the downstream wake. The only adjustable parameter in the model is the wake decay constant (WDC), which has traditionally been derived semi em- pirically from terrain surface roughness. However, the WDC defines the expansion rate of the generated wake, and therefore can be linked to the ambient turbulence intensity (TI): high ambient turbulence leads to a faster decay of the generated wake, and therefore to lower wake losses, and vice-versa. Since the influence of the roughness on the ambient turbulence intensity is expected to be less significant at higher heights, these roughness-based WDC values are rather uncertain for the hub heights employed nowadays. The following study presents the results of a comparison between observed and mod- elled wake losses based on different WDC values. To investigate how a change in height affects the wake modelling, two wake scenarios occurring between two tur- bine sets with different hub heights are selected from an operational wind farm. By modelling the wakes using roughness as well as turbulence intensity-based WDCs, conclusions can be drawn on how the predictive capability of the N.O. Jensen model depends on the selection of a suitable WDC value. Finally it is concluded that the goodness of fit between modelled and observed wake losses shows a clear dependency on the wind speed/power production inter- val. At higher wind speeds, the TI-based WDC resulted in a better accuracy of the modelled wake losses as compared to the roughness-based WDC, while for lower wind speeds the N. O. Jensen model performed most accurately when using WDC = 0.075. However, for the investigated cases the overall accuracy of the modelled wake appears to be higher when choosing WDC = 0.075 instead of a TI-based WDC.

The field of Landscape Architecture can further develop a niche for the design of sustainable productive landscapes. This study attempts to understand a major stakeholders’ perceptions and use of riparian buffers and other conservation practices for water quality in an agricultural watershed of Mississippi. A survey was distributed to agricultural producers in the Big Sunflower Watershed of the MS Delta. The survey informs the interested parties of producers’ perceptions and uses of riparian forested buffers, perceptions and uses of conservation practices that restore water quality, perceptions of their environment, perceptions of surface water quality, enrollment of governmental incentive programs, and utilization of digital technology. Analysis of this data could lead to a better understanding of the knowledge and attitudes farmers have of the riparian systems and watershed processes at work within the region and factors that influence the farmers’ decisions of implementing conservation plans.

Vernal pools are small seasonally-ponded wetlands that provide crucial habitat for amphibian reproduction and support trophic levels beyond their boundaries. The Ridge and Valley physiographic province in Pennsylvania is known to have vernal pools, but a regional inventory and geomorphology assessment is needed. My research is split into two independent parts focusing on the higher elevation areas of this region to determine vernal pool distribution and characteristics. Vernal pools were mapped using a LiDAR based suitability model and leaf-off aerial imagery interpretation. Four terrain rasters derived from a 1-meter DEM (modified wind modified wind exposure, terrain surface convexity, topographic position index, and a multiresolution index of valley bottom flatness) were used in the suitability model. An analysis of variance (ANOVA) and Tukey's HSD test found a significant difference using the model between terrestrial (non-wetland) habitat and vernal pools. Photo interpretation and field surveying lead to an inventory of 1011 vernal pools. Geomorphology was assessed from 13 variables to determine the best for vernal pool prediction. Three variables were significant for the occurrence and frequency of vernal pools; saddles with higher surface area, 0.6 to 1.5 kilometers between the summits of parallel ridgelines, and the presence of periglacial related solifluction. Vernal pool distribution is greater than previously known and they occur in predictable settings. Further research should focus on how and where vernal pools form, their impact on water quality, role in forest ecology, and ways to legally protect them at the state level. / Master of Science / Vernal pools are seasonally-ponded wetlands that are very important for amphibian reproduction. The Appalachian Mountains of Pennsylvania are known to have vernal pools, but comprehensive inventory is lacking. My research consists of two parts that focus on the higher elevation areas and assess the distribution and qualities of the vernal pools. Vernal pools were mapped using a LiDAR based suitability model and leaf-off aerial imagery interpretation. Statistical analysis was completed to prove that there was a significant difference in terrain morphology between non-wetland habitat and vernal pools. This research resulted in a total inventory of 1011 vernal pools. Results found that vernal pools were likely occur in landscape positions with higher surface area, 0.6 to 1.5 kilometers between the summits of parallel ridgelines, and the presence of topographic features indicative of glacial processes. Vernal pools are much more abundant than previously known and they occur in predictable settings. Further research could focus on the formation of vernal pools, impact on water quality, role in forest ecology, and ways to legally protect them at the state level.

Bothrops bilineatus é uma das seis espécies presentes no grupo taeniatus, é reconhecida atualmente por duas sub-espécies: B. b. smaragdinus que se distribui pelo oeste Amazônico e B. b. bilineatus com distribuição disjunta pela Amazônia e Mata Atlântica. Este trabalho tem como objetivo testar a validade das duas sub-espécies com base em ferramentas moleculares, morfométricas, merísticas, hemipenianas e osteológicas, reconhecendo e delimitando as linhagens encontradas. Pretende-se ainda investigar as relações filogeográficas entre as populações ao longo da sua distribuição, analisando o tempo de divergência e os possíveis eventos associados a sua diversificação. Além disso, pretende-se comparar a variação molecular e hemipeniana de B. bilineatus com B. taeniatus (espécie filogeneticamente próxima) e as existentes nos outros grupos de espécies do gênero. Os resultados mostram que a variação genética e hemipeniana encontram se em diferentes níveis ao longo dos grupos de espécies em Bothrops, fornecendo informação relevante a nível intra e inter-específico. Foram recuperados seis clados estruturados para Bothrops bilineatus, representando quatro linhagens independentes e diagnosticáveis com base conjunta nos caracteres morfológicos, hemipenianos, osteológicos e moleculares: linhagem 1 - Amazônia central, basal as outras, linhagem 2 - Mata Atlântica+Escudo das Guianas externa às linhagens 3 e 4 Amazônia oeste parte sul e norte, respectivamente. Já para B. taeniatus quatro clados representando três linhagens foram recuperadas com bases nos caracteres moleculares e hemipenianos, porém se faz necessário buscar por mais caracteres morfológicos e osteológicos para melhor delimitar e diagnosticar as linhagens. As mudanças paleoclimáticas e geomorfológicas ocorridas na transição Terciário/Quaternário parecem ser os principais eventos a influenciar na diversificação das linhagens encontradas em Bothrops bilineatus e B. taeniatus / Bothrops bilineatus is one of six species in the taeniatus group, is now recognized by two subspecies: B. b. smaragdinus that distributes through western Amazon and B. b. bilineatus with disjunct distribution through Amazon and Atlantic Forest. This work aims to test the validity of these two sub-species, based on molecular, morphometric tools, meristic, osteological and hemipenial data, recognizing and delimiting lineages found. We also intend to investigate the phylogeographic relationships among populations throughout its distribution, analyzing the divergence time and possible events associated with it diversification. In addition, we intend to compare the molecular and hemipenial variation presented in B. bilineatus with B. taeniatus (phylogenetically close species) and those in the other groups of the genus. The genetic and hemipenial results show different levels of variation along the groups of Bothrops species, providing significant intra-and inter-specific information. Six clades structured to Bothrops bilineatus were recovered, representing four independent lineages diagnosable with joint based on morphology, hemipenial, osteological and molecular characters: lineage 1 - central Amazon, basal the others, lineage 2 - Atlantic Forest + Guayana Shield region recovered outside the sisters lineages 3 and 4 - western Amazon, southern part and western Amazon, northern part, respectively. As for B. taeniatus four clades representing three lineages were recovered with bases in molecular and hemipenial characters, however it is necessary to search for more morphological and osteological characters to better delimit and diagnose the lineages. Paleoclimatic and geomorphic changes in the transition Tertiary/Quaternary, probably are the major events influencing the diversification of lineages found in Bothrops bilineatus and B. taeniatus

Forested watersheds play a dominant role in the global hydrological cycle. Very few experimental observatories especially in tropical forested regions of India have been undertaken. This study has been initiated for this reason and to gain insights into functioning of the hydrological system in such climatic conditions. This study involves experimental setup of a watershed, it’s monitoring till date, modelling of the hydrological processes observed and the challenges in modelling components of the water balance in this watershed. A Small Experimental Watershed of 4.3 Km2 was set up at Mule Hole, in South India along the Kerala-Karnataka State borders, and is situated inside the Bandipur National park. After an overview of watershed studies, review of literature related to forest watershed studies and processes in the first two chapters, Chapter 3 introduces the study area, Mule Hole Experimental Watershed and explains the methodology used to study this watershed. Model SWAT was used initially to simulate the water balance components. A brief description of the model, methodology adopted and discussion on the results obtained is presented in Chapter 4. The watershed initially modelled as an ungauged watershed using the default parameters in the model, simulated very high groundwater contribution to the runoff. The calibrated model although performed favourably for annual average values and monthly calibration, the daily calibration was unsatisfactory. An auxiliary study on quantification of actual and potential evapotranspiration (ET0) has been carried out in Chapter 5 . Ten methods including Penman-Montieth were compared and evaluated for efficacy of the methods. All methods except for Hargreaves method showed agreement with the Penman-Montieth for annual average values. Priestly-Taylor method was found be the best estimator in comparison with Penman-Montieth method, when used to estimate AET. Adjusted Hargreaves and FAO Blaney -Criddle method were found to be very useful when few or limited climatic data were available for estimation of Potential evapotranspiration. A multidisciplinary approach of estimating recharge consisting of chloride mass balance technique coupled with study of water table fluctuations and groundwater flow analytical modelling has been attempted in Chapter 6. Direct and localized recharge was estimated at 45 mm/yr and indirect recharge 30 mm/yr for the monitored years in the watershed. The low values of recharge rates implied an unexpected very high evapotranspiration rate. It may be inferred that in the absence of groundwater flow to the stream, the recharge joins groundwater flow as outflow of the hydrologic system. An integrated lumped model incorporating the regolith zone and the capability of the tree roots to access this store is presented in Chapter 7. The model was able to simulate the pattern of lag-time between water table rise was observed in shallow piezometers in comparison with hillslope piezometers. The patterns of water table variation among the different hillslope piezometers suggest that they are linked with local processes and not by a regional aquifer dynamics. This study shows that water uptake, combined with the spatial variability of regolith depth, can account for the variable lag time between drainage events and groundwater rise observed for the different piezometers. Chapter 8 discusses the results, conclusions derived from this study and possibility of further scope of studies.