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Achieving energy neutrality has shifted focus towards aeration systems optimization, due to the high energy consumption of aeration processes in modern advanced wastewater treatment plants. The activated sludge wastewater treatment process is dependent on aeration efficiency which supplies the oxygen needed in the treatment process. The process is a complex heterogeneous mixture of microorganisms, bacteria, particles, colloids, natural organic matter, polymers and cations with varying densities, shapes and sizes. These activated sludge parameters have different impacts on aeration efficiency defined by the OTE, % and alpha. Oxygen transfer efficiency (OTE) is the mass of oxygen transferred into the liquid from the mass of air or oxygen supplied, and is expressed as a percentage (%). OTE is the actual operating efficiency of an aeration system. The alpha Factor (α) is the ratio of standard oxygen transfer efficiency at process conditions (αSOTE) to standard oxygen transfer efficiency of clean water (SOTE). It is also referred to as the ratio of process water volumetric mass transfer coefficient to clean water volumetric mass transfer coefficient. The alpha factor accounts for wastewater contaminants (i.e. soap and detergent) which have an adverse effect on oxygen transfer efficiency. Understanding their different impacts and how different treatment technologies affect aeration efficiency will help to optimize and improve aeration efficiency so as to reduce plant operating costs. A pilot scale study of fine pore diffuser fouling and mitigation, quantified by dynamic wet pressure (DWP), oxygen transfer efficiency and alpha measurement were performed at Blue Plains, Washington DC. In the study a mechanical cleaning method, reverse flexing (RF), was used to treat two diffusers (RF1, RF2) to mitigate fouling, while two diffusers were kept as a control with no reverse flexing. A 45 % increase in DWP of the control diffuser after 17 month of operation was observed, an indication of fouling. RF treated diffusers (RF1 and RF2) did not show any significant increase in DWP, and in comparison to the control diffuser prevented a 35 % increase in DWP. Hence, the RF fouling mitigation technique potentially saved blower energy consumption by reducing the pressure burden on the air blower and the blower energy requirement. However, no significant impact of the RF fouling mitigation treatment technique in preventing a decrease in alpha-fouling (𝝰F) of the fine pore diffusers over time of operation was observed. This was because either the RF treatment method maintained wide pore openings after cleaning over time, or a dominant effect of other wastewater characteristics such as the surfactant concentration or particulate COD could have interfered with OTE. Further studies on the impact of wastewater characteristics (i.e., surfactants and particulate COD) and operating conditions on OTE and alpha were carried out in another series of pilot and batch scale tests. In this study, the influence of different wastewater matrices (treatment phases) on oxygen transfer efficiency (OTE) and alpha using full-scale studies at the Blue Plains Treatment Plant was investigated. A strong relationship between the wastewater matrices with oxygen transfer characteristics was established, and as expected increased alphas were observed for the cleanest wastewater matrices (i.e., with highest effluent quality). There was a 46 % increase in alpha as the total COD and surfactant concentrations decreased from 303 to 24 mgCOD/L and 12 to 0.3 mg/L measured as sodium dodecyl sulphate (SDS) in the nitrification/denitrification effluent with respect to the raw influent. The alpha improvement with respect to the decrease in COD and surfactant concentration suggested the impact of one or more of the wastewater characteristics on OTE and alpha. Batch testing conducted to characterize the mechanistic impact of the wastewater contaminants present in the different wastewater matrices found that the major contaminants influencing OTE and alpha were surfactants and particulate/colloidal material. The volumetric mass transfer coefficient (kLa) measurements from the test also identified surfactant and colloidal COD as the major wastewater contaminants present in the influent and chemically enhanced primary treatment (CEPT) effluent wastewaters impacting OTE and alpha. Soluble COD was observed to potentially improve OTE and alpha due to its contribution in enhancing the oxygen uptake rate (OUR). Although the indirect positive impact of OUR on alpha observed in this study contradicts some other studies, it shows the need for further investigation of OUR impacts on oxygen transfer. Importantly, the mechanistic characterization and quantitative correlation between wastewater contaminants and aeration efficiency found in this study will help to minimize overdesign with respect to aeration system specification, energy wastage, and hence the cost of operation. This study therefore shows new tools as well as the identification of critical factors impacting OTE and alpha in addition to diffuser fouling. Gas transfer depression caused by surfactants when they accumulate at the gas-liquid interface during the activated sludge wastewater treatment process reduces oxygen mass transfer rates, OTE and alpha which increases energy cost. In order to address the adverse effect of surfactants on OTE and alpha, another study was designed to evaluate 4 different wastewater secondary treatment strategies/technologies that enhances surfactant removal through enhanced biosorption and biodegradation, and to also determine their effect on oxygen transfer and alpha. A series of pilot and batch scale tests were conducted to compare and correlate surfactant removal efficiency and alpha for a) conventional high-rate activated sludge (HRAS), b) optimized HRAS with contactor-stabilization technology (HRAS-CS), c) optimized HRAS bioaugmented (Bioaug) with nitrification sludge (Nit S) and d) optimized bioaugmented HRAS with an anaerobic selector phase technology (An-S) reactor system configuration. The treatment technologies showed surfactant percentage removals of 37, 45, 61 and 87 %, and alphas of 0.37 ±0.01, 0.42 ±0.02, 0.44 ±0.01 and 0.60 ±0.02 for conventional HRAS, HRAS-CS, Bioaug and the An-S reactor system configuration, respectively. The optimized bioaugmented anaerobic selector phase technology showed the highest increased surfactant removal (135 %) through enhanced surfactant biosorption and biodegradation under anaerobic conditions, which also complemented the highest increased alpha (62 %) achieved when compared to the conventional HRAS. This study showed that the optimized bioaugmented anaerobic selector phase reactor system configuration is a promising technology or strategy to minimize the surfactant effects on alpha during the secondary aeration treatment stage / Ph. D.