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The issue of phosphorus in sediments of small watercourses and phosphorus forms are often out of interest, unlike phosphorus and its forms in sediments of water reservoirs. The thesis is focused on the development and evaluation of methods for the determination of total phosphorus by modifying commonly used methods and evaluation of methods for the determination of selected forms of phosphorus in small watercourses sediments. Over 1,152 analyses of sediment samples collected in four seasons and four locations and in three paralell determinations were done. The total phosphorus in the sediments was assessed by four methods, further 7 other forms of phosphorus were evaluated by one to three methods, according to different forms of phosphorus. The data file was subjected to statistical analysis. The aim of statistical analysis was to evaluate phosphorus concentrations, depending on the used method. Additional aim of statistical analysis was to observe dependencies of phosphorus concentrations on metals and identify the impact of periods and locations on the concentration of phosphorus.