The objectives of this research were to compare lying time, milk yield, rumination time, hock score, stall cleanliness, and stall temperature for 97 cows (Holsteins (n = 71), Jerseys (n = 10), and crossbreds (n = 16)) housed on Dual Chamber Cow Waterbeds™ (DCCW, Advanced Comfort Technology, Reedsburg, WI) or conventional rubber mattresses (MAT). This study was conducted at the University of Kentucky Coldstream Research Dairy Farm from January 18, 2012 to May 3, 2013. Lying times were longer (P < 0.01) for cows housed on the DCCW (10:32 ± 0:13) compared to cows housed on the MAT (9:47 ± 0:15). Milk yield was not different (P ≥ 0.05) between cows housed on the DCCW or MAT. Rumination times were greater (P = 0.03) for cows housed on MAT (6:44 ± 0:08) compared to cows housed on DCCW (6:29 ± 0:08). Hock scores were lesser (P = 0.02) for cows housed on DCCW (1.86 ± 0.03) compared to cows housed on MAT (1.97 ± 0.04). Stall cleanliness was not different (P ≥ 0.05) between the DCCW and the MAT. The DCCW stall temperature was warmer (P < 0.01) (13.29 ± 0.21 ⁰C) than the MAT (10.52 ± 0.21 ⁰C).