The motivational properties of Longstreth's (1970) definitions of incentive and frustrative cues were tested using 32 rats in a two phase straight alleyway experiment. During pretraining, incentive cue Ss were presented a visual cue prior to reinforcement; frustrative cue Ss experienced the visual cue simultaneously with reinforcement. Ss encountered the same cue in mid-alley during 40 CRF training trials. Significant inhibition developed as frustrative cue Ss passed through the cue and postcue segments. Significant incentive effects occurred midway through training only in the postcue segment. Differential resistance to extinction was not found. The results did not support all of Longstreth's assumed functions. The motivational effects were interpreted using Spence's and Amsel's instrumental learning paradigms.