Fumaria officinalis es una maleza importante del cultivo de trigo en el sur y sudoeste de la provincia de Buenos Aires. Durante los últimos años ha incrementado su presencia en los partidos de Villarino y Patagones, tanto en sistemas de labranza convencional como conservacionista, aumentando su densidad poblacional y la superficie ocupada dentro de los cultivos de trigo. Fueron estudiados algunos aspectos sobre la bioecología y el manejo de F. officinalis en el cultivo de trigo dentro del agroecosistema regional. Se formula la hipótesis de que un mayor conocimiento de la dinámica de emergencia de la maleza en el campo, su competencia con el cultivo y del control con diferentes herbicidas ayudará a un manejo más eficiente y sustentable de la maleza en la región. Se estudió el patrón de emergencia de la maleza, así como la influencia que sobre el proceso tiene la remoción del suelo (labranzas otoñales), la profundidad de entierro y la compactación del suelo. Paralelamente fue estudiada la competencia cultivo-maleza, evaluando el efecto que tiene la fertilización nitrogenada, el momento de emergencia relativo y la densidad de la maleza. También se evaluaron herbicidas postemergentes selectivos de trigo en diferentes momentos fenológicos del cultivo y se estudió la respuesta de F. officinalis a diferentes dosis del herbicida glifosato en dos estadios fenológicos de la maleza. Los resultados en cuanto a la emergencia de la maleza en el campo, indican que se produce en el otoño e invierno, y se estimula principalmente por las precipitaciones y las labranzas del suelo. En la interferencia cultivo maleza, F. officinalis demostró ser un buen competidor por nitrógeno del suelo frente al trigo. La competencia que ejerce la maleza sobre el cultivo es baja y los mayores perjuicios se observan cuando la emergencia ocurre antes de las 2-3 hojas del cultivo y en altas densidades de la maleza. En cuanto al control químico en postemergencia de trigo, los herbicidas prosulfurón + triasulfurón e iodosulfurón + metsulfurón tienen muy buena efectividad sobre la maleza. El herbicida glifosato tiene muy buena acción de control inclusive en dosis bajas, dependiendo del estado fenológico de las plantas. Los resultados sugieren la realización de un manejo sustentable de F. officinalis, adelantando la fecha de siembra del cultivo, monitoreando las densidades de infestación y reduciendo las dosis de aplicación de herbicidas según el estado fenológico de la maleza. / Fumaria officinalis is an important weed of wheat crops in the south and southwest of Buenos Aires province. During the past few years its presence in conventional as well as in conservationist farming systems has increased in the area of Villarino and Patagones, increasing its population density and the surface occupied within wheat. Some aspects of the ecology and management of F. officinalis in wheat crops within agro-ecosystems of the south-southwest of Buenos Aires province were studied. The hypothesis formulated is that a greater knowledge of the emergence dynamics the weed in the field, of its competition with the culture, and of the degree of control by different herbicides will help to a more efficient and sustainable handling of this weed in the region. The pattern of emergence of the weeds was studied, as well as the influence that the following factors have on the process: 1) Soil disruption (autumn tillage), 2) Depth and 3) Compaction of the ground. Crop - weed competition was studied, evaluating the effect of nitrogen fertilization, the relative moment of emergence and the density of the weeds. Also, selective post-emergent herbicides applied at different phenological stages of the crop were evaluated, and the answer of F. officinalis to different doses of glyphosate herbicide at two phenological stages of the weed was studied. Results, indicate that the emergence of the weeds in the field, takes place in autumn and winter, and is mainly stimulated by precipitations and soil tillage. Regarding crop - weed interference, F. officinalis competed well against wheat for the nitrogen of the soil. The competition that exerts the weed on the culture is low and the greatest damages are observed when the emergence happens before the 2-3 leaves stage of the crop, and with high densities of the weed. Regarding chemical control at post-emergence of wheat, the herbicides prosulfurón + triasulfurón and iodosulfurón + metsulfurón have very good effectiveness against the weed. Depending on the phenological state of the plants, the herbicide glyphosate has very good effect on the weed, even at low doses. Considering all these results, a sustainable management of F. officinalis is proposed, advancing the date of sowing of the culture, monitoring weed densities and reducing the application doses of herbicides according to the phenological state of the weed.

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Daniela Hulcová Consultant: Prof. RNDr. Lubomír Opletal, CSc. Title of Diploma Thesis: Biological aktivity of plants metabolites. XXVII. Alkaloids of Fumaria officinalis L. and their effect on acetylcholinesterase and butyrylcholinesterase The summary ethanolic and diethylether extract were prepared from the herbs of a plant Fumaria officinalis L. We have obtained 201 fractions from this extract by column chromatography on the neutral Al2O3 (aluminium oxide). Joined fraction 68-76 were processed by thin layer chromatography, and 3 substances were obtained in pure state: DH-1, DH-2, DH-3. These 3 compounds were identified as protopine, (+)-fumariline and N- methylcorydaldine by the comparison with the literature and results of MS and NMR. These alkaloids were tested for the inhibitory activity against human erythrocytic acetylcholinesterase and plasmatic butyrylcholinesterase by Ellman`s method. The isolated alkaloids did not show any significant inhibitory activity (IC50, µM) compared with the standard galanthamine (IC50, µM; AChE 1,710 ± 0,065, BuChE 42,30 ± 1,30): protopin: AChE: 345,42 ± 31,12, BuChE: 239,66 ± 20,89, (+)-fumarilin: AChE: 2939,2 ± 309,41, BuChE: 330,62 ±...

Malý, L.: Study of biological activity of alkaloids isolated from Fumaria officinalis L. (Fumariaceae) II. Diploma Thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2014, 49 pp. Obtained diethylether extract of Fumaria officinalis L. was separated to fractions in column chromatography with petrol, chloroform and ethanol. Preparative TLC and crystalisation led to isolation of five alkaloids from fraction. Alkaloids were identified by GC-MS and NMR specters, optical rotation and melting point as protopine, cryptopine, (-)-fumaricine, (+)-fumariline and (+)-parfumidine. Isolated alkaloids were tested for their inhibition activity towards acetyl- and butyrylcholinesterase and towards prolyloligopeptidase. Activities were compared with standards. Natural inhibitor galanthamine showed IC50 AChE 1.710 ± 0.065 µM, IC50 BuChE 42.30 ± 1.30 µM. Best inhibition activity showed protopine (IC50 AChE 345.4 ± 24 µM, IC50 BuChE 239.6 ± 22.3 µM) and cryptopine (IC50 AChE 477.71 ± 47.33 µM, IC50 BuChE 270.82 ± 39.12 µM). The highest prolyloligopeptidase inhibition activity showed (+)-parfumidine with IC50 POP 99.2 µM, which was more active than used natural inhibitor baicaline (IC50 POP 605.9 ± 0.021 µM). Synthetic POP...

Kostelník, J.: Study of biological activity of alkaloids isolated from Fumaria officinalis L. (Fumariaceae) I. Diploma thesis, Charles University in Prague,Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2014, 63 p. The aim of this study was to isolate alkaloids from joined fraction no. 55-67 (A2) obtained from the total alkaloid fraction of extract of Fumaria officinalis L. (Fumariaceae) plant. Using chromatography methods three alkaloids were isolated and then identified by structural analysis (GC-MS, NMR). Three alkaloids were isolated by using common chromagografic methods and then identified by structural analyses optical rotation and melting point as (-)-O- methylfumarophycine, (-)-sinactine a (-)-stylopine. Inhibitory activity of isolated alkaloids was assessed against human erythrocyte acetylcholinesterase, human butyrylcholineesterase and prolyl oligopeptidase. The results were expressed as IC50 values ((-)-stylopine: IC50 AChE and IC50 BuChE > 1000 μM, IC50 POP > 1000 mM; (-)-O-methylfumarophycine: IC50 AChE = 963.10 ± 135.98 µM, IC50 BuChE = 1771.0 ± 380.94 µM, IC50 POP - unmeasured; (-)-sinactine IC50 AChE = 632.0 ± 68.12 µM, IC50 BuChE = 8154.3 ± 981.42 µM, IC50 POP = IC50 POP = 52.9 ± 1.8 µM). None of alkaloids isolated showed...