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A study of transformation in Leptosphaeria maculansFarman, Mark January 1990 (has links)
No description available.
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Chemical investigations of freshwater and fungicolous fungiJiao, Ping. January 2006 (has links)
Thesis (Ph.D.)--University of Iowa, 2006. / Supervisor: James B. Gloer. Includes bibliographical references (leaves 232-238).
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Cell-wall mechanical properties of Saccharomyces cerevisiae /Smith, Alexander Evans. January 1999 (has links) (PDF)
Thesis (Ph.D.)--University of Adelaide, Dept. of Chemical Engineering, 1999. / Bibliography: leaves 182-190.
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Investigation of quorum sensing in C. albicansShchepin, Roman V. January 1900 (has links)
Thesis (Ph.D.)--University of Nebraska-Lincoln, 2006. / Title from title screen (site viewed April 27, 2007). PDF text: vi, 165 p. : ill. (some col.) UMI publication number: AAT 3233745. Includes bibliographical references. Also available in microfilm and microfiche formats.
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The utilization of second generation feedstocks for the production of platform chemicals by filamentous fungiHu, Ziyi 05 October 2012 (has links)
The depletion of petroleum and other platform chemical resources are a global concern;
therefore alternative substrates must be identified to replace these current sources. Thus
allowing research in fungal biotechnology to prosper, as filamentous fungi can utilize
second-generation feedstocks or agricultural waste to produce these petroleum derived
platform chemicals. This research focuses on the ability of filamentous fungi to use
different second-generation feedstocks such as wheat bran and sugar cane bagasse to
generate platform chemicals of interest, namely being itaconic acid (IA) and other
organic acids of interest, such as citric acid. This study focused on the metabolite
producing capabilities of Aspergillus terreus, initially in a shake flask fermentation
environment and then in an Airlift Bioreactor environment utilizing hydrolyzed wheat
bran and sugar cane bagasse as a substrate source to produce metabolites of interest. The
initial shake flask fermentation experiment involved inoculation and incubating A.
terreus in hydrolyzed wheat bran with additional minerals at 30°C for 5 days at a pH
range of between 3-4. The result yielded itaconic acid and citric acid concentrations of
1.01g/l and 6.23g/l at their peaks, respectively. The airlift bioreactor was run for 16 days
with a constant pH range between 3-4, at a temperature of 30°C with a dissolved oxygen
level of 20g/l. The result of the study yielded a high itaconic acid and citric acid
concentration peaking at 59.4 g/l and 59.2 g/l, respectively.
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Cell-wall mechanical properties of Saccharomyces cerevisiae / by Alexander Evans Smith.Smith, Alexander Evans January 1999 (has links)
Bibliography: leaves 182-190. / xv, 190 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Develops a suitable approach to determining the fundamental cell-wall mechanical properties of single biological cells, by combining the single-cell compression experiment with a mechanical model to determine the cell-wall mechanical properties of the yeast Saccharomyces cerevisiae / Thesis (Ph.D.)--University of Adelaide, Dept. of Chemical Engineering, 1999
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Biotin protein ligase from Saccharomyces cerevisiae /Polyak, Steven William. January 2000 (has links) (PDF)
Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 2000? / Bibliography: leaves 130-159.
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"Characterization of a small ribozyme with self-splicing activity"Harris, Lorena B. January 2008 (has links)
Thesis (Ph.D.)--Bowling Green State University, 2008. / Document formatted into pages; contains x, 126 p. : ill. Includes bibliographical references.
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Catalytic mechanism of Saccharomyces cerevisiae NAD+-dependent 5,10-methylenetetrahydrofolate dehydrogenaseWagner, Wendi Suzanne, Robertus, Jon D. January 2004 (has links) (PDF)
Thesis (Ph. D.)--University of Texas at Austin, 2004. / Supervisor: Jon Robertus. Vita. Includes bibliographical references.
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Molecular studies on the Chinese straw mushroom, volvariella volvacea.January 1994 (has links)
by Chen Ming-jie. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 81-95). / List of Abbreviations --- p.I / List of Tables --- p.II / List of Figures --- p.III / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Background of Volvariella volvacea and the purposes of this study --- p.1 / Chapter 1.1.1 --- Background of Volvariella volvacea --- p.1 / Chapter 1.1.2 --- Purposes of this molecular study on Volvariella volvacea --- p.5 / Chapter 1.2 --- Molecular studies in edible mushrooms --- p.5 / Chapter 1.2.1 --- Recombinant DNA technology --- p.5 / Chapter 1.2.2 --- Restriction fragment length polymorphisms (RFLPs) --- p.6 / Chapter 1.2.3 --- Polymerase chain reaction (PCR) --- p.7 / Chapter 1.2.3.1 --- Ribosomal RNA gene-PCR (rDNA-PCR) --- p.8 / Chapter 1.2.3.2 --- Random amplified DNAs by polymerase chain reaction --- p.10 / Chapter 1.2.3 --- Pulsed field gel electrophoresis --- p.12 / Chapter Chapter 2 --- Materials and Methods --- p.17 / Chapter 2.1 --- Organisms --- p.17 / Chapter 2.2 --- Cell cultivation and maintenance --- p.17 / Chapter 2.3 --- Solutions and chemicals --- p.17 / Chapter 2.3.1 --- Solutions for DNA extraction --- p.17 / Chapter 2.3.2 --- Solutions for agarose gel electrophoresis --- p.18 / Chapter 2.3.3 --- Solutions for DNA labeling and detection --- p.18 / Chapter 2.3.3.1 --- Colorimetry --- p.18 / Chapter 2.3.3.2 --- Chemiluminescence --- p.19 / Chapter 2.3.4 --- Hybridization solution --- p.19 / Chapter 2.3.5 --- PCR primers --- p.19 / Chapter 2.3.6 --- SOC medium --- p.20 / Chapter 2.4 --- Agarose gel electrophoresis --- p.20 / Chapter 2.5 --- DNA extraction and purification --- p.20 / Chapter 2.5.1 --- Genomic DNAs --- p.20 / Chapter 2.5.2 --- Plasmid DNA --- p.21 / Chapter 2.6 --- Formation of complementary ends --- p.23 / Chapter 2.6.1 --- Partial digestion of genomic DNA with the restriction enzyme Sau3A I --- p.23 / Chapter 2.6.2 --- Production of vector arms --- p.23 / Chapter 2.7 --- Ligation --- p.24 / Chapter 2.8 --- Transformation --- p.24 / Chapter 2.8.1 --- Chemical transformation method --- p.24 / Chapter 2.8.1.1 --- Preparation of competent E. coli cells --- p.24 / Chapter 2.8.1.2 --- Transformation --- p.25 / Chapter 2.8.2 --- Electroporation --- p.25 / Chapter 2.8.2.1 --- Preparation of electro-competent cells --- p.25 / Chapter 2.8.2.2 --- Electroporation --- p.26 / Chapter 2.9 --- Southern transfer and hybridization using non- radioactive method --- p.27 / Chapter 2.9.1 --- Random labeling the V.volvacea genomic DNA by digoxigenin-11-dUTP --- p.28 / Chapter 2.9.2 --- Conventional PCR to amplify and label cloned DNA inserts --- p.28 / Chapter 2.9.3 --- Southern blotting --- p.29 / Chapter 2.9.4 --- Prehybridization --- p.29 / Chapter 2.9.5 --- Hybridization --- p.30 / Chapter 2.9.6 --- High stringency washing --- p.30 / Chapter 2.9.7 --- Detection --- p.30 / Chapter 2.9.7.1 --- Color detection --- p.30 / Chapter 2.9.7.2 --- Chemiluminescent detection --- p.31 / Chapter 2.9.8 --- Reprobing --- p.31 / Chapter 2.9.9 --- Colony hybridization --- p.31 / Chapter 2.10 --- Polymerase chain reaction (PCR) --- p.32 / Chapter 2.10.1 --- Arbitrarily primed polymerase chain reaction (AP- PCR) --- p.32 / Chapter 2.10.2 --- Random amplification of polymorphic DNA (RAPD) --- p.32 / Chapter 2.10.3 --- Amplification of ribosomal RNA gene (rDNA- PCR) --- p.33 / Chapter 2.11 --- Pulsed field gel electrophoresis --- p.33 / Chapter 2.11.1 --- Preparation of protoplasts --- p.33 / Chapter 2.11.2 --- Embedding of chromosomal DNAs --- p.34 / Chapter 2.11.3 --- Electrophoresis --- p.34 / Chapter 2.11.4 --- Southern blotting and hybridization --- p.35 / Chapter Chapter 3 --- Results --- p.36 / Chapter 3.1 --- Construction of a partial genomic library for Volvariella volvacea --- p.36 / Chapter 3.1.1 --- Genomic DNA purification and restriction enzyme digestion --- p.36 / Chapter 3.1.2 --- Preparation of vector arms --- p.36 / Chapter 3.1.3 --- Ligation and transformation --- p.36 / Chapter 3.2 --- Characterization of clones in the genomic library --- p.42 / Chapter 3.3 --- Fishing out ribosomal RNA gene from the genomic library by homologous rDNA probe --- p.45 / Chapter 3.4 --- Strain typing --- p.50 / Chapter 3.4.1 --- Strain typing by RFLPs using moderately repetitive probes --- p.50 / Chapter 3.4.2 --- Strain typing by PCR-based protocols: AP-PCR and RAPD --- p.50 / Chapter 3.4.3 --- Strain typing by PCR- RFLPs --- p.56 / Chapter 3.5 --- Electrophoretic karyotype analysis by pulsed field gel electrophoresis --- p.56 / Chapter 3.5.1 --- Protoplast preparation --- p.56 / Chapter 3.5.2 --- The electrophoresis condition --- p.56 / Chapter 3.5.3 --- Southern hybridization --- p.65 / Chapter Chapter 4 --- Discussion --- p.68 / Chapter 4.1 --- Genomic library --- p.68 / Chapter 4.2 --- Generation of molecular markers --- p.70 / Chapter 4.2.1 --- RFLPs --- p.70 / Chapter 4.4.2 --- AP-PCR and RAPD methods --- p.71 / Chapter 4.2.3 --- PCR- RFLP of rRNA gene --- p.72 / Chapter 4.2.4 --- Comparison of the four types of molecular markers --- p.72 / Chapter 4.3 --- Electrophoretic karyotype by PFGE --- p.74 / Conclusion --- p.80 / References --- p.81
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