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Thesis (Ph.D.)--University of Iowa, 2006. / Supervisor: James B. Gloer. Includes bibliographical references (leaves 232-238).

Thesis (Ph.D.)--University of Adelaide, Dept. of Chemical Engineering, 1999. / Bibliography: leaves 182-190.

Thesis (Ph.D.)--University of Nebraska-Lincoln, 2006. / Title from title screen (site viewed April 27, 2007). PDF text: vi, 165 p. : ill. (some col.) UMI publication number: AAT 3233745. Includes bibliographical references. Also available in microfilm and microfiche formats.

The depletion of petroleum and other platform chemical resources are a global concern; therefore alternative substrates must be identified to replace these current sources. Thus allowing research in fungal biotechnology to prosper, as filamentous fungi can utilize second-generation feedstocks or agricultural waste to produce these petroleum derived platform chemicals. This research focuses on the ability of filamentous fungi to use different second-generation feedstocks such as wheat bran and sugar cane bagasse to generate platform chemicals of interest, namely being itaconic acid (IA) and other organic acids of interest, such as citric acid. This study focused on the metabolite producing capabilities of Aspergillus terreus, initially in a shake flask fermentation environment and then in an Airlift Bioreactor environment utilizing hydrolyzed wheat bran and sugar cane bagasse as a substrate source to produce metabolites of interest. The initial shake flask fermentation experiment involved inoculation and incubating A. terreus in hydrolyzed wheat bran with additional minerals at 30°C for 5 days at a pH range of between 3-4. The result yielded itaconic acid and citric acid concentrations of 1.01g/l and 6.23g/l at their peaks, respectively. The airlift bioreactor was run for 16 days with a constant pH range between 3-4, at a temperature of 30°C with a dissolved oxygen level of 20g/l. The result of the study yielded a high itaconic acid and citric acid concentration peaking at 59.4 g/l and 59.2 g/l, respectively.

Bibliography: leaves 182-190. / xv, 190 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Develops a suitable approach to determining the fundamental cell-wall mechanical properties of single biological cells, by combining the single-cell compression experiment with a mechanical model to determine the cell-wall mechanical properties of the yeast Saccharomyces cerevisiae / Thesis (Ph.D.)--University of Adelaide, Dept. of Chemical Engineering, 1999

Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 2000? / Bibliography: leaves 130-159.

Thesis (Ph.D.)--Bowling Green State University, 2008. / Document formatted into pages; contains x, 126 p. : ill. Includes bibliographical references.

Thesis (Ph. D.)--University of Texas at Austin, 2004. / Supervisor: Jon Robertus. Vita. Includes bibliographical references.

by Chen Ming-jie. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 81-95). / List of Abbreviations --- p.I / List of Tables --- p.II / List of Figures --- p.III / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Background of Volvariella volvacea and the purposes of this study --- p.1 / Chapter 1.1.1 --- Background of Volvariella volvacea --- p.1 / Chapter 1.1.2 --- Purposes of this molecular study on Volvariella volvacea --- p.5 / Chapter 1.2 --- Molecular studies in edible mushrooms --- p.5 / Chapter 1.2.1 --- Recombinant DNA technology --- p.5 / Chapter 1.2.2 --- Restriction fragment length polymorphisms (RFLPs) --- p.6 / Chapter 1.2.3 --- Polymerase chain reaction (PCR) --- p.7 / Chapter 1.2.3.1 --- Ribosomal RNA gene-PCR (rDNA-PCR) --- p.8 / Chapter 1.2.3.2 --- Random amplified DNAs by polymerase chain reaction --- p.10 / Chapter 1.2.3 --- Pulsed field gel electrophoresis --- p.12 / Chapter Chapter 2 --- Materials and Methods --- p.17 / Chapter 2.1 --- Organisms --- p.17 / Chapter 2.2 --- Cell cultivation and maintenance --- p.17 / Chapter 2.3 --- Solutions and chemicals --- p.17 / Chapter 2.3.1 --- Solutions for DNA extraction --- p.17 / Chapter 2.3.2 --- Solutions for agarose gel electrophoresis --- p.18 / Chapter 2.3.3 --- Solutions for DNA labeling and detection --- p.18 / Chapter 2.3.3.1 --- Colorimetry --- p.18 / Chapter 2.3.3.2 --- Chemiluminescence --- p.19 / Chapter 2.3.4 --- Hybridization solution --- p.19 / Chapter 2.3.5 --- PCR primers --- p.19 / Chapter 2.3.6 --- SOC medium --- p.20 / Chapter 2.4 --- Agarose gel electrophoresis --- p.20 / Chapter 2.5 --- DNA extraction and purification --- p.20 / Chapter 2.5.1 --- Genomic DNAs --- p.20 / Chapter 2.5.2 --- Plasmid DNA --- p.21 / Chapter 2.6 --- Formation of complementary ends --- p.23 / Chapter 2.6.1 --- Partial digestion of genomic DNA with the restriction enzyme Sau3A I --- p.23 / Chapter 2.6.2 --- Production of vector arms --- p.23 / Chapter 2.7 --- Ligation --- p.24 / Chapter 2.8 --- Transformation --- p.24 / Chapter 2.8.1 --- Chemical transformation method --- p.24 / Chapter 2.8.1.1 --- Preparation of competent E. coli cells --- p.24 / Chapter 2.8.1.2 --- Transformation --- p.25 / Chapter 2.8.2 --- Electroporation --- p.25 / Chapter 2.8.2.1 --- Preparation of electro-competent cells --- p.25 / Chapter 2.8.2.2 --- Electroporation --- p.26 / Chapter 2.9 --- Southern transfer and hybridization using non- radioactive method --- p.27 / Chapter 2.9.1 --- Random labeling the V.volvacea genomic DNA by digoxigenin-11-dUTP --- p.28 / Chapter 2.9.2 --- Conventional PCR to amplify and label cloned DNA inserts --- p.28 / Chapter 2.9.3 --- Southern blotting --- p.29 / Chapter 2.9.4 --- Prehybridization --- p.29 / Chapter 2.9.5 --- Hybridization --- p.30 / Chapter 2.9.6 --- High stringency washing --- p.30 / Chapter 2.9.7 --- Detection --- p.30 / Chapter 2.9.7.1 --- Color detection --- p.30 / Chapter 2.9.7.2 --- Chemiluminescent detection --- p.31 / Chapter 2.9.8 --- Reprobing --- p.31 / Chapter 2.9.9 --- Colony hybridization --- p.31 / Chapter 2.10 --- Polymerase chain reaction (PCR) --- p.32 / Chapter 2.10.1 --- Arbitrarily primed polymerase chain reaction (AP- PCR) --- p.32 / Chapter 2.10.2 --- Random amplification of polymorphic DNA (RAPD) --- p.32 / Chapter 2.10.3 --- Amplification of ribosomal RNA gene (rDNA- PCR) --- p.33 / Chapter 2.11 --- Pulsed field gel electrophoresis --- p.33 / Chapter 2.11.1 --- Preparation of protoplasts --- p.33 / Chapter 2.11.2 --- Embedding of chromosomal DNAs --- p.34 / Chapter 2.11.3 --- Electrophoresis --- p.34 / Chapter 2.11.4 --- Southern blotting and hybridization --- p.35 / Chapter Chapter 3 --- Results --- p.36 / Chapter 3.1 --- Construction of a partial genomic library for Volvariella volvacea --- p.36 / Chapter 3.1.1 --- Genomic DNA purification and restriction enzyme digestion --- p.36 / Chapter 3.1.2 --- Preparation of vector arms --- p.36 / Chapter 3.1.3 --- Ligation and transformation --- p.36 / Chapter 3.2 --- Characterization of clones in the genomic library --- p.42 / Chapter 3.3 --- Fishing out ribosomal RNA gene from the genomic library by homologous rDNA probe --- p.45 / Chapter 3.4 --- Strain typing --- p.50 / Chapter 3.4.1 --- Strain typing by RFLPs using moderately repetitive probes --- p.50 / Chapter 3.4.2 --- Strain typing by PCR-based protocols: AP-PCR and RAPD --- p.50 / Chapter 3.4.3 --- Strain typing by PCR- RFLPs --- p.56 / Chapter 3.5 --- Electrophoretic karyotype analysis by pulsed field gel electrophoresis --- p.56 / Chapter 3.5.1 --- Protoplast preparation --- p.56 / Chapter 3.5.2 --- The electrophoresis condition --- p.56 / Chapter 3.5.3 --- Southern hybridization --- p.65 / Chapter Chapter 4 --- Discussion --- p.68 / Chapter 4.1 --- Genomic library --- p.68 / Chapter 4.2 --- Generation of molecular markers --- p.70 / Chapter 4.2.1 --- RFLPs --- p.70 / Chapter 4.4.2 --- AP-PCR and RAPD methods --- p.71 / Chapter 4.2.3 --- PCR- RFLP of rRNA gene --- p.72 / Chapter 4.2.4 --- Comparison of the four types of molecular markers --- p.72 / Chapter 4.3 --- Electrophoretic karyotype by PFGE --- p.74 / Conclusion --- p.80 / References --- p.81