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This diploma thesis with the name „Use of Functional Food in Cancer Therapy“ is focused on the study of the possibility of using nutraceuticals of functional food in cancer therapy. Literary part of this work deals with the topic of functional food and their positive effects on human body, especially in prevention and treatment of various type of diseases. One of the main topic is cancer and its treatment options. These days, research using nutraceuticals as a supplementary treatment or monotherapy modality of cancer is often the subject of research. In this work, the effect of nutraceutical curcumin, whose antitumor effects were tested in vitro in both monotherapy and supplementary breast cancer treatment, were investigated. As a supplementary treatment, curcumin was tested in synergy with the cytostatic doxorubicin. Biologically active molecules have been encapsulated into a cavity of protein nanocarrier apoferritin, which has been promoted to support molecule´s therapeutic effects while protecting non-transformed breast cells. The encapsulation efficiency was about 68–72 % for all applied molecules. Stability of encapsulated curcumin and doxorubicin complexes stored in aqueous medium at 4 °C and 20 °C for 48 hours was determined by monitoring the change in optical properties, the size and surface ζ-potential of the nanoparticles or premature drug release from the nanotransporter. In addition, a colloidal stability test have been confirmed the increased protection of biologically active molecules by the process of direct encapsulation. In solutions simulating the internal environment of the organism (blood plasma, cytoplasm, endosomes), the amount of the released molecules from the apoferritin cavity was monitored at 37 °C for 48 hours. The colloidal stability test determined the blood plasma behavior for 48 hours. The toxicity of the tested complexes against the tumor cell lines MDA-MB-231 and MDA-MB-468 was determined by several cell experiments. The HBL-100 cell line was consider as a control non-transformed cells. The research of this diploma thesis determined the oxidative stress of cell lines induced by prepared com-plexes and the rate of internalization of these formulations into cells. Biocompatibility was determined by monitoring protein corona formation on the surface of nanoparticles, activation of C3 complement component, genotoxic effects on healthy breast cell lines and hemolysis of erythrocytes in human blood.

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Title: The comparison of stereognosis and pallesthesia of the preferred and non-preferred upper extremity in students of physiotherapy Objectives: To the objectives of the work belong the processing and compilation of information concerning laterality and sensation in the region of the upper extremities, then to evaluate the preference of the upper extremity by means of elected tests in 30 students of physiotherapy and to evaluate, whether the sensitivity of stereognosis and pallesthesia is higher in the region of the non- preferred upper extremity than in the region of the preferred upper extremity or whether the sensitivity of stereognosis and pallesthesia both of the upper extremities do not differ. Methods: The tasks of the work were dealt with four tests directed to the examination of preference, stereognosis and pallesthesia in the region of upper extremities. The statistic analysis of the results of the examination was carried out by means of paired t-test. Results: In the examination of pallesthesia in the region of processus styloideus radii and in the examination of stereognosis the null-hypothesis, that the sensitivity of pallesthesia / stereognosis examined by selected test in the region of preferred and non-preferred upper extremity in students of physiotherapy does not differ, was at...

The diploma thesis aims on description and development of the pulmonary hospitals as a specific objects of landscape architecture. The subject has been divided into three basic areas. First area focuses on the specialised sanatoriums for tuberculosis treatment and their greenery as the main field of study from the landscape architecture point of view. As a part of this thesis we have created detailed graphical overview of the environmental and natural conditions as well as overview of the historical background for selected sanatoriums in the Czech republic. Second area aims to provide complex study of contemporary trends in health care facilities greenery identified in the specialised literature. As a part of this section we have compiled proposal for differentiation of the functional type "greenery of the health care facilities". Last area presents verification study for the expansion and cultivation of the OLU Paseka medical facility. The study is based on the upper-mentioned findings and emphasises on the application of contemporary medical care trends in the landscape engineering.

This diploma thesis is about soybean as functional food. Functional food contain components with positive effect on human health. In soybean there are lot of these components and very valued are phytoestrogens. Phytoestrogens has estrogenic activity with positive effect in prevention of osteoporosis, breast and prostate cancer, cardiovascular disease and menopausal problems. In experimental part was measured level of isoflavones in different kinds of soy food. The method for separation was high performance liquid chromatography with mass spectrometry.