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by Abraham Flexner and Frank P. Bachman. Introduced and annotated by Adeline and Murray Levine. / The two books included were originally published in 1916 and 1918 respectively. / Includes bibliographies.

No description available.

Lors de la transition G2/M des ovocytes de Xénope, la voie p39Mos-MEK1-MAPK présente des propriétés dynamiques et physiques particulières telles que l’ultrasensibilité, la bistabilité, l’irréversibilité et un caractère ‘tout-ou-rien’. Ces propriétés sont considérées dans le contexte de la boucle de rétroaction positive qui existe au sein de cette voie. L’objectif de cette thèse s’est focalisé sur le rôle de l’oncoprotéine p39Mos et le recrutement des motifs de régulation qui permettent l’apparition de ces propriétés. Des approches expérimentales et in silico ont été menées pour réaliser une modélisation physiquement et biologiquement réaliste de ce réseau. Le modèle développé tient compte de l’influence du MPF sur l’accumulation de p39Mos et ajuste le rôle de la boucle de rétrocontrôle positif. Par ailleurs, nous avons pu mettre en évidence que p90Rsk, cible des MAPK, est dégradée. La voie MAPK a été activée en absence de p39Mos. Nos résultats montrent que la 1,10 Phénanthroline monohydrate (1,10-PA) active les MAPK suivant une réponse graduelle et ultrasensible. L’action de la 1,10-PA s’exerce en absence de synthèse protéique et de toute boucle de rétrocontrôle, et nous avons émis l’hypothèse que la 1,10 PA agit via l’inactivation d’une MEK-phosphatase. Dans ce contexte, un modèle de pro-action est discuté et des inhibiteurs de phosphatases ont été utilisés pour activer les MAPK en absence de p39Mos. Nos résultats discutent du rôle de la boucle de rétroaction positive dans l’activation des MAPK et montrent que l’ultrasensibilité de réponse des MAPK peut être générée par des motifs de régulation de type pro-action. / During G2/M transition in Xenopus oocyte, p39Mos-MEK1-MAPK cascacade harbors specific dynamic and physical properties, such as ultrasensitivity, bistability, irreversibility, and all-or-none responses. These properties are generally considered in the context of the positive feedback loop that embeds the p39Mos-MEK1-MAPK pathway architecture. The objective of this work was focused onto p39Mos oncoprotein and regulation motifs recruitment enabling together the generation of such properties. Both experimental and in silico approaches were undertaken in order to yield a realistic modelisation, physically and biologically relevant for this network. We developed a model that takes into account the influence of MPF onto p39Mos accumulation, and adjusts the role of the positive feedback loop. Also, we were able to show that p90Rsk, target of MAPK, was degraded. This signaling pathway was activated in the absence of p39Mos. Our results show that 1,10 Phénanthroline monohydrate (1,10-PA) is able to induce gradual and ultrasensitive MAPK activation. 1,10-PA action is then exerted in the absence of protein synthesis and positive feedback loop. In this context, a feed forward loop model can be considered, and phosphatase inhibitors were used for MAPK activation in the absence of p39Mos. Our results confront the role attributed to the positive feedback loop in MAPK activation, and show that this ultrasensitive response may be generated in vivo through feed forward regulation motifs.

No description available.

We define the Lie group G2 and show several equivalent ways to view G2. We do the same with its Lie algebra g2. We identify a new basis for g2 using Bryant’s view of g2 and geometric considerations we develop. We then show how to construct a matrix representation of G2 given our particular basis for g2. We examine the geometry of 1 and 2-parameter subgroups of G2. Finally, we suggest an area of further research using the new geometric characterization we developed for g2.

M-theory, a generalization of string theory, motivates the search for examples of volume minimizing cycles in Riemannian manifolds of G2 holonomy. Methods of calibrated geometry lead to a system of four coupled nonlinear partial differential equations whose solutions correspond to associa- tive submanifolds of R7, which are 3-dimensional and minimize volume in their real homology classes. Several approaches to finding new solutions are investigated, the most interesting of which exploits the quaternionic structure of the PDE system. A number of examples of associative 3-planes are explicitly given; these may possibly be projected to nontrivial volume minimizing cycles in, for example, the G2-manifold R6 × S1.

Radio frequency identi cation (RFID) is a rapidly emerging technology that enables au- tomatic remote identi cation of objects. Passive and semi-passive RFID systems can be distinguished from other forms of wireless systems, because the RFID tags (transponders) communicate by way of backscatter. In addition, passive tags derive their energy from the RF energy emitted by the reader. RFID technology can provide a fully automated data capture and analysis system. Compared to a passive RFID system, an open platform semi-passive UHF RFID tag can provide identi cation, security, low-power (compared to a wireless sensor net- work(WSN)), medium range and medium processing speed. However, the eld of semi- passive RFID is still under development, and has yet there are no open development platforms available. This thesis develops a prototype of a semi-passive UHF RFID tag that is compatible with the leading UHF RFID standard EPCglobal Gen 2 Class 1. I alsot has the exible I2C and analog digital converter(ADC) interface, which allows the additional of external analog and digital sensors. The sensor data can be read by microcontroller and stored at memory. Standard reader can get sensor data by sending QUERY and READ command to tag. Test results of our open platform semi-passive UHF RFID tag demonstrated that it can achieve a read rate above 50% when an open platform semi-passive UHF RFID tag is placed four meters from the reader antenna and the reader output power is set to 21 dBm. In addition, the proposed semi-passive open platform RFID tag consumes very little power (4.9 mA in 2V with system frequency set to 8MHz).

Radio frequency identi cation (RFID) is a rapidly emerging technology that enables au- tomatic remote identi cation of objects. Passive and semi-passive RFID systems can be distinguished from other forms of wireless systems, because the RFID tags (transponders) communicate by way of backscatter. In addition, passive tags derive their energy from the RF energy emitted by the reader. RFID technology can provide a fully automated data capture and analysis system. Compared to a passive RFID system, an open platform semi-passive UHF RFID tag can provide identi cation, security, low-power (compared to a wireless sensor net- work(WSN)), medium range and medium processing speed. However, the eld of semi- passive RFID is still under development, and has yet there are no open development platforms available. This thesis develops a prototype of a semi-passive UHF RFID tag that is compatible with the leading UHF RFID standard EPCglobal Gen 2 Class 1. I alsot has the exible I2C and analog digital converter(ADC) interface, which allows the additional of external analog and digital sensors. The sensor data can be read by microcontroller and stored at memory. Standard reader can get sensor data by sending QUERY and READ command to tag. Test results of our open platform semi-passive UHF RFID tag demonstrated that it can achieve a read rate above 50% when an open platform semi-passive UHF RFID tag is placed four meters from the reader antenna and the reader output power is set to 21 dBm. In addition, the proposed semi-passive open platform RFID tag consumes very little power (4.9 mA in 2V with system frequency set to 8MHz).

Radio frequency identi cation (RFID) is a rapidly emerging technology that enables au- tomatic remote identi cation of objects. Passive and semi-passive RFID systems can be distinguished from other forms of wireless systems, because the RFID tags (transponders) communicate by way of backscatter. In addition, passive tags derive their energy from the RF energy emitted by the reader. RFID technology can provide a fully automated data capture and analysis system. Compared to a passive RFID system, an open platform semi-passive UHF RFID tag can provide identi cation, security, low-power (compared to a wireless sensor net- work(WSN)), medium range and medium processing speed. However, the eld of semi- passive RFID is still under development, and has yet there are no open development platforms available. This thesis develops a prototype of a semi-passive UHF RFID tag that is compatible with the leading UHF RFID standard EPCglobal Gen 2 Class 1. I alsot has the exible I2C and analog digital converter(ADC) interface, which allows the additional of external analog and digital sensors. The sensor data can be read by microcontroller and stored at memory. Standard reader can get sensor data by sending QUERY and READ command to tag. Test results of our open platform semi-passive UHF RFID tag demonstrated that it can achieve a read rate above 50% when an open platform semi-passive UHF RFID tag is placed four meters from the reader antenna and the reader output power is set to 21 dBm. In addition, the proposed semi-passive open platform RFID tag consumes very little power (4.9 mA in 2V with system frequency set to 8MHz).