Current practices support the use of probiotic and prebiotic supplementation to improve chronic gastrointestinal distress (GID). The aim of this study was to determine the tolerance and benefits of GlutenShield (GS), a prebiotic, probiotic, and enzyme supplement, on adults with GID. Subjects (n=20) took either GS or the placebo for 30 days and completed a pre-treatment FFQ as well as a pre- and post-treatment GID questionnaire, blood draw, and stool sample. Participants consumed more total and saturated fat, and less fiber and whole grains compared to the recommended intake. A significant reduction in IgG2 was observed in the GS group (p=0.008) as well as a significant reduction in self-reported bloating (p=0.038) with no change observed to cytokines or SCFAs (p>0.05). GS was well tolerated and perceived to be beneficial; however, further research is needed to identify the specific population of GID patients who could most benefit from GS supplementation.