This study evaluated the impact of acculturative stress and negative autostereotypes on the level of self-esteem and achievement motivation among subgroups of Hispanic college students. Subjects were classified by generational level as Second-generation (i.e., foreign-born parents), or Other (i.e., first-generation, foreign-born individuals, and third-generation, foreign-born grandparents;). By country/region of origin, subjects were divided into Central-Americans, Puerto-Ricans, Mexican, Mexican-Americans, and South Americans. Results showed that acculturative stress may facilitate loss of self-esteem particularly in Second-generation individuals, while negative autostereotypic attitudes may actually increase the student's level of motivation for achievement, particularly in Mexican-American individuals. Also, country/region of origin overall influenced negative autostereotypic attitudes.