Thèse de doctorat--Sciences naturelles--Faculté des sciences de Paris, 1908. N°: 1305. / Notes bibliogr.

Elicitation is one of possible methods for increasing the secondary metabolites production in plant cell cultures. This paper explores the potential effect of methylviologene on isoflavonoids production in Genista tinctoria L. suspension and callus cultures. Schenk and Hildebrandt nutrient medium was used with the addition of growth regulators 2,4- D in concentration of 5 mg/L and kinetin of 1 mg /L of medium. Elicitor was added in the form of ethanol solution at concentration of 2.19·10-2 mol l-1 , 2.19·10-3 mol l-1 , 2.19·10-4 mol l-1 . Samples were taken after 6, 12, 24, 48, 72 and 168 hours of elicitor exposure. Control samples were collected at 6, 24, 72 and 168 hours. The isoflavonoid content in suspension and callus cultures and in nutrient medium was evaluated by high performance liquid chromatography. The biggest increase of isoflavonoid content in callus culture was reached in daidzein content after 48 hours elicitor treatment in concentration of 2.19·10-3 mol l-1 (8.5 mg/g DW) and 2.19·10-4 mol l-1 (1.6 mg/g DW). The production of genistin, genistein and formononetin was slightly increased or zero compared to controls. Biochanin A was almost absent in the samples. The highest level of isoflavonoids was measured in the suspension culture after 48 hours elicitor application in the...

This study has the objective of identifying the possible environmental constraints that has role for the continuous loss of heathland plant Genista pilosa. The study has assessed different environmental settings where the plant occurs by way of overlaying analysis based on multiple spatial data sets. Thereafter empirical change detection analyses on the land use of the study area have been performed on the GIS environment by combining temporal based remotely sensed spatial data. The result was then analyzed using land use dynamicity model and the rates of change on each land use type are identified. Expansion of human activity, especially the spreading of agricultural land and urbanization, is found to be the most determinant factor for the dramatic loss of the plant. Finally serious attention for the protection of the plant is recommended by mentioning the possible problem that would occur due to a loss of biodiversity.

1 Abstract Elicitation method is one way to increase the production of secondary metabolites in in vitro of plant cultures and this work deals with this problem. Its aim was to find the positive effect of 2-hydroxy-4-(imidazo[1,2-a]pyrazine-2-carboxamide)benzoic acid from the group of pyrazine derivates on the production of isoflavonoids in in vitro cultures of Genista tinctoria L. Isoflavonoids play an important role in therapy of climacteric problems or they are promising substances of therapy various cancer types due to their affinity to estrogens β-receptors. This plant or rather its in vitro cultures are a potencial sources of isoflavonoids. Suspension and callus cultures of Genista tinctoria L. were used for elicitation. They were cultivated on Schenk & Hildebrant medium with the addition of 2,4-dichlorophenoxyacetic acid (2,4-D) as growth regulator at a concentration of 1 ml/l. The elicitor solution was added to the cultures at three concentrations: 3.3642 × 10-3 mol/l; 3.3642 × 10-4 mol/l; 3.3642 × 10-5 mol/l. The samples were taken at intervals of 6, 24, 48, 72 and 168 hours and then they were analyzed by HPLC method. At the same time intervals the samples of medium, where isoflavonoids were released, were analyzed. The biggest production of isoflavonoid was reached in suspension culture. The...

My thesis deals with dye plants and possibilities of their use. The first part contains classification of dye plants and history of their use. In the next part, there is a summary of dye plants and colors we can get from them. Methodology of coloring is also introduced. At the end, there are botanic parameters, environment needs and cultivation methods of eight selected dye plants. The thesis include database of dye plants in electronic form.