Two studies were conducted to compare nitrogen (N) and glutamate metabolism in Polypay and percentage White Dorper lambs.First, a two-phase digestion/N metabolism trial was conducted with 18 wether lambs of three genetic types: Polypay (PP), 1/2 White Dorper 1/2 Polypay (1/2 D), and 3/4 White Dorper 1/4 Polypay (3/4 D). Six lambs of each genetic type were fed a high roughage diet (HR; Phase 1) or high concentrate diet (HC; Phase 2). DM and N digestion was higher for 1/2 D than PP or 3/4 D fed HC. N retention was highest for 1/2 D regardless of diet.The second study analyzed the effect of genetic type on glutamate transporter and metabolizing enzyme expression in liver, kidney, longissimus dorsi muscle (LD muscle), and subcutaneous fat (Sub Q Fat) tissue of 18 wether lambs of three genetic types: PP, 1/2 D, and 15 /16 White Dorper 1/16 Polypay (15 /16 D). Tissue samples were analyzed for protein and mRNA content of GS, GDH, ALT, EAAC1, and GLT-1. Glutamate transport and metabolism capacity was lowest for the heavier muscled 15 /16 D lambs.The results suggest genetic type has an effect on N metabolism due to differential expression of glutamate transporters and metabolizing enzymes.

Compelling evidence suggests that major depression is associated with dysfunction of the brain glutamatergic transmission, and that the glutamatergic N-methyl-d-aspartate (NMDA) receptor plays a role in antidepressant activity. Recent post-mortem studies demonstrate that depression is associated with altered concentrations of proteins associated with NMDA receptor signalling in the brain. The present study investigated glutamate signalling proteins in the amygdala from depressed subjects, given strong evidence for amygdala pathology in depression. Lateral amygdala samples were obtained from 1314 pairs of age- sex-, and post-mortem-interval-matched depressed and psychiatrically healthy control subjects. Concentrations of NR1 and NR2A subunits of the NMDA receptor, as well as NMDA receptor-associated proteins such as post-synaptic density protein-95 (PSD-95) and neuronal nitric oxide synthase (nNOS) were measured by Western immunoblotting. Additionally, levels of enzymes involved in glutamate metabolism, including glutamine synthetase and glutamic acid decarboxylase (GAD-67), were measured in the same amygdala samples. NR2A protein levels were markedly and significantly elevated (+115%, p=0.03) in depressed subjects compared to controls. Interestingly, PSD-95 levels were also highly elevated (+128%, p=0.01) in the same depressed subjects relative to controls. Amounts of NR1, nNOS, glutamine synthetase, and GAD-67 were unchanged. Increased levels of NR2A and PSD-95 suggest that glutamate signalling at the NMDA receptor in the amygdala is disrupted in depression.